東ソー List of Products and Services

Tosoh Corporation has newly established a manufacturing plant for separation and purification agents at its Nanyo site, further enhancing its production capacity. To allow more people to use TOYOPEARL products, we will be conducting a half-price campaign. We hope you take this opportunity to discover the appeal of TOYOPEARL products and find them useful for your separation and purification needs. 【Campaign Overview】 ■ Duration: From Friday, August 1, 2025, to Friday, November 28, 2025 ■ Target Audience: All users ■ Eligible Products: TOYOPEARL products listed in the catalog, SkillPak (1mL, 5mL), and high-speed separation packing materials 　※ Please refrain from purchasing multiple units of the same product. *For more details, please download the PDF or feel free to contact us.

• Chromatographic resins and packing materials

"TOYOPEARL Super A" is a chromatography packing material for antibody purification that immobilizes genetically engineered Protein A. It has a high antibody adsorption capacity and alkali resistance, enabling the construction of an efficient and robust antibody purification process. Additionally, it allows elution under mild pH conditions, possesses excellent mechanical properties, and demonstrates good flow characteristics. 【Features】 ■ High antibody adsorption capacity (over 68g/L) ■ High alkali durability (compatible with 0.5mol/L NaOH cleaning) ■ Elution possible under mild pH conditions ■ Excellent mechanical properties and good flow characteristics *For more details, please refer to the PDF document or feel free to contact us.

• others

This catalog introduces various "Ion Chromatography" products. It features a high-speed ion chromatograph, the "IC-8100," which achieves high-throughput analysis, as well as a user-friendly workstation, the "IC-8100-WS," among many others. Additionally, it includes measurement data and eluent preparation manuals, making it a useful reference when considering implementation. [Contents (partial)] ■ High-speed ion chromatograph IC-8100 ■ Workstation IC-8100-WS ■ High-separation, high-performance columns for ion chromatography ■ Measurement data ■ Eluent preparation manual *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

The "TSKgel UP-SW series" is a size exclusion chromatography that is compatible with both UHPLC and HPLC systems. It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. The surface characteristics of the packing material are the same as the existing "TSKgel SW type." Additionally, we also offer the "TSKgel SuperSW mAb series," "UltraSW Aggregate," and "TSKgel SuperSW, SWXL, SW, BioAssistSWXL series." 【Features】 - Due to the use of fine particle packing materials (2μm/3μm), rapid analysis (15cm) and high-resolution analysis (30cm) of biomacromolecules are possible. - The surface characteristics of the packing material are the same as the existing TSKgel SW type. - Compatible with both UHPLC and HPLC systems. - It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

The "TSKgel SuperMultiporePW series" is a size exclusion chromatography that uses a porous, multimodal packing material. Three grades with different molecular weight fraction ranges are available, allowing for measurements from high molecular weight samples to low molecular weight and oligomers. The use of a highly hydrophilic packing material suppresses hydrophobic adsorption of samples. Additionally, we also offer the "TSKgel PWXL, PW series," "TSKgel PWXL-CP series," and "TSKgel SuperAW series" in our lineup. 【Features】 ■ Uses a porous, multimodal packing material ■ Excellent linearity of calibration curves enables highly reproducible molecular weight measurements ■ No inflection points in the calibration curve, preventing any irregularities in the chromatogram ■ The use of a highly hydrophilic packing material suppresses hydrophobic adsorption of samples *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

"TSKgel HIC-ADC Butyl" is a hydrophobic chromatography column (HIC) that introduces butyl groups to a non-porous filler (5μm). It has high separation selectivity for antibody-drug conjugates (ADCs) and is effective for drug-antibody ratio (DAR) analysis. It enables high-resolution analysis of antibody drugs and biopharmaceuticals (10cm column) as well as rapid analysis (3.5cm column). Additionally, it offers high protein recovery rates and allows for trace analysis. Please feel free to contact us for inquiries. 【Features】 ■ High-resolution analysis of antibody drugs and biopharmaceuticals (10cm column) and rapid analysis (3.5cm column) is possible. ■ High separation selectivity for antibody-drug conjugates (ADCs) and effective for drug-antibody ratio (DAR) analysis. ■ Stable quality (low lot-to-lot variation, high durability). ■ High protein recovery rates and capability for trace analysis. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

An overview of size exclusion chromatography (aqueous GPC) using aqueous solutions as the eluent is presented. We introduce the column "TSKgel PW series," which is commonly used in these measurements. 【Contents (excerpt)】 - SEC measurement of water-soluble polymers ■ Column selection ■ Eluent selection - SEC measurement of water-soluble synthetic polymers - SEC measurement of polysaccharides and oligosaccharides *For more details, please refer to the PDF document or feel free to contact us.

• Analytical Equipment and Devices

This is a technical document explaining the features of the new TOSOH SEC column "TSKgel UP-SW3000-LS," which addresses the phenomenon of non-specific adsorption of proteins (initial adsorption) often observed when equilibrating a new size exclusion chromatography (SEC) column with elution buffer and injecting protein samples. This phenomenon is particularly prevalent with antibody samples, where aggregates or dimers may adsorb, leading to a reduction in elution peaks or, in some cases, no peaks being eluted at all. Such occurrences are seen in nearly all SEC columns.

• Analytical Equipment and Devices

This document is a collection of test and analysis data for herbal medicines using TSKgel. In this data collection, examples of analysis for 20 items listed in the Japanese Pharmacopoeia, 18th edition, under the section for herbal medicines are introduced. 【Items included in the analysis】 1. … Onseiin extract 2. … Ougon 3. … Kōrenkydō extract 4. … Kakkontō extract 5. … Kamikihitō extract 6. … Kanzo 7. … Keishibukuryōgan extract 8. … Kōboku 9. … Gojūyutō extract 10. … Gorei-san extract 11. … Saiko 12. … Saikokaryūto extract 13. … Sanshishi 14. … Shakuyaku 15. … Ninjin 16. … Hanjakuhōbaku-tō extract 17. … Byakko kanjin-tō extract 18. … Botanpi 19. … Rokukonjitsutō extract 20. … Reikeijutsukan-tō extract

• Analytical Equipment and Devices

We will introduce examples of oligonucleotide separation using hydrophilic interaction chromatography (HILIC) and ion-pair reversed-phase chromatography.

• Analytical Equipment and Devices

The identification of critical quality attributes (CQAs) of antibody drugs, the establishment of their management scope, and their analysis can be effectively achieved using HPLC/UHPLC. We will introduce the TSKgel series (columns) used in the analysis of critical quality attributes and risk assessment of antibody drugs with UHPLC/HPLC.

• Analytical Equipment and Devices

This document is a technical material that explains the high-performance SEC column TSKgel UP-SW3000. We will introduce the basic characteristics and separation examples. [Contents (excerpt)] ■ Introduction ■ Basic characteristics of TSKgel UP-SW3000 ■ Application examples ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

• others

The "TSKgel(R) UP-SW3000-LS" is a size exclusion chromatography column for the separation of biomacromolecules, using a filler with a particle size of 2μm that has diol groups chemically bonded to the surface of silica gel. Due to the use of fine particle fillers (2μm), it enables rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins (antibodies, enzymes). It has minimal column shedding, making it suitable for measurements using light scattering detectors. 【Features】 ■ The surface characteristics of the filler are similar to the existing TSKgel SW type. ■ Minimal column shedding makes it ideal for measurements using light scattering detectors. ■ Improved filler and column materials reduce initial protein adsorption. ■ Shipping and storage solvents are easily managed with a 20% ethanol aqueous solution. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This document is a collection of data on size exclusion chromatography (SEC, GPC, GFC). (The SEC of biomacromolecules such as proteins is explained in detail in the "Bioseparation Handbook.") 【Contents】 Types and characteristics of columns Solvents used in SEC and their properties *Data examples* Polyolefins, PPS Synthetic rubber Styrene-based resins Polyvinyl chloride and vinyl chloride copolymers Polycarbonate Polyvinyl acetate, polyvinyl alcohol Methacrylic resins Polyamides Polyethers Polyesters Phenolic resins Urea resins, melamine resins, cresol resins Epoxy resins Polyurethanes Fluorine-based resins Cellulose derivatives and cellulose Organosiloxane-based polymers Cyanoacrylate resins Polysulfone, polyethersulfone Polyimide Polylactic acid, polycaprolactone Water-soluble polymers and polysaccharides *For more details, please refer to the PDF document or feel free to contact us.*

• Analytical Equipment and Devices

In general, plasmid DNA is obtained through purification from E. coli culture broth. The culture broth contains not only contaminating components such as host-derived genomic DNA, RNA, and proteins, but also open circular DNA (Open circular) and linear DNA, which are structural isomers of the target plasmid. Since these can reduce the efficiency of gene expression, clinical plasmid DNA is required to have a purity of over 90% for supercoiled DNA. Therefore, we examined the separation of structural isomers of plasmid DNA pBR322 using anion exchange chromatography, and we would like to introduce this study. *For more details, please refer to the PDF document or feel free to contact us.*

• Analytical Equipment and Devices

Perfluorinated compounds (PFCs) have been widely used in applications such as surfactants, surface treatment agents, and water repellents. However, due to their persistence and toxicity to living organisms, as well as their difficulty in degradation in the environment, they are regulated under the Stockholm Convention (POPs Treaty). Research is being conducted on alternative substances to long-chain alkyl PFCs, represented by PFOS and PFOA. Investigations are being carried out on the physical properties and actual conditions of PFCs with lower biological persistence, including short-chain alkyls, alkyl ethers, and cyclic structures. In this study, we introduce the results of establishing an analytical method using LC/MS and investigating a pretreatment method for environmental water samples, using a PFOS alternative substance with distinctive structural features as a model substance. *For more details, please refer to the PDF document or feel free to contact us.*

• Water quality testing

Perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), which are types of perfluorinated compounds (PFCs), are regulated under the "Stockholm Convention on Persistent Organic Pollutants (POPs Convention)." In Japan, as per the notification from the Director of the Water Supply Division of the Ministry of Health, Labour and Welfare (Notification No. 0330-1 to 4), the positioning of PFOS and PFOA in drinking water quality regulations was elevated from a matter requiring consideration to a water quality management target item starting in April 2020. The target value for these substances in water quality is set at a total of 50 ng/L. The testing method for water quality management target items (Target 31) specifies a method that combines solid-phase column pre-treatment with 1000-fold concentration and LC/MS measurement using reverse-phase chromatography. This report introduces an example of concentration pre-treatment and separation conducted in accordance with this testing method.

• Water quality testing

Using the ion chromatograph "IC-8100 series" and the high-performance IC column "TSKgel SuperIC-AnionHS," halide ions can be measured in as fast as 7 minutes. It may also be possible to accelerate combustion ion chromatography. For more details, please refer to the PDF document.

• Water quality testing

Introduction of a simultaneous analysis application for bromate and chlorate in sodium hypochlorite using the Tosoh Ion Chromatograph IC-8100 series - Bromate ions are analyzed using a post-column reaction UV detection method, while chlorate ions are analyzed with a conductivity detector in a single injection method - Achieved high separation of hypochlorite ions and bromate ions - The conductivity detector can also detect other anions - The post-column system is equipped with an automatic cleaning function, eliminating the need for routine maintenance

• Other physicochemical equipment

This document introduces an example of the analysis of bromate ions in sodium hypochlorite using the IC-8100 series and TSKgel SuperIC-WA column. The TSKgel SuperIC-WA column achieves high separation of chlorite ions and bromate ions, and it is possible to establish analytical conditions that allow for the detection of trace amounts of bromate ions by using a high-sensitivity post-column reaction system. *For more details, please refer to the PDF document or feel free to contact us.

• Water quality testing

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● Ideal for the separation of high molecular weight aggregates of antibodies, high molecular weight proteins, and nucleic acid fragments. ■ Quick analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

• others

This document introduces the analysis of drinking water quality standard items using the IC-8100 series and TSKgel SuperIC-WA columns. It presents conditions under which anions can be analyzed simultaneously with an electrical conductivity detector and bromate ions with a post-column reaction-UV absorbance detector. *For more details, please refer to the PDF document or feel free to contact us.*

• Water quality testing

This document is a technical material explaining the TSKgel SuperIC-WA column for high-performance ion chromatography. It introduces the basic properties of the packing material and column, separation characteristics, and application examples. [Contents (excerpt)] ■ Introduction ■ Product specifications list ■ Basic properties ■ Application examples - Analysis of tap water - Analysis of bromate and chlorate in sodium hypochlorite ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

• others

Optimized microparticle fillers (2µm, 3µm) enable rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes. The surface characteristics of the fillers are similar to those of the widely used general-purpose SWXL series columns. They are compatible with both UHPLC and HPLC systems.

• Analytical Equipment and Devices

This document is a technical material explaining the high-performance SEC column TSKgel UP-SW Aggregate. We will introduce its basic characteristics and separation examples. 【Contents (excerpt)】 ■ Introduction ■ Basic characteristics of TSKgel UP-SW Aggregate ■ Analysis examples - Analysis of heat-treated antibody aggregates - Analysis of IgM - Analysis of nucleic acids ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

• others

This document is a technical material that explains the high-performance SEC column TSKgel UP-SW2000. We will introduce its basic characteristics and separation examples. 【Contents (excerpt)】 ■ Introduction ■ Basic characteristics of TSKgel UP-SW2000 ■ Application examples ・ Separation of biopharmaceuticals ・ Separation of oligonucleotides ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

• others

The "IC-8100 Series" is an ion chromatograph that employs a unique high-speed, high-resolution column, allowing for the measurement of anions and cations in as little as 5 minutes per sample. With the automatic exchange-type gel suppressor system, high-sensitivity analysis can be performed continuously without being affected by contaminants in the sample. By connecting to a post-column reaction system, it is possible to measure drinking water standard items containing bromate in under 10 minutes. Analyses that were previously conducted under multiple conditions can now be performed in a single measurement. The auto-sampler comes standard with an automatic dilution function. Concentration measurements using a concentration column are also available as an option. Various optional units, such as automatic sample pretreatment units and automatic eluent supply units, allow for diverse system configurations. 【Features】 ■ Dedicated program included as standard for a comfortable measurement environment ■ Supports continuous analysis of 100 to 200 samples (IC-8100EX) ■ Auto-sampler with automatic dilution function (IC-8100EX) ■ Options can be combined to create a system tailored to your needs *You can view the product catalog from the "PDF Download" section. Please feel free to contact us for inquiries.

• Other measurement, recording and measuring instruments

SkillPak is a column filled with various TOYOPEARL, TSKgel PW series, and Ca++Pure-HA. The 1 mL and 5 mL types, which can be connected to general-purpose liquid chromatography systems with 1/16" size piping, are suitable for screening filling materials and method development. The 50 mL and 200 mL types feature flange connections and can be used for purification at a few grams scale and considerations for scale-up.

• others

This document is a technical material that explains ion chromatography (IC). We will introduce the basic principles of IC, measurement methods, sample preparation procedures, and actual measurement examples. 【Contents (excerpt)】 ■Separation Principles ・What is Ion Chromatography ■Measurement Methods ・Measurement Procedures ■Appendix-1 Application Data ■Appendix-2 Troubleshooting *For more details, please refer to the PDF document or feel free to contact us.

• others

Using the ion chromatograph "IC-8100 series" and the high-performance IC column "TSKgel SuperIC-AnionHS," carbonate ions elute at a position that does not overlap with the peaks of the standard seven anions. Therefore, even in samples where carbonate ions coexist at high concentrations, the quantification of other anions is not an issue. For more details, please refer to the PDF document "Technical Information: Ion Chromatography and the Effects of Carbonate Ions."

• Water quality testing

Using the ion chromatograph "IC-8100 series" and the high-performance IC column "TSKgel SuperIC-AnionHS," it is possible to measure 4 ppb of nitrite nitrogen in a sample containing 50 ppm of chloride ions with a system that uses only a conductivity detector. When using a UV absorbance detector, it is possible to measure trace amounts of nitrate nitrogen in samples containing higher concentrations of chloride ions. For more details, please refer to the PDF document 【Technical Data】Impact of High Concentration Chloride Ions on Nitrite Nitrogen.

• Water quality testing

In the analysis of fluoride ions using ion chromatography, depending on the analytical conditions, products generated by the addition of ethylenediamine to the sample may interfere with the peak of the fluoride ions. Similarly, the separation of silicate ions and fluoride ions is also important for the quantification of fluoride ions. Using the ion chromatograph "IC-8100 series" and the high-performance IC column "TSKgel SuperIC-AnionHS," it is possible to separate these interfering substances. For more details, please refer to the PDF document 【Technical Data】Ion Chromatography - Quantification of F Ions.

• Water quality testing

Introduction to the separation and quantification of nitrite nitrogen using ion chromatography with high-throughput columns (analysis within 10 minutes): - Measurement of nitrite nitrogen in drinking water - Measurement of nitrite nitrogen in the presence of excess chloride ions

• Other physicochemical equipment

For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

Both anions and cations are achieving short-time, high-resolution analysis using high-throughput columns.

• Other physicochemical equipment

The combustion decomposition-ion chromatography method involves absorbing gases generated by the combustion decomposition of a sample into an absorbent solution and measuring them using ion chromatography. This introduces an example of analysis using our high-throughput column in combustion decomposition-ion chromatography.

• Other physicochemical equipment

This document is a collection of application data from water quality tests using TSKgel. It introduces analytical examples of the main items for which the HPLC method is adopted in the notification (Ministry of Health, Labour and Welfare Notification No. 87, March 28, 2017) and the notice (Notification No. 0328-1, March 28, 2017) that specifies the analytical methods for water quality standard items and water quality management target setting items. [Contents (excerpt)] ■ Water quality standard items - Cyanide ions and cyanogen chloride - Nitrite nitrogen ■ Water quality management target setting items - Asulam, iprodione, cybutryn, thiofanox - Carbaryl (NAC) *For more details, please refer to the PDF document or feel free to contact us.

• Water quality testing

This data collection introduces analysis examples of the main items for which high-performance liquid chromatography and ion chromatography are used, among the testing methods listed in "Fertilizer Testing Methods (2019)." [Main harmful components and nitrate-reducing agents included in the analysis (excerpt)] ● Sulfamidic acid and ammonium thiocyanate (simultaneous analysis) ● Biuret nitrogen ● Dicyandiamide nitrogen ● 2-Amino-4-chloro-6-methylpyrimidine ● Melamine *For more details, please refer to the PDF document or feel free to contact us.

• Other environmental analysis equipment

■ High-speed analysis column "TSKgel FcR-IIIA-NPR" - Capable of recognizing differences in the N-linked glycan structures of the antibody Fc region and separating antibodies with different ADCC activities. - Liganded with recombinant human Fcγ receptor IIIa, offering improved stability compared to the native type. - Allows for direct separation of antibody samples without the need for purification or pre-treatment. - Separation can be achieved in a short time (20 minutes). (Including equilibration time, measurements can be made in 30 minutes per cycle.) ■ Preparative column "TSKgel FcR-IIIA-5PW" - Demonstrates equivalent separation selectivity as it uses the same ligand as TSKgel FcR-IIIA-NPR. - Capable of loading approximately 5 mg of antibody sample. - Allows for the collection of the amount necessary for structural analysis and activity measurement in a single preparative chromatography run. *For more details, please refer to the PDF document or feel free to contact us.*

• others

This document introduces the high-performance AFC column "TSKgel FcR-IIIA-NPR" handled by Tosoh Corporation. It includes the basic properties of the product, standard separation conditions, application examples, and usage precautions. The new measurement method using this column allows for very simple and quick results with good reproducibility. Additionally, this column can be used not only for quality control of antibody drugs but also for screening production cell lines, optimizing cell culture medium composition, and process analysis of culture processes. 【Contents (partial)】 ■ Introduction ■ Basic Properties - Properties of the packing material - Standard separation conditions - Effects of eluent composition - Effects of sample load amount - Durability - Cleaning methods - Lot-to-lot variation of packing material - Storage stability, etc. *For more details, please refer to the PDF document or feel free to contact us.

• others

"TSKgel Protein A-5PW" is an affinity chromatography column filled with a recombinant Protein A introduced into a porous hydrophilic polymer substrate. The substrate with a particle size of 20μm has high mechanical strength, allowing for high-speed analysis of IgG. It has a high IgG adsorption capacity and a wide quantification range. Additionally, the column material uses PEEK, resulting in low nonspecific adsorption. 【Features】 ■ The substrate with a particle size of 20μm has high mechanical strength, enabling high-speed analysis of IgG. ■ It has a high IgG adsorption capacity and a wide quantification range. ■ Durable column. ■ It has the same ligand as the separation and purification carrier TOYOPEARL AF-rProtein A HC-650F, providing similar separation selectivity. *For more details, please refer to the PDF document or feel free to contact us.

• others

This document is the product catalog for TOYOPEARL and TSKgel series. In "Process Chromatography for Antibodies," we present the characteristics of various chromatography separation modes, as well as key TOYOPEARL products for antibody purification, using tables. Furthermore, the product list categorizes numerous products under each item. [Contents (Excerpt)] <TOYOPEARL> ■ Process Chromatography for Antibodies Characteristics of various chromatography separation modes Key TOYOPEARL products for antibody purification Protein A and Protein L carriers Ion exchange carriers ■ Product List (Process Chromatography) *For more details, please refer to the PDF document or feel free to contact us.

• others

This document is a technical material that explains liquid chromatography. Liquid chromatography has become an essential method for the separation and analysis of biological samples. This guide provides a comprehensive explanation of various separation modes of liquid chromatography, their principles, and features, ranging from the basics to actual measurements. [Contents (excerpt)] - Properties of proteins and nucleic acids - Separation methods for biopolymers - Liquid chromatographs used for measuring biopolymers - Size exclusion chromatography - Ion exchange chromatography *For more details, please refer to the PDF document or feel free to contact us.

• others

The latest column is the UP-SW3000-LS. Please refer to that as well. The "TSKgel UP-SW3000" is a size exclusion chromatography column for the separation of biomacromolecules, using a packing material that chemically bonds diol groups to the surface of silica gel particles with a diameter of 2μm. Due to the fine particle packing material, rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes are possible. 【Features】 ■ Enables rapid analysis (15cm column) and high-resolution analysis (30cm) ■ Surface characteristics of the packing material are similar to existing TSKgel SW types ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments ■ Compatible with both UHPLC and HPLC systems *For more details, please refer to the PDF document or feel free to contact us.

• others

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● It is suitable for the separation of low molecular weight proteins, fragments, peptides, and oligonucleotides. 【Features】 ■ Rapid analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.*

• others