[Next-Generation Sequencing Analysis Case] Genotyping Analysis
By utilizing next-generation sequencing, it is possible to perform genotyping at a higher resolution! *Case study collection available.
Genome editing technology is a powerful tool that enables free genetic modification, but genotype analysis (genotyping) is an important step in selecting modified individuals. By evaluating allele frequencies in the F0 generation through next-generation sequencing, it is possible to select individuals for breeding. Additionally, in genome editing experiments with cultured cells, a diverse population of cells with various mutations can be obtained from a single experiment, and the nature of the mutations varies depending on the experimental conditions. Next-generation sequencing allows for detailed evaluation of the mutations in the cell population. [Case Overview] - Genotyping of cultured cells using CrispRvariant - After extracting DNA from cultured cell lines edited with CRISPR/Cas, target amplicon sequencing was performed using Miseq - The obtained data was analyzed using CrispRvariant *For more details, please refer to the PDF document or feel free to contact us.
- Company:ファスマック
- Price:Other