同仁グローカル List of Products and Services

Our company conducts antioxidant capacity analysis using DPPH. By using Trolox, an antioxidant substance, the results are expressed as Trolox Equivalent Antioxidant Capacity (TEAC) values. Additionally, it is used as a simple method to evaluate the radical scavenging activity for measuring the antioxidant capacity of various food ingredients and processed products. [Analysis Examples] ■ Functional foods, cosmetic ingredients ■ Lipid-soluble extracts ■ Processed products *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

Our company conducts activity analysis using the WST method, which can detect SOD activity (superoxide dismutase activity) with high sensitivity. We have a track record of analyzing SOD-like activity in functional foods, cosmetic ingredients, and extracts from plants, among others. The antioxidant capacity of food is thought to be related to the microbial flora in the body, and it can be viewed as a simple method to evaluate the radical scavenging activity possessed by food. [Analysis Examples] - SOD-like activity in functional foods and cosmetic ingredients - SOD-like activity in extracts from plants, etc. - SOD-like activity in samples pre-treated by dialysis, etc. - SOD-like activity in processed products *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Substance Analysis: Resin-based Samples + Verification Testing." The testing will include visual observation and verification testing (estimation of contamination timing). We can create reports tailored to various scenarios, such as internal documents and reports for end customers. For verification testing (estimation of contamination timing), we request that you provide one unit of the relevant product to create verification samples for contamination estimation, while we will prepare disposable chopsticks, toothpicks, and rubber bands. [Types of Samples] ■ Toothpicks ■ Disposable chopsticks ■ Small branches, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Substance Analysis - Inorganic Samples." The testing involves visual observation plus SEM-EDX (component identification). The identification of components using EDX allows for the acquisition of mapping images of the measured elements, enabling the understanding of the shape and composition of the foreign substances. Additionally, we can create reports tailored to specific scenes, such as internal company documents. 【Types of Samples】 ■ Metal fragments ■ Minerals ■ Rust ■ Ash ■ Plated products, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce the "Inhibition Activity Analysis" conducted by Dojin Glocal Co., Ltd. To analyze the inhibition activity against an enzyme, the concentration of the inhibitor that inhibits 50% of a certain enzyme reaction under specific conditions is defined as one unit. For example, when evaluating an inhibitor against amylase, inhibitors of varying concentrations are added, and the concentration of the inhibitor that reduces the amount of reducing sugars produced by amylase by half is determined. [Examples of Enzyme Activity Analysis] ■ Various enzyme activity analyses of extracts and raw materials ■ Enzyme activity analysis during processing of food materials (powders, solutions, solids, etc.) ■ Activity analysis of functional components (enzyme activity, inhibition activity, antioxidant capacity, etc.) ■ International unit representation of enzyme activity such as lipase *For more details, please refer to the PDF document or feel free to contact us.

• Other Analysis

We would like to introduce our "Food Foreign Substance Analysis: Product Discoloration and Spoilage." As for the testing, we respond with visual observation (cause estimation) plus confirmation tests (catalase test, metal coloration test, etc.). We assess the situation and propose appropriate tests. Additionally, we can create reports tailored to various scenarios, such as internal documents and reports for end customers. 【Types of Samples】 ■ The product is discolored ■ The product is colored ■ The product is spoiled *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Substance Analysis - Biological Samples." The tests include visual observation (species identification) and confirmation tests (such as the catalase test). The catalase test estimates the contamination period based on the presence or absence of the catalase reaction. Additionally, we carefully listen to the occurrence (manifestation) situation and will propose appropriate tests based on the circumstances. 【Types of Samples (Partial)】 ■ Insects ■ Hair ■ Plant fragments ■ Bones ■ Teeth *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Substance Analysis: Resin-based Samples + Verification Testing." The testing includes visual observation, IR (component identification), and verification testing (estimation of contamination timing). We will assess the situation and propose appropriate tests. For estimating the contamination timing, we require one unit of the relevant product to create a verification sample for contamination estimation. We will prepare chopsticks, toothpicks, and rubber bands. 【Types of Samples】 ■ Rubber bands ■ Pieces of plastic ■ Pieces of resin, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

Our company conducts food foreign matter analysis of resin-based samples. The testing includes visual observation and FTIR (component identification and source estimation). We also assess the situation and propose appropriate tests. Analysis using FT-IR can reflect the differences between regular rust and rust caused by iron bacteria. 【Types of Samples】 ■ Rubber pieces ■ Fibers ■ Threads, etc. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Matter Analysis: Paper and Cardboard Fragments." As part of the testing, we conduct visual observations and estimate the circumstances of occurrence based on the rules and conditions at the time of opening the raw materials, as well as the shape of the foreign matter. Additionally, we can create reports tailored to different scenarios, such as internal documents and reports for end customers. [Key Points] ■ We will carefully listen to the circumstances of occurrence. ■ We will understand the situation and propose appropriate tests. ■ We can create reports tailored to different scenarios. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

Our company conducts data analysis as part of food foreign matter analysis. Based on the microscope images, results of simple tests, FTIR charts, and EDX analysis results you provided, we will prepare a report and inform you. For those struggling with the analysis of acquired images and IR data, we will summarize our insights based on our extensive experience and achievements. 【Test Overview】 ■ Photos ■ IR Data ■ SEM-EDX Data *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce our "Food Foreign Substance Analysis - Trust Us Course." In this course, we carefully listen to the occurrence (manifestation) situation and propose appropriate tests based on our understanding of the circumstances. Additionally, we can create reports tailored to various scenarios, such as internal documents and reports for end customers. If you have any requests regarding the investigation, please let us know. 【Test Overview】 ■ Visual Observation (Estimation) ■ Proposal for Additional Investigation (+α) ・IR or SEM-EDX *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce the removal of biofilms using sodium hypochlorite solution based on the biofilm-related analysis conducted by our company. The effectiveness is quicker at higher concentrations, and at a 5% concentration, biofilms can be removed in just a few minutes. The effectiveness of biofilm removal also varies depending on the type of bacteria and the amount of biofilm present. It is also important to properly control the amount of biofilm formation to evaluate the effectiveness of the cleaning agent. 【Features】 ■ The higher the concentration, the quicker the removal; at a 5% concentration, removal is possible in just a few minutes. ■ The amount of biofilm decreases in a concentration-dependent manner with sodium hypochlorite. *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce the differences in biofilm formation by bacterial species based on the biofilm-related analyses conducted by our company. Many bacteria, from well-known ones like Escherichia coli and Pseudomonas aeruginosa to anaerobic bacteria such as periodontal pathogens, form biofilms. While the formation is influenced by culture conditions, the amount and structure of biofilm formation vary by species. Additionally, the biofilm formation by mixed bacteria can significantly exceed that of single species, and their morphology can differ greatly. [Differences by Bacterial Species] ■ Bacterial species with high biofilm formation: Pseudomonas aeruginosa, Staphylococcus epidermidis, Streptococcus mutans ■ Bacterial species with low biofilm formation: Escherichia coli, Staphylococcus aureus ■ Bacterial species that form highly adhesive biofilms: Staphylococcus epidermidis ■ Bacterial species that form less adhesive biofilms: Pseudomonas aeruginosa *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce what can be understood through biofilm analysis. It is utilized for evaluating the biofilm formation inhibition effects of panels and components used in various household appliances such as refrigerators, washing machines, and air conditioners, as well as for evaluating the biofilm formation inhibition effects of indwelling catheters. Our company has numerous achievements in evaluating biofilm formation suppression and removal capabilities using various materials and agents, so we can propose analysis systems tailored to your objectives and requests. 【Evaluation Achievements (Partial)】 ■ Evaluation of biofilm formation inhibition effects on materials used in bathtubs and piping ■ Evaluation of biofilm removal effects of cleaning agents ■ Evaluation of the inhibition effects on bacterial growth within biofilms by disinfectants, as well as evaluation of their sterilization effects ■ Evaluation of biofilm formation inhibition effects by surface treatment agents *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

We would like to introduce the "Measurement Method for Biofilms" conducted by our company. Typically, to detect biofilms, a dye called crystal violet is used. This cationic blue dye compound strongly binds electrostatically to the anions of the biofilm constituents in aqueous solution. In organic solvents, the binding weakens, allowing the crystal violet that was bound to the biofilm to be extracted by the organic solvent. 【Other Methods】 ■ Sensory Testing ■ Gravimetric Method *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and evaluation services

"Biofilm" is formed in environments where bacteria are present, provided there are adequate nutrients and moisture, and can develop not only at room temperature but also at low temperatures, characterized by its sliminess. Much of the sliminess is due to polysaccharides released by the bacteria. The majority of the volume consists of high molecular weight sugars (extracellular polysaccharides: EPS) that are produced by microorganisms. The type of biofilm that forms varies depending on the environmental conditions and the degree of bacterial species mixing and growth. [Examples of occurrence locations (partial)] ■ Bathroom and bathtub ■ Washing machine drum ■ Kitchen ■ Refrigerator ■ Air conditioner *For more details, please refer to the PDF document or feel free to contact us.

• Bio-related product films and sheets

We would like to introduce our "Evaluation Method for the Destruction and Removal Effects of Biofilms," which is part of the biofilm-related analysis we conduct. When evaluating the function of cleaning agents that destroy and remove formed biofilms, we generally use a 96-well plate to allow biofilms to form on each well. The biofilms are then immersed in the cleaning agent for a specified period, and the amount of biofilm removal is compared against sterilized water (or sterilized buffer, etc.) as a control. 【Analysis Method】 1. Immerse the substrate or pins in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pins. 2. After immersing the substrate or pins in the biofilm cleaning agent for a specified time, rinse with water. 3. Stain the substrate or pins with crystal violet. 4. Extract the crystal violet and measure the absorbance at 590 nm. 5. Compare the biofilm amounts between the control and the samples. *For more details, please refer to the PDF document or feel free to contact us.

• Other Testing Contract

We would like to introduce our analysis related to biofilms, specifically the "Evaluation Method for Bacterial Sterilization and Growth Inhibition in Biofilms." The sterilization effect is evaluated by measuring the metabolic activity of the bacteria. WST (water-soluble tetrazolium salt) is one of the reagents used to determine bacterial metabolic activity, producing WST formazan in accordance with the metabolic activity of the bacteria. By measuring the absorbance, we can determine the amount of surviving bacteria. 【Analysis Method】 1. Immerse the substrate or pin in the bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pin. 2. After immersing the substrate or pin in the sterilizing agent solution for a specified time, wash it with water. 3. Immerse the substrate or pin in the WST reagent for 10 to 60 minutes. 4. Measure the absorbance at 460 nm. 5. Compare the standard solution with the amount of biofilm. *For more details, please refer to the PDF document or feel free to contact us.

• Other Testing Contract

We would like to introduce our "Biofilm Formation Suppression Evaluation Method on Substrates," which is part of our biofilm-related analysis. To evaluate the suppression of biofilm formation, the substrate is first immersed in a bacterial culture solution to allow biofilm formation. The formed biofilm is then stained with crystal violet (CV), after which the CV is extracted with an extraction solution, and the absorbance is measured. This is compared to the biofilm formation amount on a standard substrate. 【Analysis Method】 1. Immerse the substrate in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours. 2. Rinse the substrate with water and stain the formed biofilm with crystal violet. 3. Extract the crystal violet and measure the absorbance at 590 nm. 4. Compare with the biofilm formation amount on the standard substrate. *For more details, please refer to the PDF document or feel free to contact us.

• Other Testing Contract

Our analysis experts not only identify foreign substances but also include analyses of the origin and timing of contamination, providing proposals for problem-solving. Regarding samples, depending on the test content, a length of about 1mm is sufficient. Even smaller samples may be tested in some cases. 【The Necessity of Foreign Substance Investigation】 ■ Complaints arising from foreign substance contamination or defective products directly affect customer trust. ■ By identifying the locations of contamination and the causes of defects, improvements can be made in the process, leading to enhanced quality. ■ Identifying the substances of foreign materials and defective products is crucial for problem-solving and process improvement. *For more details, please refer to the related links or feel free to contact us.

• Other analysis and evaluation services

We offer antioxidant activity measurement using SOD-like activity analysis with WST-1 and antioxidant capacity analysis with DPPH. We can propose various methods for measuring the antioxidant capacity of samples, whether you want to know if a sample has antioxidant activity or quantify the antioxidant activity of a sample (units/weight, volume). This can be seen as a simple method to evaluate the radical scavenging activity of food. 【Measurement Methods】 ■ SOD-like activity measurement method using WST-1 ■ Radical scavenging activity measurement method using DPPH *For more details, please refer to the related links or feel free to contact us.

• Other analysis and evaluation services

We conduct contract analysis of enzyme activity for various samples. We propose activity analysis methods tailored to your needs, such as "I want to measure the enzyme activity of a sample" or "I want to measure the inhibitory activity against a specific enzyme." Additionally, please feel free to inquire about enzyme inhibition activity analysis or any analyses not listed. We also conduct studies on the enzyme treatment conditions of samples. 【Analysis Examples】 ■ Analysis of enzyme activity in various extracts and raw materials ■ Analysis of enzyme activity during processing of food raw materials (powders, solutions, solids, etc.) ■ Activity analysis of functional components (enzyme activity, inhibitory activity, antioxidant capacity, etc.) ■ International unit representation of enzyme activity such as lipase *For more details, please refer to the related links or feel free to contact us.

• Ingredient analysis

We would like to introduce our "Anti-Biofilm Test." We propose biofilm-related evaluation tests tailored to purposes such as "wanting to assess the decomposition effect of cleaning solutions on biofilms" or "wanting to determine the minimum concentration that does not form biofilms in samples." Additionally, the microbial species used in the analysis include Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus mutans, but please feel free to inquire about analyses with other microbial species or analyses using collected microbes. 【Analysis Examples】 ■ Evaluation of the effect of inhibiting biofilm formation by bacteria ■ Evaluation of the destruction (removal, decomposition) effect of biofilms formed by bacteria ■ Evaluation of bactericidal and growth inhibition effects on bacteria within biofilms formed by bacteria ■ Evaluation of biofilm formation inhibition on treated surfaces, substrates, etc. ■ Evaluation of biofilm formation suppression around treated surfaces, substrates, etc. *For more details, please refer to the related links or feel free to contact us.

• Other analysis and evaluation services

Our company conducts "fatty acid analysis" on various samples such as animal tissues, plant tissues, blood, cells, bacteria, culture media, feed, fresh food, grains, and processed or fermented foods. We propose analyses tailored to your needs, including sample pretreatment methods. We can also accommodate tests using small amounts of precious samples like cells, tissues, and blood. If you are unsure about "sample pretreatment methods or how to conduct fatty acid analysis" or if you wish to perform fatty acid analysis for research, product development, or quality control, please feel free to consult us. 【Fatty Acid Analysis】 ◇ Fatty Acid Composition Analysis ◇ Quantitative Analysis of Fatty Acids 【Analysis Examples】 ■ Fatty acids contained in animal tissues (cells) ■ Fatty acids contained in plant tissues ■ Fatty acids from microorganisms ■ Fatty acids on the surface of insects ■ Differences in fatty acid composition by fish parts ■ Differences in fatty acid composition by tea leaf types ■ Analysis by lipid fractionation ■ Fatty acid analysis by type of lipid *For more details, please refer to the related links or feel free to contact us.

• Other analysis and evaluation services