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Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 3

Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 3

Introduction of the use of immunoglobulins for pharmaceutical and diagnostic drug development in an article.

This article introduces the use of immunoglobulins for drug and diagnostic development. It includes topics such as "Use as Human Antibody Reference and Positive Control," "Chimeric Surrogate Antibodies," "Pharmacokinetic Analysis Tools," and "Human IgG Standard Samples for Immunogenicity Testing." Please take a moment to read it. [Contents] ■ Use as Human Antibody Reference and Positive Control - IgA for HuCAL reference samples in anti-phospholipid antibody syndrome ELISA ■ Chimeric Surrogate Antibodies ■ Pharmacokinetic Analysis Tools - High-sensitivity pharmacokinetic assay systems using HuCAL antibodies for drug discovery ■ Human IgG Standard Samples for Immunogenicity Testing - HuCAL IgG1 standard samples in homogeneous bridging assays *For more details, you can view the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 4

Introduction to Custom Monoclonal Antibody Production Service Case Studies vol. 4

Utilizing the features of a unique antibody production technology that does not rely on animals! An article introducing strategic selection.

The monoclonal antibody production service by Antibody Craftsman utilizes a unique antibody production technology that does not rely on animals, allowing for in vitro selection to narrow down the regions of antigens that antibodies bind to and to create antibodies that can distinguish slight differences in antigens. Here, we introduce some of the selection methods used to obtain the desired Fab antibodies. Additionally, please take a look at our achievements in antibody production. [Content Included] ■ Antibodies that identify post-translational modifications ■ Antibodies that specifically recognize homologous regions ■ Antibodies against membrane proteins ■ Antibodies that distinguish amino acid substitutions and deletions ■ Antibodies that recognize three-dimensional structures ■ Antibodies with strict specificity *For detailed content, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 5

Introduction to Custom Monoclonal Antibody Production Service Case Studies Vol. 5

Deliberately selecting antibodies that are recognized by autotaxin! An article introducing examples of antibodies that inhibit activity.

We will introduce a case of obtaining antibodies that inhibit activity, which could not be achieved through animal immunization, using the phage display method called "Antibody Craftsman," specifically in the case of autotaxin. The reason why inhibitory antibodies against autotaxin (ATX) could not be obtained through animal immunization is thought to be due to the high conservation of ATX across species, making it difficult to establish inhibitory antibodies targeting the catalytic active site. Therefore, in the Antibody Craftsman approach, we intentionally selected antibodies that recognize both human and mouse autotaxin (ATX) and developed a strategy to select inhibitory antibodies from among them. 【Contents】 1. What is Autotaxin 2. Structure of Autotaxin 3. Strategic Screening - 3-1. Panning - 3-2. ELSIA Screening - 3-3. Enzyme Activity Inhibition Screening 4. Activity Measurement Using Antibodies 5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 6

Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 6

Selection of antibodies that bind only to mutant types! An article introducing the production of antibodies that distinguish between mutant types differing by just one amino acid.

In cases where a protein with a single amino acid mutation exists in a mixed state alongside the wild-type sequence, there may be a need for antibodies that can specifically distinguish this mutated protein. Specifically, as an antigen, a peptide synthesized from the mutated sequence portion is used, and through panning via phage display and ELISA screening, clones that are negative for the wild-type and positive for the peptide with the single amino acid mutation are selected. I will introduce each step. [Contents] 1. Design and coupling of peptide antigens 2. Panning 3. ELISA screening 4. Sequencing 5. Selection of candidate antibodies *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 7

Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 7

Setting the antigens is very important! An article introducing the selection of antibodies that distinguish the differences at the terminal ends.

When proteins undergo processing and are divided into multiple fragments, it becomes very important to set the antigens for producing antibodies that recognize each fragment. Antibody artisans perform all selections in vitro without using animal immunization, allowing them to conduct binding reactions with peptides or proteins in their prepared state, without the steps of fragmenting proteins for antigen presentation. In this article, we will introduce the antigen setting and selection methods for producing antibodies that can distinguish between fragments before and after cleavage by a protease of a certain protein. 【Contents】 ■1. Specificity of antibodies ■2. Design of antigens ■3. Screening methods 　・3-1. Panning 　・3-2. ELISA screening ■4. Protein antigens and peptide antigens 　・4-1. Which one should be chosen? 　・4-2. Innovations in selection ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 11

Introduction to Custom Monoclonal Antibody Production Service Case Study Vol. 11

It is possible to narrow down the field! An article introducing antibodies that distinguish phosphorylated Ser.

Antibody artisans conduct selection in vitro against fixed antigens without using animal immunity. This allows for binding reactions to be performed in the state or environment of prepared peptides or proteins, enabling precise antigen design, such as narrowing down the area of interest. Here, we present an example of antibody production using peptide antigens to obtain antibodies that recognize specifically phosphorylated proteins at certain Ser residues. We invite you to read on. [Contents] 1. Preparation of Antigens 2. Selection to Increase Specificity 3. Selection of Antibodies 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

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Monoclonal Antibody Production Contract Service "Antibody Craftsman"

Monoclonal Antibody Production Contract Service "Antibody Craftsman"

Success-based reward! We will deliver antibodies that distinguish slight differences in 7 weeks.

Are you having trouble with the low specificity of conventional monoclonal antibodies? With "Antibody Artisan," you can produce antibodies targeting specific regions, resulting in antibodies with higher specificity. For example, we can produce antibodies such as: ■ Antibodies that identify post-translational modifications ■ Antibodies that specifically recognize homologous regions ■ Antibodies that distinguish between a few amino acid substitutions or deletions ■ Antibodies that recognize denatured proteins "Antibody Artisan" employs a unique technology (a library of 45 billion types of artificial human antibodies and phage display) that does not use animal immunization, allowing us to design antigens and select strategically. All work is done in vitro, enabling us to deliver antibodies as quickly as seven weeks after receiving the antigen. Additionally, the antibodies obtained through this service can be expressed and purified as recombinant proteins in E. coli, allowing for repeated production of additional antibodies. Antibody clones are stored for five years, and sequences are kept permanently. *For more details, please refer to the PDF materials or feel free to contact us.

  • Research Antibodies
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  • Clonal Antibody Production Service

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Antibody Production Contract Services_Abwiz Bio/East Japan Pharmaceutical Industry

Antibody Production Contract Services_Abwiz Bio/East Japan Pharmaceutical Industry

We offer custom antibody production services, antibody affinity enhancement services, and various antibody products!

Kyoto Pharmaceutical Industry Co., Ltd. offers antibody services from the American company Abwiz Bio. 【Antibody Services】 ■ Custom antibody production service (rabbit monoclonal antibodies) ■ Affinity enhancement service ■ Rabbit monoclonal antibody products 【Features of Monoclonal Antibody Production】 ■ Using Abwiz Bio's proprietary antibody gene cloning method WizAmpTM (patented in the U.S.), we can produce antibodies with high specificity and high affinity. ■ By combining a vector specialized for rabbit antibody expression with WizAmpTM, we have successfully obtained numerous antibody clones that were difficult to acquire with conventional phage vectors. 【Features of Affinity Enhancement Service】 ■ Abwiz Bio's unique three-step affinity enhancement platform improves antibody affinity. ■ Achieves over tenfold affinity enhancement. ■ Allows for selection according to various applications through biopanning.

  • Research Antibodies
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  • Cosmetic materials and raw materials
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Ibeck Corporation Monoclonal Antibody Production Service

Ibeck Corporation Monoclonal Antibody Production Service

We will assist in the practical application of drug discovery seeds and the development of pharmaceutical candidate antibodies.

We use human PBMCs (peripheral blood lymphocytes) as materials to clone the target antibody gene using the EBV method and produce fully human monoclonal antibodies. We create a library of healthy human samples consisting of over 100 specimens to assess the likelihood of obtaining the target antibody from healthy individuals. If there are specimens from which we can determine a high probability of acquiring the target antibody based on the results obtained, we can start antibody screening using our owned sample specimens. We emphasize acquiring antibodies that maintain their original activity, producing full-length heavy and light chains without converting them to scFv. 【Features】 - Application of the EBV method - Cloning of the target antibody gene and production of fully human monoclonal antibodies - Emphasis on acquiring antibodies that maintain their original activity - No scFv conversion *For more details, please refer to the catalog or feel free to contact us.

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Monoclonal Antibody Production Contract Service: Technical Information 2

Monoclonal Antibody Production Contract Service: Technical Information 2

Unique Antibody Production Technology - HuCAL Technology

HuCAL technology is an antibody production technique that does not rely on animals and conducts all selections in vitro. Specifically, antibodies are presented using phages that incorporate human antibody genes, allowing them to bind to antigens. Since the presented antibodies (Fab antibodies) correspond one-to-one with the genes that code for them in the form of phage particles, it is easy to identify the genes of the Fab antibodies that bind to the antigen from among many Fab antibodies (phage display). A solution containing phages is added to a plate with immobilized antigens, allowing the phages to bind through the presented antibodies. Phages that did not bind to the antigens are washed away, and the bound phages are then eluted and collected. By repeating this process several times, phages that bind more strongly to the antigen can be concentrated (panning). The antibody genes encoded by the phages obtained through panning are expressed in E. coli, and Fab antibodies are purified. *For more details, please refer to the PDF materials or feel free to contact us.*

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Monoclonal Antibody Production Contract Service Price

Monoclonal Antibody Production Contract Service Price

Additional production of Fab antibodies (after the completion of antibody production)

The candidate antibodies and final antibody clones produced by the antibody craftsman will be stored for 5 years (extendable). We will accept additional production of the selected antibodies at the following prices (from mg to g orders). Antibody production setup Desired selling price for His-tag antibody: ¥80,000 Desired selling price for Strep-tag antibody: ¥114,000 Per 1 mg of antibody production Desired selling price for His-tag antibody: ¥70,000 Desired selling price for Strep-tag antibody: ¥70,000 *For more details, please refer to the PDF document or feel free to contact us.

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Monoclonal Antibody Production Contract Service Q&A

Monoclonal Antibody Production Contract Service Q&A

Antibody Artisan Frequently Asked Questions Part 2: What is the difference from other phage display methods?

Question: What is the difference from other phage display methods? Answer: The concept and methods for creating the library are different. We analyze the structure and sequences of human antibodies and construct a high-quality library artificially synthesized from DNA according to the rules of human antibodies. *For more detailed information about the service, please refer to the PDF document or feel free to contact us.*

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【Feature 2】Seven characteristics of monoclonal antibody production contract services.

【Feature 2】Seven characteristics of monoclonal antibody production contract services.

The success rate over the past three years boasts over 90% and has been adopted by many pharmaceutical companies.

For features 1 to 3, please refer to the seven characteristics of our monoclonal antibody production contract service. 4. Customization through molecular engineering It is possible to add various tags to antibodies or produce various fusion proteins. Additionally, dimerization can be achieved using a helix-turn-helix motif, which can enhance antigen cross-linking ability and improve affinity. 5. Abundant options As an option, we can also handle the preparation of antigens. Conversions to human IgG, IgA, or mouse chimeric IgG, as well as affinity enhancement after antibody production, increasing the number of candidate antibodies, and additional antibody preparations are possible. 6. No cost if ELISA-positive antibodies are not obtained Our service boasts a success rate of over 90%. Based on this confidence backed by our success rate, there will be no charge if ELISA-positive antibody clones are not obtained against the antigen. 7. Continuous supply of antibodies Antibodies are backed up in three formats: whole cells, plasmid DNA, and DNA sequence information, ensuring that all antibodies can be rapidly reproduced from these backups, guaranteeing a continuous supply. *Please feel free to contact us for more details.

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Technical information about the "Monoclonal Antibody Production Contract Service."

Technical information about the "Monoclonal Antibody Production Contract Service."

Unique Antibody Production Technology - HuCAL Technology

In the antibody artisan's monoclonal antibody production service, we produce monoclonal antibodies using the HuCAL technology developed by MorphoSys and owned by Bio-Rad (formerly AbD Serotec, A Bio-Rad Company). HuCAL (Human Combinatorial Antibody Library) is a unique library of recombinant Fab antibodies that encompasses the diversity of human antibodies and is developed as a high-quality library to produce highly specific artificial antibodies against various types of antigens. The HuCAL technology is based on the HuCAL PLATINUM library and CysDisplay technology developed by MorphoSys, and it is utilized by many customers worldwide as a very powerful tool for the production of fully human antibodies for research, diagnostics, and therapeutic purposes. *For more details, please refer to the PDF document or feel free to contact us.*

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Monoclonal Antibody Production Contract Service: Technical Information 3

Monoclonal Antibody Production Contract Service: Technical Information 3

Unique Antibody Production Technology - HuCAL Technology

The Antibody Craftsman's monoclonal antibody production service utilizes unique technologies such as phage display, strategic antibody selection, and high-throughput processes to select specific Fab antibodies against various antigens. This process enables us to provide antibodies with specialized specificities tailored to our customers' needs and to prepare antibody formats in a short period. HuCAL PLATINUM Library The HuCAL PLATINUM, consisting of approximately 45 billion patterns, is the world's largest high-quality antibody library. This library is based on a combination of genes for complementarity-determining regions (CDRs) with high diversity and framework master genes, optimized for expression in E. coli. *For more details, please refer to the PDF materials or feel free to contact us.

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Monoclonal Antibody Production Contract Service Prices

Monoclonal Antibody Production Contract Service Prices

List of Optional Services

In addition to the menu below, we can provide proposals tailored to your requests, so please feel free to consult with us. List of Optional Services ■ Affinity enhancement after antibody production ■ Production of various tags and fusion proteins ■ Format conversion ■ Production of sandwich pairs Please inquire for confirmation regarding the prices listed above. *For more details, please refer to the PDF document or feel free to contact us.

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Monoclonal Antibody Production Contract Service Q&A

Monoclonal Antibody Production Contract Service Q&A

Frequently Asked Questions Part 3: How long does it take to produce antibodies?

Question: How long does it take to produce antibodies? Answer: It takes at least 7 weeks from the start of antigen screening. It is possible to produce them in a shorter period compared to the monoclonal antibody production method using mice. *For more detailed information about the service, please refer to the PDF document or feel free to contact us.*

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