HIGUCHI INC. List of Products and Services

- As it is a granular product, it can suppress the scattering of fine powder. - It has excellent solubility. - It has performance equivalent to SCD medium. - Since it is individually packaged, there is no need for the hassle of measuring raw materials.

The powder flexible joint ConnflexⓇ developed by the Swiss powder equipment manufacturer Zimmermann Verfahrenstechnik is available. It is made from materials that excel in chemical resistance, heat resistance, and pressure resistance, and we can design products tailored to customer specifications and existing equipment, including size and connection parts (JIS standards). The flexible component materials include PTFE, polyurethane, and FEP. The standard material for the connecting parts is SUS316L, with Hastelloy available as an option.

H Media is a medium formulation for bacterial contamination testing of the aseptic (sterile filling) line that our company manufactures and sells domestically. It is granular with minimal dust generation and can be stored long-term (up to 2 years after production). There is no need for mixing or storing the individual medium raw materials. 【Features】 ■ Granular form to suppress fine powder dispersion ■ Excellent solubility ■ Performance equivalent to SCD medium ■ Individually packaged, eliminating the need for cumbersome weighing of raw materials *For more details, please refer to the materials available for download or contact us directly.

OligoHyaferre is a hydrolyzed sodium hyaluronate manufactured by a company in the Czech Republic, known for its excellent "anti-wrinkle effect," "moisture retention effect," and "skin barrier function recovery effect." In vitro studies have confirmed its effects of MMP inhibition, TNF-α inhibition, promotion of angiogenesis, and gene expression of HAS2. These effects lead to favorable results in in vivo "anti-wrinkle tests" and "skin barrier function recovery tests."

HyWhite is a sodium hyaluronate with whitening (brightening) properties manufactured by Contipro in the Czech Republic. HyWhite is a product that has high whitening (brightening) effects, made by partially replacing low molecular weight sodium hyaluronate with alpha-linolenic acid, which has high permeability to the skin. Alpha-linolenic acid is widely known for its strong melanin production suppression effects, and in in-vitro results, it has been confirmed to have a higher suppression effect than kojic acid. On the other hand, it is also known as a component that is difficult to formulate due to its low skin permeability and instability. This product promotes the absorption of alpha-linolenic acid, which has been a problem in the past, by replacing part of the low molecular weight hyaluronate with alpha-linolenic acid, thereby improving stability and maximizing the effects of alpha-linolenic acid. *In addition to HyWhite, Contipro also manufactures sodium hyaluronate, hydrolyzed sodium hyaluronate, and other hyaluronate derivatives.

It is possible to apply polymer coating to fine particles using newly developed dry coating technology. Compared to conventional water-based coatings, processing time can be significantly reduced.

Cross Linked HA is a hyaluronic acid cross-polymer made using Contipro's patented manufacturing method. The patented manufacturing technology creates a soft and fine gel, providing a unique sensation that is not found in conventional high molecular weight sodium hyaluronate. <About the Sensation> Immediately after application, it retains the pleasant texture of the chewy sodium hyaluronate, while the fine gel quickly penetrates the skin's grooves (such as wrinkles and pores), resulting in a moist yet refreshing sensation. <About Effectiveness> - Moisturizing effect (62% improvement) - Anti-wrinkle effect (46% reduction in wrinkles) - Sustained release effect of active ingredients (51% increase in the activity of Vitamin B3)

Bridge-type rice starch RICE PO4 has an average particle diameter of 8μm and a sharp particle size distribution. It is insoluble in water and does not undergo thermal modification, making it an excellent particle for formulation. It has high oil absorption capacity and can be used to improve the feel of products such as sebum adsorbents and creams (reducing stickiness).

<Ectoin Natural Features> Ectoin is a type of cyclic amino acid produced by extremophilic microorganisms (Halomonas) that inhabit salt lakes and deserts. It has 4 moles of bound water for every 1 mole of ectoin, giving it cosmotropic properties. This characteristic contributes to the stabilization of interactions between water molecules and forms a hydrocomplex. This hydrocomplex helps stabilize biological structures (such as cells, proteins, enzymes, and nucleic acids) against environmental stresses like temperature changes, high salt concentrations, strong UV exposure, and dehydration. ― About Effectiveness ― In Vivo Vaginal care effects: Reduction of vaginal atrophy symptoms, vaginal moisturization (50% improvement in dryness), improvement of vaginal heat and itching. Moisturizing persistence (lasting for 1 week), barrier recovery, erythema suppression, protection of Langerhans cells (100% protection). Anti-aging effects: Improvement in skin roughness (86% improvement), wrinkle depth (32% improvement), elasticity (82% improvement), smoothness (61% improvement), whitening effect (25% improvement). In Vitro: Promotion of heat shock protein production, anti-pollution effects (Ex-Vivo), protection against blue light, UV-A, UV-B, and IR-A.

Glycoin natural is a product composed of 100% unique structural isomers manufactured using patented biotechnology by Bitop AG in Germany. These structural isomers are found only in the miraculous resurrection plant Myrothamnus flabellifolia and blue-green algae (spirulina), which are known as the miracle plants of the desert that can revive green with just a small amount of water even after being dried for 50 years. Glycoin natural activates the rejuvenating power of tired skin, stressed skin, and aging skin. <Efficacy> Stimulates cell regeneration, growth factors, and ATP synthesis Activates antioxidant enzymes (SOD1, SOD2, CAT) Immediate moisturizing effect improved by 49% (after one application) Skin elasticity improved by 93% Skin smoothness improved by 61% Skin thickness increased by 9.9% Reduces sunburn and skin redness Tissue repair and wound healing effects maintained for 48 hours

Himantalia elongata is a bamboo-like seaweed that grows on the seabed at depths of 10 to 20 meters in the Brittany region of France. Marine Bamboo grows to lengths of over 3 meters and is named for its shape. Marine BambooTM is a patented lipophilic active substance, a product derived from the extraction of fucosterol and phycocyanin from Himantalia elongata. Marine BambooTM is an excellent product for absorbing UV, blue light, and IRA. Its high absorption effect, particularly for blue light, is attributed to the extreme environment at its habitat depth. In Vitro: - Inhibition effect on MMP1 gene expression: -38% - Stimulation of elastin gene expression: +40% - Blue light protection effect: Damage reduction effect on fibroblasts: +23% Inhibition effect on MMP1 production: -26% In Vivo: - Tightening effect: +8% - Smoothing effect: +12% - Tone improvement effect: +4.1%

Pearl Alga is a fraction of fluorotannins and other components extracted from Sargassum muticum, a brown alga known as pearl algae. The coastal waters of Brittany, France, where pearl algae inhabit, have a high probability of organisms attaching to and parasitizing seaweeds due to the presence of many estuaries, making it a sea with a much higher likelihood of such occurrences compared to the open ocean. However, Odycea discovered that pearl algae are not affected by this phenomenon. The reason is that this alga produces unique substances that have convergent, antibacterial, and antifungal properties. These substances include high concentrations of gallic acid (an average of 3 mg/100 g) and fluorotannins. As a result, the surface of pearl algae remains clean and free from parasitic organisms. Due to the effects of these substances, Pearl Alga can purify the skin and provide excellent benefits for skin care, including pore care, suppression of sebum secretion, and reduction of shine. - Efficacy - - Reduction in pore density, diameter, and surface area - Improvement in skin smoothness - Suppression of sebum secretion - Reduction of shine

Schizophyllan is a water-soluble high molecular weight polysaccharide produced in the cell walls of the fungus Schizophyllum commune. It can regulate various types of cellular responses to external stimuli and influence cell survival rates and protein synthesis. Skin aging caused by UV stress can lead to a deterioration of immune processes, resulting in the formation of wrinkles and dehydration. Schizophyllan has been confirmed to improve skin moisture levels and reduce wrinkles by regulating the underlying immune processes. <Efficacy> =In Vitro= Improvement in cell survival rate: 40% increase Promotion of TGF-β production: 150% increase Enhancement of IL-6 production: 60% increase Enhancement of IL-1beta production: 20% increase Inhibition of MMP1 expression (UV irradiation): 40% inhibition =In Vivo= Barrier function recovery effect Increase in skin moisture levels Suppression of skin erythema Improvement of crow's feet wrinkles

Carboxymethyl glucan (CMG) is a derivative of β-glucan isolated from yeast cell walls and is a natural yeast polysaccharide. While β-glucan is insoluble, this product has been made water-soluble while maintaining its biological activity through carboxymethylation. CMG significantly increases superoxide dismutase (SOD) activity, which shows important immune-regulating and antioxidant effects. These mechanisms help protect the skin from UV stress. = In Vitro = - Maintenance of glutathione pool (UV) - Promotion of SOD (reactive oxygen species-decomposing enzyme) production (UV): 40% increase - Inhibition of cellular phospholipid oxidation (UV): 80% inhibition - Inhibition of cellular protein oxidation (UV, vs TiO2): 22% inhibition - Inhibition of inflammatory cytokine (IL-6) (UV): 20% inhibition - Inhibition of inflammatory cytokine (IL-1beta) (UV): 40% inhibition - Promotion of anti-inflammatory cytokine (IL-10) production (UV): 60% increase = In Vivo = - Increase in collagen fibers and dermal papilla cells - Improvement in skin smoothness - Reduction in pore size - Improvement in skin elasticity - Improvement in crow's feet wrinkles

TanActine (glucomannan) is a yeast cell wall polysaccharide extracted from the culture of the yeast Candida utilis. TanActine is a yeast cell wall polysaccharide that has been shown to reduce skin redness and maintain melanin production. Data also suggests that TanActine may help in the repair of skin cells and protect against UV-induced cellular and DNA damage. <Efficacy> =In Vitro= - Promotion of melanocyte-stimulating hormone stimulation: 40% increase - Suppression of cell death due to UV irradiation - Improvement of keratinocyte cell survival rate: 10% improvement - Suppression of apoptosis: 30% suppression - DNA protective effect through inhibition of thymine dimer formation (UV irradiation): 60% suppression - Suppression of inflammatory cytokine (IL-1α) (UV irradiation): 50% suppression - Suppression of inflammatory cytokine (PGE2) (UV irradiation): 50% suppression =In Vivo= - Suppression of erythema (UV irradiation)

Krodesin is a nonapeptide (synthetic human nonapeptide-4) that mimics the active site of the anti-aging gene Klotho. The Klotho gene decreases with age and has been proven to have anti-aging effects. The name is derived from Klotho, the goddess in Greek mythology who spins the thread of fate. Klotho can protect the skin from the fate of aging and provide true rejuvenating effects. =In Vitro= Inhibition of insulin signaling (100%) Stimulation of SIRT1 gene expression (45%) Reduction of reactive oxygen species induced by peroxides (-10%) Inhibition of cellular aging Suppression of UV-induced inflammatory cytokine IL6 (-35%) =In Vivo= Anti-wrinkle (-50%) Fig.1 Elasticity (+200%), Pore size (-10%), number (-8%), Dermal papillae (+15%), luster (+20%), Sebum suppression (-10%)

BOREALG TM is a fat-soluble active substance derived from a large brown kelp called Laminaria hyperborea, known as the queen of longevity. Laminaria hyperborea inhabits the rocky surfaces of coastal areas. It has a strong adaptability to withstand the impact of powerful waves crashing against the reefs. In Brittany, for instance, when winter winds reach 130 km/h, the coastline is exposed to waves exceeding 10 meters. The pressure of the waves exerts 12 tons per square meter on the rocks and kelp forests. Odycea discovered that Laminaria hyperborea possesses ultimate restorative abilities to counteract strong waves. Borealg TG collected data focusing on the leptin receptor gene, fatty acid binding protein 3 gene, and Cyclin D1 gene, which are related to skin regeneration, activation, and rejuvenation processes. - Increase in leptin receptor gene expression stimulation: 139% improvement - Increase in fatty acid binding protein 3 gene expression stimulation: 220% improvement - Suppression of Cyclin D1 gene expression stimulation: -36% - Mitochondrial survival rate: 18% improvement - Suppression of erythema: -10%

MARINOVA is a biotechnology company established in 2003 in Tasmania, Australia. It is the manufacturer of the world's only high-purity organic fucoidan extract derived from brown algae, using its unique green chemistry technology. The company's product, MARITECH BRIGHT, is made from sustainable raw materials. =Sustainable Manufacturing Process= 1) Sustainable Sourcing: Wild seaweed is sourced from four regions around the world, employing harvesting methods that consider regeneration. 2) Zero Waste: Renewable energy (zero CO2 emissions) is used, and waste is utilized in agriculture. 3) Extraction with water, with 50% being reused. =About the Product= - Ingredients: Contains ≥90% fucoidan and polyphenols - Efficacy: 1) Anti-aging functions - Improves skin radiance - Reduces wrinkle depth - Stimulates expression of anti-aging gene SIRT1 - Inhibits glycation (50%) - Suppresses collagenase, tyrosinase, and elastase by up to 99% 2) Whitening functions - Improves pigmentation (20%) 3) Antioxidant functions - Removes free radicals - Against UVA/B: Suppresses erythema and TEWL caused by irradiation - Oxygen radical absorption capacity (twice that of VC)

- Ingredients: Contains Fucoidan ≧ 85% - Efficacy: 1) Anti-aging function - Wrinkle depth: -9% - Stimulation of anti-aging gene SIRT1 expression (+28%) - Glycation inhibition effect (-50%) - Inhibition of collagenase and elastase by up to 99% 2) Protection effect - Suppression of erythema after UV exposure by 45% - Antiviral effect (multiple including herpes simplex) 3) Moisturizing and smoothing - Increase in moisture by 30% after UV exposure - Increase in stimulation of skin cell expression after UV exposure - Inhibition of myeloperoxidase by up to 99%

HyRetin is a product that introduces retinoids to low molecular weight sodium hyaluronate, enhancing the stability and skin permeability of retinoic acid. =In Vitro= - Anti-inflammatory effects: IL-8: -15%, IL-6: -40% - Promotion of collagen type 1 production: +40% - Promotion of fibronectin production: 1.4 times (vs control) =In Vivo= - Dermal density: +10% improvement - Number of dermal papilla cells: +48% - Improvement in skin elasticity: -34% (recovery time) - Reduction in wrinkle depth: -36% - Skin radiance: +43% - Skin moisture level: +7% - Erythema suppression effect: -6% - Sebum reduction effect: -36% - Pore size: -25% - Anti-acne effect: size: -50%, number: -68.75% and more.

The Baobab (scientific name: Adansonia) is a deciduous tree native to regions such as Africa and Madagascar, belonging to the Malvaceae family and the Baobab genus. In Africa, its fruit has been consumed as a panacea since ancient times and is referred to as the "Tree of Life." The trunk of the Baobab tree has a shape resembling a sake bottle, is hollow inside, and can store up to 10 tons of moisture in its trunk, allowing it to survive by shedding its leaves and entering dormancy during the dry season, relying on the moisture stored within its trunk. LIFTISSTM is an extract obtained from the fruit of this Tree of Life, and it is a gel-like product rich in organic acids (AHA) and natural moisturizing factors (NMF), containing natural sugar components (77% galacturonic acid). <Mechanism of Action> Organic acids (alpha-hydroxy acids) break down keratin on the skin's surface, promoting exfoliation and epidermal regeneration, resulting in smoother skin and providing moisturizing and wrinkle-smoothing effects. Natural sugars (components of NMF), such as glucose and xylose, bind with keratin to form complexes that attract water molecules, thereby providing moisturizing effects. Additionally, natural sugars form a film on the epidermis, creating a tightening effect. <Efficacy> - Number of wrinkles: -41% - Area of wrinkles: -24% - Lifting effect, etc.

This product is an extract derived from the new buds of linden using GREENTECH's patented cryo-extraction technology. Linden is a long-lived tree that can live for centuries and was considered a sacred tree in ancient Germany. The extract of its flowers has anti-inflammatory and protective properties and has been used for various purposes. In particular, the new buds consist of embryonic tissue from the proliferation process, are full of vitality, and are rich in active components necessary for growth, such as plant hormones, proteins, amino acids, and sugars. The company's patented technology allows for the preservation and extraction of the active components of the new buds without damage. <Mechanism> It forms a film on the surface of the hair, suppressing moisture release, protecting the cuticle, smoothing the hair surface, and making it less prone to tangling. Additionally, by simultaneously providing flavonoids, the helical bonds of keratin (the fibrous protein that makes up 95% of hair) are stabilized. Furthermore, using free sugars (components of natural moisturizing factors) forms a water complex in the upper layer of the scalp, providing constant hydration. <Efficacy> - Improvement in hair moisture levels: +83% - Improvement in scalp moisture levels: +33% - Cuticle improvement - Volume enhancement - Static electricity prevention

The structure of hair fibers is the most important factor for the quality and health of hair. When the fiber structure deteriorates, the shine and smoothness of the hair decrease. Hair is constantly exposed to external stress factors such as sunlight and pollutants. These stress factors are referred to as "exposome." The structure of hair fibers deteriorates due to the exposome, leading to a decrease in the mechanical strength, shine, and smoothness of the hair. ZORYALYS is a raw material composed of three extracts developed to protect hair from the exposome. The extracts used are derived from ginger root, magnolia bark, and placasia, each serving a specific role: ginger provides antioxidant functions, magnolia offers protection against light damage, and placasia oil contains components similar to sebum in hair, improving hair quality. In Vivo (Caucasian, Asian) - Cortex protection against UV-A, cuticle protection, overall hair protection (evaluation of carbonylation) - Protection of keratin fiber structure against xenon (sunlight), prevention of tryptophan (melanin precursor) decomposition, prevention of cysteine participation, maintenance of mechanical strength, improvement of shine (twice as much), prevention of hair color fading.

- Circadian Rhythm Circadian rhythm is a physiological phenomenon that regulates certain processes in the body on an approximately 25-hour cycle. Most processes in the body, including wakefulness rhythm, autonomic nervous system, endocrine hormone system, immune system, and metabolic system, are regulated by circadian rhythms. Disruptions in these rhythms are correlated with numerous diseases (obesity, cancer, new vascular diseases, diabetes, sleep disorders, etc.) and skin conditions (dark circles, wrinkles, fatigue, etc.). - Circadian Rhythm Monitor Cells This product is a monitor cell developed to find components that regulate circadian rhythms. It is a mouse embryonic fibroblast that can measure the expression rhythm of the bmal1 gene, one of the clock genes that form the body clock. It is derived from a transgenic mouse (Bmal1-ELuc mouse) created using a construct that links the luminescent protein Eluc downstream of the bmal1 gene promoter, prepared from mouse embryos. - Details Cell Name: Circadian Rhythm Evaluation Cells (Bmal1-Eluc MEF) Origin: Mouse Cell Count: 5.0×10^5 cells/vial Storage Method: Liquid nitrogen tank Ultra-low temperature freezer (-140°C) Deep freezer (-80°C)

Hawthorn Cosmelean is an extract from the flowers of the Western Hawthorn. It contains active ingredients such as vitexin-2-O-rhamnoside: 0.07-0.11%. <Mechanism of Action> - The flavonoids contained in Cosmelean Hawthorn reduce congestion in capillaries and alleviate erythema. - By promoting blood flow, it improves the oxygen levels in the skin. This activates a constant process. <Efficacy> Reduces redness in the skin and brightens it. Unifies skin tone and enhances transparency. Reduces skin discomfort/unpleasant sensations (tightness due to dryness, inflammation). In Vivo: Redness reduction (IR-induced): -6% Redness reduction (UV-induced): -7%

FabRx, a UK company specializing in pharmaceuticals and biotechnology that spun out from University College London, has launched a 3D printer aimed at the manufacturing of solid dosage forms focused on personalized medicine. The Printlet, a tablet manufactured using a 3D printer with three different nozzles (for powder, gel, and filament), has been tested in various research institutions, hospitals, and pharmacies overseas, and is now available for sale domestically. By using registered formulations and manufacturing conditions in dedicated software, it can be applied in medical settings for personalized medicine, as well as in drug discovery and clinical trial drug manufacturing.