HIGUCHI INC. List of Products and Services

Contipro is a biotechnology company located in the Czech Republic. It is a global manufacturer that produces and sells various active ingredients, primarily sodium hyaluronate, in the pharmaceutical and cosmetic fields, supplying to over 60 countries for approximately 30 years. The listed products are categorized into three groups: sodium hyaluronate, natural polysaccharides, and peptides. <Sodium Hyaluronate> We offer grades with a molecular weight distribution ranging from 2.3 million Da to 10,000 Da, and we have confirmed differences in efficacy due to varying skin permeability based on molecular weight through Vivo and Vitro data. Additionally, we also have hydrolyzed sodium hyaluronate as a grade with excellent skin permeability. <Derivatives> We have confirmed through Vivo and Vitro data that sodium hyaluronate possesses not only its inherent properties of moisturizing, barrier function, anti-wrinkle, and calming effects but also efficacy in whitening, sebum secretion regulation, sebum suppression, and improvement of skin texture. *For more details, please download the PDF or feel free to contact us.

HyWhite is a sodium hyaluronate with whitening (brightening) properties manufactured by Contipro in the Czech Republic. HyWhite is a product that has high whitening (brightening) effects, made by partially replacing low molecular weight sodium hyaluronate with alpha-linolenic acid, which has high permeability to the skin. Alpha-linolenic acid is widely known for its strong melanin production suppression effects, and in in-vitro results, it has been confirmed to have a higher suppression effect than kojic acid. On the other hand, it is also known as a component that is difficult to formulate due to its low skin permeability and instability. This product promotes the absorption of alpha-linolenic acid, which has been a problem in the past, by replacing part of the low molecular weight hyaluronate with alpha-linolenic acid, thereby improving stability and maximizing the effects of alpha-linolenic acid. *In addition to HyWhite, Contipro also manufactures sodium hyaluronate, hydrolyzed sodium hyaluronate, and other hyaluronate derivatives.

OligoHyaferre is a hydrolyzed sodium hyaluronate manufactured by a company in the Czech Republic, known for its excellent "anti-wrinkle effect," "moisture retention effect," and "skin barrier function recovery effect." In vitro studies have confirmed its effects of MMP inhibition, TNF-α inhibition, promotion of angiogenesis, and gene expression of HAS2. These effects lead to favorable results in in vivo "anti-wrinkle tests" and "skin barrier function recovery tests."

Cross Linked HA is a hyaluronic acid cross-polymer made using Contipro's patented manufacturing method. The patented manufacturing technology creates a soft and fine gel, providing a unique sensation that is not found in conventional high molecular weight sodium hyaluronate. <About the Sensation> Immediately after application, it retains the pleasant texture of the chewy sodium hyaluronate, while the fine gel quickly penetrates the skin's grooves (such as wrinkles and pores), resulting in a moist yet refreshing sensation. <About Effectiveness> - Moisturizing effect (62% improvement) - Anti-wrinkle effect (46% reduction in wrinkles) - Sustained release effect of active ingredients (51% increase in the activity of Vitamin B3)

Bridge-type rice starch RICE PO4 has an average particle diameter of 8μm and a sharp particle size distribution. It is insoluble in water and does not undergo thermal modification, making it an excellent particle for formulation. It has high oil absorption capacity and can be used to improve the feel of products such as sebum adsorbents and creams (reducing stickiness).

<Ectoin Natural Features> Ectoin is a type of cyclic amino acid produced by extremophilic microorganisms (Halomonas) that inhabit salt lakes and deserts. It has 4 moles of bound water for every 1 mole of ectoin, giving it cosmotropic properties. This characteristic contributes to the stabilization of interactions between water molecules and forms a hydrocomplex. This hydrocomplex helps stabilize biological structures (such as cells, proteins, enzymes, and nucleic acids) against environmental stresses like temperature changes, high salt concentrations, strong UV exposure, and dehydration. ― About Effectiveness ― In Vivo Vaginal care effects: Reduction of vaginal atrophy symptoms, vaginal moisturization (50% improvement in dryness), improvement of vaginal heat and itching. Moisturizing persistence (lasting for 1 week), barrier recovery, erythema suppression, protection of Langerhans cells (100% protection). Anti-aging effects: Improvement in skin roughness (86% improvement), wrinkle depth (32% improvement), elasticity (82% improvement), smoothness (61% improvement), whitening effect (25% improvement). In Vitro: Promotion of heat shock protein production, anti-pollution effects (Ex-Vivo), protection against blue light, UV-A, UV-B, and IR-A.

Glycoin natural is a product composed of 100% unique structural isomers manufactured using patented biotechnology by Bitop AG in Germany. These structural isomers are found only in the miraculous resurrection plant Myrothamnus flabellifolia and blue-green algae (spirulina), which are known as the miracle plants of the desert that can revive green with just a small amount of water even after being dried for 50 years. Glycoin natural activates the rejuvenating power of tired skin, stressed skin, and aging skin. <Efficacy> Stimulates cell regeneration, growth factors, and ATP synthesis Activates antioxidant enzymes (SOD1, SOD2, CAT) Immediate moisturizing effect improved by 49% (after one application) Skin elasticity improved by 93% Skin smoothness improved by 61% Skin thickness increased by 9.9% Reduces sunburn and skin redness Tissue repair and wound healing effects maintained for 48 hours

Odesia: A French cosmetic ingredient manufacturer that offers products centered around extracts from algae native to the Brittany region of France. The company's products apply the special defensive properties that allow algae to survive in various harsh natural environments to cosmetics. Jade Alga TM: A patented active ingredient rich in native ulvan (sulfated polysaccharide) derived from the green seaweed of the Enteromorpha genus found along the Brittany coast. It is the only algae with "shape memory capability." The Enteromorpha in Brittany is significantly affected by the tides that occur every six hours. Its shape memory capability allows it to withstand repeated cellular deformation (contraction and expansion) caused by the tides without damage. Sustainability efforts: The large Enteromorpha has proliferated due to pesticide runoff and global warming, becoming a social issue. Jade Alga is a cosmetic ingredient that utilizes this large Enteromorpha. <Efficacy> - Maintenance and recovery of normal stratum corneum arrangement (shape memory technology) - Soft peeling effect - Improved skin plasticity and flexibility - Photoprotection effect - Antioxidant effect

Himantalia elongata is a bamboo-like seaweed that grows on the seabed at depths of 10 to 20 meters in the Brittany region of France. Marine Bamboo grows to lengths of over 3 meters and is named for its shape. Marine BambooTM is a patented lipophilic active substance, a product derived from the extraction of fucosterol and phycocyanin from Himantalia elongata. Marine BambooTM is an excellent product for absorbing UV, blue light, and IRA. Its high absorption effect, particularly for blue light, is attributed to the extreme environment at its habitat depth. In Vitro: - Inhibition effect on MMP1 gene expression: -38% - Stimulation of elastin gene expression: +40% - Blue light protection effect: Damage reduction effect on fibroblasts: +23% Inhibition effect on MMP1 production: -26% In Vivo: - Tightening effect: +8% - Smoothing effect: +12% - Tone improvement effect: +4.1%

Pearl Alga is a fraction of fluorotannins and other components extracted from Sargassum muticum, a brown alga known as pearl algae. The coastal waters of Brittany, France, where pearl algae inhabit, have a high probability of organisms attaching to and parasitizing seaweeds due to the presence of many estuaries, making it a sea with a much higher likelihood of such occurrences compared to the open ocean. However, Odycea discovered that pearl algae are not affected by this phenomenon. The reason is that this alga produces unique substances that have convergent, antibacterial, and antifungal properties. These substances include high concentrations of gallic acid (an average of 3 mg/100 g) and fluorotannins. As a result, the surface of pearl algae remains clean and free from parasitic organisms. Due to the effects of these substances, Pearl Alga can purify the skin and provide excellent benefits for skin care, including pore care, suppression of sebum secretion, and reduction of shine. - Efficacy - - Reduction in pore density, diameter, and surface area - Improvement in skin smoothness - Suppression of sebum secretion - Reduction of shine

Schizophyllan is a water-soluble high molecular weight polysaccharide produced in the cell walls of the fungus Schizophyllum commune. It can regulate various types of cellular responses to external stimuli and influence cell survival rates and protein synthesis. Skin aging caused by UV stress can lead to a deterioration of immune processes, resulting in the formation of wrinkles and dehydration. Schizophyllan has been confirmed to improve skin moisture levels and reduce wrinkles by regulating the underlying immune processes. <Efficacy> =In Vitro= Improvement in cell survival rate: 40% increase Promotion of TGF-β production: 150% increase Enhancement of IL-6 production: 60% increase Enhancement of IL-1beta production: 20% increase Inhibition of MMP1 expression (UV irradiation): 40% inhibition =In Vivo= Barrier function recovery effect Increase in skin moisture levels Suppression of skin erythema Improvement of crow's feet wrinkles

Carboxymethyl glucan (CMG) is a derivative of β-glucan isolated from yeast cell walls and is a natural yeast polysaccharide. While β-glucan is insoluble, this product has been made water-soluble while maintaining its biological activity through carboxymethylation. CMG significantly increases superoxide dismutase (SOD) activity, which shows important immune-regulating and antioxidant effects. These mechanisms help protect the skin from UV stress. = In Vitro = - Maintenance of glutathione pool (UV) - Promotion of SOD (reactive oxygen species-decomposing enzyme) production (UV): 40% increase - Inhibition of cellular phospholipid oxidation (UV): 80% inhibition - Inhibition of cellular protein oxidation (UV, vs TiO2): 22% inhibition - Inhibition of inflammatory cytokine (IL-6) (UV): 20% inhibition - Inhibition of inflammatory cytokine (IL-1beta) (UV): 40% inhibition - Promotion of anti-inflammatory cytokine (IL-10) production (UV): 60% increase = In Vivo = - Increase in collagen fibers and dermal papilla cells - Improvement in skin smoothness - Reduction in pore size - Improvement in skin elasticity - Improvement in crow's feet wrinkles

TanActine (glucomannan) is a yeast cell wall polysaccharide extracted from the culture of the yeast Candida utilis. TanActine is a yeast cell wall polysaccharide that has been shown to reduce skin redness and maintain melanin production. Data also suggests that TanActine may help in the repair of skin cells and protect against UV-induced cellular and DNA damage. <Efficacy> =In Vitro= - Promotion of melanocyte-stimulating hormone stimulation: 40% increase - Suppression of cell death due to UV irradiation - Improvement of keratinocyte cell survival rate: 10% improvement - Suppression of apoptosis: 30% suppression - DNA protective effect through inhibition of thymine dimer formation (UV irradiation): 60% suppression - Suppression of inflammatory cytokine (IL-1α) (UV irradiation): 50% suppression - Suppression of inflammatory cytokine (PGE2) (UV irradiation): 50% suppression =In Vivo= - Suppression of erythema (UV irradiation)

Krodesin is a nonapeptide (synthetic human nonapeptide-4) that mimics the active site of the anti-aging gene Klotho. The Klotho gene decreases with age and has been proven to have anti-aging effects. The name is derived from Klotho, the goddess in Greek mythology who spins the thread of fate. Klotho can protect the skin from the fate of aging and provide true rejuvenating effects. =In Vitro= Inhibition of insulin signaling (100%) Stimulation of SIRT1 gene expression (45%) Reduction of reactive oxygen species induced by peroxides (-10%) Inhibition of cellular aging Suppression of UV-induced inflammatory cytokine IL6 (-35%) =In Vivo= Anti-wrinkle (-50%) Fig.1 Elasticity (+200%), Pore size (-10%), number (-8%), Dermal papillae (+15%), luster (+20%), Sebum suppression (-10%)

BOREALG TM is a fat-soluble active substance derived from a large brown kelp called Laminaria hyperborea, known as the queen of longevity. Laminaria hyperborea inhabits the rocky surfaces of coastal areas. It has a strong adaptability to withstand the impact of powerful waves crashing against the reefs. In Brittany, for instance, when winter winds reach 130 km/h, the coastline is exposed to waves exceeding 10 meters. The pressure of the waves exerts 12 tons per square meter on the rocks and kelp forests. Odycea discovered that Laminaria hyperborea possesses ultimate restorative abilities to counteract strong waves. Borealg TG collected data focusing on the leptin receptor gene, fatty acid binding protein 3 gene, and Cyclin D1 gene, which are related to skin regeneration, activation, and rejuvenation processes. - Increase in leptin receptor gene expression stimulation: 139% improvement - Increase in fatty acid binding protein 3 gene expression stimulation: 220% improvement - Suppression of Cyclin D1 gene expression stimulation: -36% - Mitochondrial survival rate: 18% improvement - Suppression of erythema: -10%

MARINOVA is a biotechnology company established in 2003 in Tasmania, Australia. It is the manufacturer of the world's only high-purity organic fucoidan extract derived from brown algae, using its unique green chemistry technology. The company's product, MARITECH BRIGHT, is made from sustainable raw materials. =Sustainable Manufacturing Process= 1) Sustainable Sourcing: Wild seaweed is sourced from four regions around the world, employing harvesting methods that consider regeneration. 2) Zero Waste: Renewable energy (zero CO2 emissions) is used, and waste is utilized in agriculture. 3) Extraction with water, with 50% being reused. =About the Product= - Ingredients: Contains ≥90% fucoidan and polyphenols - Efficacy: 1) Anti-aging functions - Improves skin radiance - Reduces wrinkle depth - Stimulates expression of anti-aging gene SIRT1 - Inhibits glycation (50%) - Suppresses collagenase, tyrosinase, and elastase by up to 99% 2) Whitening functions - Improves pigmentation (20%) 3) Antioxidant functions - Removes free radicals - Against UVA/B: Suppresses erythema and TEWL caused by irradiation - Oxygen radical absorption capacity (twice that of VC)

- Ingredients: Contains Fucoidan ≧ 85% - Efficacy: 1) Anti-aging function - Wrinkle depth: -9% - Stimulation of anti-aging gene SIRT1 expression (+28%) - Glycation inhibition effect (-50%) - Inhibition of collagenase and elastase by up to 99% 2) Protection effect - Suppression of erythema after UV exposure by 45% - Antiviral effect (multiple including herpes simplex) 3) Moisturizing and smoothing - Increase in moisture by 30% after UV exposure - Increase in stimulation of skin cell expression after UV exposure - Inhibition of myeloperoxidase by up to 99%

HyRetin is a product that introduces retinoids to low molecular weight sodium hyaluronate, enhancing the stability and skin permeability of retinoic acid. =In Vitro= - Anti-inflammatory effects: IL-8: -15%, IL-6: -40% - Promotion of collagen type 1 production: +40% - Promotion of fibronectin production: 1.4 times (vs control) =In Vivo= - Dermal density: +10% improvement - Number of dermal papilla cells: +48% - Improvement in skin elasticity: -34% (recovery time) - Reduction in wrinkle depth: -36% - Skin radiance: +43% - Skin moisture level: +7% - Erythema suppression effect: -6% - Sebum reduction effect: -36% - Pore size: -25% - Anti-acne effect: size: -50%, number: -68.75% and more.

The Baobab (scientific name: Adansonia) is a deciduous tree native to regions such as Africa and Madagascar, belonging to the Malvaceae family and the Baobab genus. In Africa, its fruit has been consumed as a panacea since ancient times and is referred to as the "Tree of Life." The trunk of the Baobab tree has a shape resembling a sake bottle, is hollow inside, and can store up to 10 tons of moisture in its trunk, allowing it to survive by shedding its leaves and entering dormancy during the dry season, relying on the moisture stored within its trunk. LIFTISSTM is an extract obtained from the fruit of this Tree of Life, and it is a gel-like product rich in organic acids (AHA) and natural moisturizing factors (NMF), containing natural sugar components (77% galacturonic acid). <Mechanism of Action> Organic acids (alpha-hydroxy acids) break down keratin on the skin's surface, promoting exfoliation and epidermal regeneration, resulting in smoother skin and providing moisturizing and wrinkle-smoothing effects. Natural sugars (components of NMF), such as glucose and xylose, bind with keratin to form complexes that attract water molecules, thereby providing moisturizing effects. Additionally, natural sugars form a film on the epidermis, creating a tightening effect. <Efficacy> - Number of wrinkles: -41% - Area of wrinkles: -24% - Lifting effect, etc.

This product is an extract derived from the new buds of linden using GREENTECH's patented cryo-extraction technology. Linden is a long-lived tree that can live for centuries and was considered a sacred tree in ancient Germany. The extract of its flowers has anti-inflammatory and protective properties and has been used for various purposes. In particular, the new buds consist of embryonic tissue from the proliferation process, are full of vitality, and are rich in active components necessary for growth, such as plant hormones, proteins, amino acids, and sugars. The company's patented technology allows for the preservation and extraction of the active components of the new buds without damage. <Mechanism> It forms a film on the surface of the hair, suppressing moisture release, protecting the cuticle, smoothing the hair surface, and making it less prone to tangling. Additionally, by simultaneously providing flavonoids, the helical bonds of keratin (the fibrous protein that makes up 95% of hair) are stabilized. Furthermore, using free sugars (components of natural moisturizing factors) forms a water complex in the upper layer of the scalp, providing constant hydration. <Efficacy> - Improvement in hair moisture levels: +83% - Improvement in scalp moisture levels: +33% - Cuticle improvement - Volume enhancement - Static electricity prevention

The structure of hair fibers is the most important factor for the quality and health of hair. When the fiber structure deteriorates, the shine and smoothness of the hair decrease. Hair is constantly exposed to external stress factors such as sunlight and pollutants. These stress factors are referred to as "exposome." The structure of hair fibers deteriorates due to the exposome, leading to a decrease in the mechanical strength, shine, and smoothness of the hair. ZORYALYS is a raw material composed of three extracts developed to protect hair from the exposome. The extracts used are derived from ginger root, magnolia bark, and placasia, each serving a specific role: ginger provides antioxidant functions, magnolia offers protection against light damage, and placasia oil contains components similar to sebum in hair, improving hair quality. In Vivo (Caucasian, Asian) - Cortex protection against UV-A, cuticle protection, overall hair protection (evaluation of carbonylation) - Protection of keratin fiber structure against xenon (sunlight), prevention of tryptophan (melanin precursor) decomposition, prevention of cysteine participation, maintenance of mechanical strength, improvement of shine (twice as much), prevention of hair color fading.

Alopecia is a common disorder caused by the miniaturization of hair follicles, leading to the softening of hair. The most common type of hair loss is androgenetic alopecia, which accounts for 90% of cases, with 28% of men aged 18-29 and 53% of men over 40 being affected by this condition. From a pathophysiological perspective, the causes of alopecia are multifactorial, including stress, nutrition, exposome, genetic predisposition, and hormonal sensitivity. The scalp microbiome has a significant relationship with hair growth disorders. Finding the optimal balance of both bacterial and fungal communities in hair is a step towards maintaining healthy hair. GREENTECH has developed anti-hair loss ingredients aimed at normalizing the scalp microbiome and regenerating follicular cells, focusing on the Wnt pathway, which plays a key role in the hair follicle and hair growth cycle. In Vitro 1) Inhibition of key signaling pathways for hair growth: - DDK-1 inhibition: 89% - TGFβ1 inhibition: 55% - sFRP-1 inhibition: 21% - Endothelin-1 inhibition: 21% 2) Increased proliferation of dermal papilla cells: 41% increase (better than minoxidil) 3) Adjustment of the microbiome In Vivo Hair density: 28.5% improvement

Aging and pregnancy increase the skin's sensitivity to sunlight, leading to excessive pigmentation and the formation of spots and melasma. The three types of plant extracts included in this product approach spots in three different ways, thereby improving spots and melasma, as well as enhancing skin uniformity and brightness. <Three Approaches> - Mulberry bark extract: Suppresses melanin production through tyrosinase inhibition - Hibiscus flower extract: Promotes turnover by exfoliating dead skin cells - Cucumber seed extract: Inhibits the transfer of melanin to epidermal cells <Efficacy> In Vitro: - Tyrosinase inhibition effect: -18% - Melanin production inhibition: -22% - Melanin transfer inhibition: -22% In Vivo: Brightness improvement: 1.5 times increase Improvement of pigmentation: 6.5 times improvement

BIOTILYS enhances the expression of antimicrobial peptides, improving antibacterial activity, anti-inflammatory effects, barrier function recovery, and the diversity of the skin microbiome. <Effects of Antimicrobial Peptides> - Antimicrobial peptides have a broad range of antibacterial activity and have been reported to exhibit antibacterial activity against Gram-positive Staphylococcus aureus, Gram-negative Pseudomonas aeruginosa, and even drug-resistant bacteria. - They have also been confirmed to improve tight junction barrier function and have anti-inflammatory effects on the skin, showing promise for the treatment of atopic dermatitis and psoriasis. <Stimuli for Antimicrobial Peptide Expression> - β-defensin 2 (hBD2): +229% - β-defensin 3 (hBD3): +19% - S100A7 (soriacin): +237% <Anti-inflammatory> - Toll-like receptor 2 (TLR2): -57% - IL-8: -58% <In Vivo: Barrier Function Recovery Effects> - Water vapor loss: -31% (after 6 hours) - Moisturizing function: +27% (after 3 hours) - Improvement in the diversity of the skin microbiome

Exposure of the skin to UV, BL, and IR can lead to the formation of free radicals and inflammatory responses, resulting in apoptosis and the breakdown of the extracellular matrix (ECM). These can cause short-term issues such as sunburn, dryness, sensitive skin, and erythema, while long-term effects may include dark spots, uneven skin tone, and photoaging. Sorberlin focuses on the flowers of the Japanese bush clover, which are rich in phenylpropanoids that absorb UV, BL, and IR, particularly standardizing high contents of verbascoside and echinacoside, which have strong light-absorbing effects. - UV irradiation: Inhibition of lipid oxidation: 54% Inhibition of isomerization of urocanic acid: 70% Inhibition of apoptosis: 57% Inhibition of sunburn cells: 40% Inhibition of MMP-1: 30% Promotion of elastin production: 7% Inhibition of TNF-α: 36% Inhibition of melanin: 62% - BL irradiation: Inhibition of ROS production: 43% Inhibition of TGF-β1: 88% Promotion of decorin production: 29% Reduction of aging spots: 81% - Under IRA irradiation: Inhibition of MMP-1: 22% Inhibition of IL-6: 27% Inhibition of TGF-β1: 35% - In Vivo: Inhibition of erythema (L value): 33% ITA value: 189%

In 2020, the Covid-19 pandemic redefined our communication styles. The lockdown created distance between us and our social lives, forcing us to change and adapt the way we engage with society and understand the state of the world. Additionally, protecting ourselves from the virus became our primary concern, leading to a life where wearing masks was mandatory. Wearing masks causes an increase in temperature and humidity inside the mask, resulting in various adverse effects such as erythema, itching, dryness, seborrheic dermatitis, and impetigo. 1) EXPOZEN: Microbiome ingredient for sensitive skin - Suppression of the TRPV-1 receptor, which causes stinging, inflammation, and redness - Inhibition of the redness-causing bacteria C. kroppenstedtii - Erythema suppression effect 2) ACNILYS: Microbiome ingredient for acne - Inhibition of the acne-causing bacteria C. acnes IA1 - Suppression of the bacteria C. granulosum that proliferate in acne 3) BIOTILYS: Microbiome ingredient for enhancing barrier function - Promotion of antimicrobial peptide production - Improvement of barrier function - Enhancement of skin microbiome diversity 4) POLEVAN S: Sugars for restoring barrier function and dry skin - Increased resistance to osmotic shock

Human skin is a complex and diverse ecosystem. It is home to not only human skin cells but also bacteria, fungi, viruses, and mites, making it one of the very complex ecosystems that are still not well understood. When the balance of the skin microbiome is disrupted, it can affect skin health. Changes in resident skin bacteria have been confirmed to be related to skin concerns (such as acne, dryness, redness, hair loss, and dandruff) that are focused on in skincare and haircare. Green Tech Company believes that cosmetics targeting the microbiome could become one of the effects that consumers will prioritize in the future, and they are developing various products. <Product Information> - EXPOZEN: Reduces the stinging sensation and redness of sensitive skin, anti-wrinkle effect - BIOTILYS: Promotes the production of antimicrobial peptides, suppresses harmful bacteria, enhances the diversity of symbiotic bacteria, restores barrier function - ACNILYS: Suppresses acne-causing bacteria IA1 (maintains the balance of symbiotic bacteria), improves acne, reduces sebum, inhibits 5-alpha reductase - DANDRILYS: Improves dandruff, alleviates itching, reduces sebum, suppresses bacteria related to sebum secretion, improves the balance of resident bacteria - HAIRILINE: Suppresses bacteria related to hair loss, restores bacterial balance, promotes hair growth

Today, it is said that 50% of women in Europe, America, and Japan have sensitive skin. Regardless of the presence of erythema, sensitive skin experiences excessive reactions to environmental factors, accompanied by sensations such as stinging and itching. These factors can be external (such as UV rays and chemicals) or internal (such as stress and hormones), and in most cases, they cause discomfort to the skin and visible symptoms. Additionally, it is believed that skin microbiota are involved in the symptoms of sensitive skin. In response to this significant challenge, GREENTECH has developed a functional product, ExpoZen, to protect the skin from harmful exposomes. In Vitro: Effects on inflammation and neurogenic inflammation suppression: - TRPV-1: -67% - TNF-α: -21% - NK1-R: -43% - VEGF: -16% Effects on barrier function improvement: - Increased epidermal thickness: +12% In Vivo: Microbiome regulation: - Suppression of bacteria associated with inflammation and redness (C. Kroppenstedtii) - Increase of beneficial bacteria (S. epidermis) Improvement of sensitive skin symptoms: - Skin irritation: -34% - Pain: -33% - Tingling: -40% - Erythema: -24%

It is said that 85% of individuals aged 12 to 24 struggle with oily and acne-prone skin, and this trend is increasing in older age groups as well. The main causes of acne are (1) excessive sebum production, (2) irregular keratinization, (3) inflammation, and (4) sebaceous gland involvement. These causes lead to inflammatory responses. Furthermore, clogged pores create a low-oxygen environment that facilitates the proliferation of acne bacteria. Whether acne bacteria act symbiotically or worsen acne symptoms depends on the balance of their strain types. There are mainly six strain types of acne bacteria: IA1, IA2, IB, IC, II, and III. It has been confirmed that strain type IA1 is the dominant type in acne-prone skin. This product suppresses the IA1 strain of acne bacteria and the C. granulosum that increases within papules, maintaining and improving the balance of symbiotic bacteria. In Vitro: - Sebum suppression effect: - Inhibition of 5-alpha reductase - Inhibition of acne bacteria IA1 - Anti-inflammatory action In Vivo: - Maintenance of major pore balance - Suppression of C. granulosum - Improvement of acne - Brightness improvement - Uniformity improvement

- Circadian Rhythm Circadian rhythm is a physiological phenomenon that regulates certain processes in the body on an approximately 25-hour cycle. Most processes in the body, including wakefulness rhythm, autonomic nervous system, endocrine hormone system, immune system, and metabolic system, are regulated by circadian rhythms. Disruptions in these rhythms are correlated with numerous diseases (obesity, cancer, new vascular diseases, diabetes, sleep disorders, etc.) and skin conditions (dark circles, wrinkles, fatigue, etc.). - Circadian Rhythm Monitor Cells This product is a monitor cell developed to find components that regulate circadian rhythms. It is a mouse embryonic fibroblast that can measure the expression rhythm of the bmal1 gene, one of the clock genes that form the body clock. It is derived from a transgenic mouse (Bmal1-ELuc mouse) created using a construct that links the luminescent protein Eluc downstream of the bmal1 gene promoter, prepared from mouse embryos. - Details Cell Name: Circadian Rhythm Evaluation Cells (Bmal1-Eluc MEF) Origin: Mouse Cell Count: 5.0×10^5 cells/vial Storage Method: Liquid nitrogen tank Ultra-low temperature freezer (-140°C) Deep freezer (-80°C)

Since the population of the silver generation (aged 65 and over) surpassed that of the population aged 5 and under in 2020, the number of people in the silver generation has been increasing year by year. In particular, the proportion of women in the silver generation is expected to reach 13.4% by 2030. Modern silver generation women tend to prefer aging gracefully, and "Heberis" has been developed as an ingredient for positive aging. This product is a fermented extract from a bacterium called Sphingomonas, which can survive in extreme environments. It contains various active ingredients, including polar lipids, that have a zombie cell effect. ■In Vitro - Induction suppression of zombie cells: p21: -58%, p16: -20% - Suppression of zombie cells: -78% (β-galactosidase) - Suppression of SASP: IL-8: -32%, IL-6: -33% - Recovery of extracellular matrix: collagen production: +&%, fibrin: +22%, proteoglycan: +7% ■In Vivo - Depth of crow's feet wrinkles: -8% - Skin roughness: +14% - Elasticity: +12% - Tone (sensory evaluation): brightness: 74%, uniformity: 83% - Psychological aspect: self-esteem: +10.5%, mood state: +9%

Aqua Sil Oil is a self-emulsifying amino acid-based surfactant created by reacting lysine with the fatty acids of plant oils. There are six grades available, using six types of plant oils (argan oil, Inca inchi oil, sweet almond oil, apricot oil, olive oil, and sunflower oil). <Features> - Due to the differences in the fatty acid composition of the six types of plant oils, properties such as surface tension, critical micelle concentration, foaming ability, foam stability, and cleansing power vary. - It decomposes at a low pH, so the displayed name is separated into plant oil and lysine. It can be used as surfactant-free according to the displayed name. - Biodegradable - Self-emulsifying <Efficacy> - Moisturizing effect - Inhibition of Staphylococcus aureus and promotion of Staphylococcus epidermidis - Antioxidant effect <Applications> - Aqueous cleansers - Masks - Surfactant-free emulsification formulations - Utilization in formulations where adding plant oils is difficult (cleansers, shampoos, wipe-off lotions, etc.)

- ARCOLYS: Anti-gray hair ingredient - HAIRILINE: Anti-hair loss ingredient, scalp microbiome regulation - DANDRILYS: Anti-dandruff ingredient, scalp microbiome regulation, sebum control - ZORYALYS: Anti-pollution, anti-UV, IRA effect ingredient - TILICINE: Cuticle improvement effect, frizz control effect, scalp care ingredient - INCA INCHI OIL: Dry oil containing 45% omega-3, hair repair effect ingredient - BAOBAB OIL: Sustainable dry oil rich in omega-6 and omega-9 - APRICOT OIL: Oil rich in omega-6 and omega-9 - HAIR STRENGTH EGX292: Scalp care extract mixed with five types of extracts - COMPLEX HAIR LUSTRE: Hibiscus flower extract that softens hair and enhances shine and texture

Hawthorn Cosmelean is an extract from the flowers of the Western Hawthorn. It contains an active ingredient, Vitexin-2-O-rhamnoside, at a concentration of 0.07-0.11%. <Mechanism of Action> - The flavonoids contained in Cosmelean Hawthorn reduce congestion in capillaries and alleviate erythema. - By promoting blood flow, it enhances the oxygen levels in the skin, activating a constant process. <Efficacy> Reduces redness in the skin and brightens it. Unifies skin tone and improves transparency. Reduces skin discomfort/unpleasant sensations (tightness due to dryness, inflammation). In Vivo: Redness reduction (IR-induced): -6% Redness reduction (UV-induced): -7%

Chamomile Cosme Ren is a plant extract containing 0.01-0.02% apigenin as an active ingredient derived from chamomile flower extract. <Mechanism of Action> It suppresses the production and release of inflammatory factors that cause redness. It alleviates skin tingling and brings healing. <Efficacy> Soothes the skin and suppresses redness. Relieves tingling sensations. Reduces eye congestion. In Vivo: Redness suppression: -26% Reduction of UV-induced skin temperature increase: -0.2℃

Ivy Cosme Ren is an extract derived from the stems and leaves of ivy, containing: Hedera coside: 0.4-0.6%, Saponins: 2.5-6.0%, Flavonoid glycosides, Phenolic acids, and Polyacetylenes. Cosmetic ingredient name: Hedera helix leaf/stem extract <Mechanism of action> It exerts a drainage effect (blood flow promotion effect) through two pathways: (1) Microcirculation promotion effect due to relaxation (2) Adjustment of capillary permeability through anti-hyaluronidase effect <Efficacy> Removes cellulite and improves skin unevenness Reduces the appearance of dark circles under the eyes, providing a more relaxed expression In Vitro: Blood flow promotion effect in microvessels: +4% (3 hours after application), +14% (applied twice a day for 14 days)

Ginseng Cosmelean is an extract derived from the root of ginseng, which is used as a tonic and activates the body's functions as a traditional herbal medicine. It contains ginsenosides as its main component. This product is expected to promote collagen production in skin cells, strengthen the extracellular matrix, enhance barrier function, and improve firmness and elasticity. <Mechanism of Action> Ginseng Cosmelean promotes collagen production in skin cells, strengthens the extracellular matrix, and improves elasticity. This product is expected to enhance barrier function and improve firmness and elasticity. *The multifunctional medicinal effects of ginseng have been well known since ancient times. Among these, its anti-aging properties have also been reported, particularly its palliative effects in elderly patients with terminal illnesses. <Efficacy> Improves skin elasticity and enhances firmness. Makes the skin appear youthful. In Vivo: Firmness improvement: up to +9% Elasticity improvement: up to +22%

Centella Asiatica Cosmelean contains triterpenes such as asiaticoside and madecassoside, and is a standardized product. It activates collagen synthesis by fibroblasts and maintains the structure and function of the skin. Triterpenes (asiaticoside and madecassoside) exhibit nutritional and regulatory effects on connective tissue, particularly enriching and regulating fibroblasts and glycosaminoglycans. They promote the synthesis of collagen I and III, enhance keratinization, and accelerate the healing process. In vitro studies have reported that asiaticoside and madecassoside improve the secretion of collagen I, while madecassoside promotes the synthesis of collagen III. In vivo: • Number of moderate wrinkles: -42% • Depth of moderate wrinkles: -3% • Number of deep wrinkles: -29% • Total area of wrinkles: -30%

This product is an extract derived from the black truffle of the Tuber melanosporum species. Black truffles are known as a luxury ingredient. Their rich and robust aroma and delicate flavor are highly valued, earning them the nickname "black diamonds." Truffles produce a wide variety of compounds during their maturation period, with over 50 aroma-related compounds discovered and collected by animals and others. Due to their abundant active ingredients, truffles have been used in traditional medicine, and research is currently being conducted on their anti-cancer effects and treatment for Alzheimer's disease. <Active Ingredients> - Oligosaccharides, polysaccharides - Phenolic compounds - Phosphorus, potassium - Amino acids, proteins <Efficacy> Antioxidant effects (Villares et al., 2012): Inhibition of lipid oxidation Protection effects (Zampieri et al., 2011): Increased stimulation of various heat shock protein genes Moisturizing effects (Martini and Seiller, 2006): Cell activation effects (Martini and Seiller, 2006):

- Argan oil cake (oil residue): Argania spinosa extract - Off-spec melon fruit: Melon fruit extract (moisturizing effect) - Off-spec lime fruit: Lime fruit extract - Off-spec cucumber fruit: Cucumber fruit extract - Beehive: Propolis extract - Byproduct of spice manufacturing: Saffron flower extract - Byproduct of olive oil production: Olive fruit extract - Byproduct of tea leaf screening: Tea leaf extract - Byproduct of baobab seed oil production (baobab pulp): Baobab pulp extract (moisturizing effect, lifting effect)

This product is an extract derived from ferns native to Polynesia. Polynesian ferns are rich in ecdysteroid, a miraculous anti-aging hormone. This plant hormone is also found in insects and is crucial for their metamorphosis. The hormone that induces radical morphological changes in insects fundamentally improves the structure of the skin when applied topically. Mechanism of Action: - Promotes the production of structural proteins at the dermal-epidermal junction, strengthening the cohesion between the dermis and epidermis, thereby improving skin elasticity. - Stimulates epidermal regeneration by promoting the proliferation and differentiation of basal cells. - The epidermis quickly becomes layered and compacted, restoring the voluminous appearance of youthful skin. - The stratum corneum regains complete cohesion and functions as a barrier once again. In Vitro: - Ki67 (cell cycle (cell proliferation promotion)): 3.3 times - Basement membrane constituent proteins: Involucrin: 5.3 times Type IV collagen: +22% Laminin 5: +12% Fibrin: +25% - Dermal thickness: 2.3 times In Vivo: - Elasticity improvement: 3.5 times - Moisture levels: 2.5 times - Skin isotropy: +16%

Green Tech Co. confirmed the inhibitory effect on genes that protect the extracellular matrix and suppress cellular aging using a seaweed extract rich in glucuronic acid oligosaccharides against 44,000 human genes with DNA microarrays, and developed QT40. This product is a raw material that provides a lifting effect by inhibiting zombie cells and promoting extracellular matrix structural proteins. In Vitro: FGF1 gene expression stimulation: +35% CCNA2 gene expression stimulation: +80% *CCNA2 has a function that promotes cell cycle progression as a DNA repair and p21 inhibitor, and is expected to prevent cellular aging and the emergence of zombie cells. Collagen synthesis: +22% Hyaluronic acid synthesis: +17% MMP1: -29% MMP3: -31% In Vivo: Improvement of sagging: 2.8 times improvement V-line tightening: Over 60% of subjects experienced it Improvement of elasticity: +20.5%

The skin is a barrier between the internal body and the external environment. The epidermis, which is exposed to the external environment, is home to a diverse microbiome. This microbiome includes bacteria that metabolize components secreted in sweat and sebum, converting them into volatile substances. These volatile substances are the cause of body odor. The repeated use of deodorant products with antibacterial effects indiscriminately kills skin resident bacteria, posing a risk of damaging the skin's natural microbiome. Therefore, GREENTECH has focused on the antibacterial properties of the lactic acid bacterium Lactococcus lactis and developed LACTOPHYT, a component that selectively kills odor-causing bacteria. =Effects= Suppresses odor-causing bacteria Maintains beneficial bacteria Reduces axillary odor =In Vitro= <Commensal Bacteria> Staphylococcus epidermidis: 24h–72h: + <Odor-Causing Bacteria> S. HOMINIS: 24h–48h: - C. XEROSIS: 24h–72h: - C. TUBERCULOSTEARICUM: 24h–72h: - *S.: Staphylococcus, C.: Corynebacterium =In Vivo= Body odor intensity: -30% (Day 1), -35% (Day 3)

KIGELINE(R)/Kigerin is an extract from the fruit of Kigelia africana (sausage tree), used as a traditional African breast enhancement agent. This product is the best-selling item from Green Tech in France. <Main Ingredients> Saponins, Phytosterols, Flavonoids <Mechanism of Action> Saponins and phytosterols act on dermal fibroblasts, promoting the synthesis of hyaluronic acid and collagen. The increase in these extracellular matrix components contributes to improving skin firmness and elasticity, as well as increasing skin moisture retention, thereby enhancing the firmness and fullness of the breasts. Flavonoids exhibit anti-aging effects by promoting blood circulation. The additive effects of these three components provide firmness and elasticity to the breasts, resulting in a lift. <Efficacy> Breast enhancement effect: Lifts the breasts by improving skin elasticity and moisture retention. Anti-aging effect: Beautifies the neck line through improved firmness. <In Vivo> Accuracy measurement by iconography - Breast enhancement: +70% - Lift: +18%

Timiline is a polyglucuronic acid obtained by purifying bacterial fermentation products and chemically modifying them. Greentech developed Timiline as a cellulite removal ingredient based on transcriptome analysis using DNA chips, screening 44,000 human genes and finding that Timiline promotes the expression of the FIAF adipokine gene. <Mechanism of Action> TIMILINE targets four factors to remove cellulite: - It inhibits LPL to reduce lipid biosynthesis and fat accumulation. - It stimulates ATGL to break down accumulated fat and release it extracellularly. - It promotes the transport of fatty acids from subcutaneous tissue to peripheral tissue. - It suppresses the differentiation of preadipocytes into mature adipocytes. <Efficacy> In Vitro - Suppression of intracellular lipids: -26% - Inhibition of differentiation of preadipocytes into mature adipocytes - Promotion of production: collagen +30%, integrin +17%, glycosaminoglycan +17% - Promotion of integrin production: In Vivo - Cellulite removal: improvement in 64% of subjects - Thigh circumference: approximately 67% reduction - Improvement in elasticity: 67% reported feeling it - Improvement in dimpling: 53% reported feeling it