[Next-Generation Sequencing Analysis Case] COI Amplicon Analysis
We have found that it is an effective means to elucidate the relationship between hosts and parasites that is difficult to detect from appearance! *Case study collection is being distributed.
At the Kyoto University Center for Ecological Research, research is being conducted on the diversification of the genus Acer and the processes involved in coexistence among species. Our company has been commissioned to conduct part of the analysis, which we would like to introduce. In this analysis, we utilized host-specific blocking primers to suppress the amplification of host-derived genes, allowing for the efficient identification of the host, the leafroller moth, and a comprehensive exploration of parasites within it. We found that the analytical method using blocking primers is an effective means of elucidating the relationships between hosts and parasites that are difficult to detect visually. 【Case Overview】 ■ PCR was conducted in the presence and absence of blocking primers. ■ COI amplicon analysis was performed on 274 samples of leafroller moth larvae using Miseq. ■ From the obtained reads, we identified 10 species of leafroller moths and 13 species of parasitic wasps, clarifying the host-parasite relationships. *For more details, please refer to the PDF document or feel free to contact us.
- Company:ファスマック
- Price:Other