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Support for the daily HPLC routine has been strengthened, ensuring the reliability of results. By making wise investments, you can enhance the sustainability of the lab.
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Free membership registrationPFAS, the collective term for perfluoroalkyl substances (PFOA) and polyfluoroalkyl substances (PFOS), is increasingly facing global regulatory guidance and stringent requirements due to research findings indicating health concerns related to their persistence and accumulation in the body. Agilent supports PFAS analysis using LC/MS based on years of accumulated expertise both domestically and internationally.
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Free membership registrationThe Agilent Advanced Dilution System 2 (ADS 2) is an integrated automated dilution system. By automating cumbersome procedures that are prone to errors, such as the preparation of standard solutions, dilution of samples prior to analysis, and pre-treatment for re-dilution of samples or QC solutions that fail outside the calibration range, it simplifies lab workflows. Automating these tasks saves analysts' time, reduces the risk of human error, and improves sample throughput. ADS 2 seamlessly integrates with Agilent ICP-OES and ICP-MS. It is incorporated into ICP Expert and ICP-MS MassHunter software, allowing for complete control. This document explains the changes that ADS 2, designed with productivity and simplicity in mind, brings to lab workflows.
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Free membership registrationThe new Agilent Infinity III LC is a liquid chromatograph that automates and remote-controls analytical workflows, making your lab smarter. It streamlines routine and ancillary tasks, allowing you to focus on R&D and creative work, while reducing human error and stress. **Features** - **Automation and Remote Control** Streamlines daily tasks from mobile phase handling to sample management. You can monitor the status of the device from your office or home. - **Maximizing Uptime** Uses troubleshooting and maintenance guides to maximize device uptime. Even if the LC personnel change due to organizational restructuring or employee turnover, the system can be fully operational immediately. - **Investment Protection** Existing Infinity II LC systems can be easily upgraded with the latest Agilent Infinity III LC technology. *Please download the leaflet with a comparison table to conventional HPLC here (PDF).*
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Free membership registrationI have summarized the essential basic terms necessary for understanding chromatography. Additionally, I will introduce examples of outputs for each chromatographic parameter as described in the basic chromatography terms using the Agilent data system. This document clearly presents parameters of chromatograms, such as hold-up time (the elution time of non-retained components) and peak height. Please feel free to download it and read through it. 【Contents (partial)】 ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection ■ Peak Identification and Quantification in HPLC *For more details, please download the PDF or feel free to contact us.
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Free membership registrationPeak identification in HPLC is performed based on the retention time of peaks from standard samples. When information such as the UV-Vis spectrum from a DAD is simultaneously obtained, identification can also be done by considering the spectral matching. Quantification of the analyte is carried out by preparing a concentration series of standard sample solutions, determining the peak area or peak height from the chromatogram, and creating a calibration curve. This document provides an easy-to-understand explanation of peak identification and quantification in HPLC using diagrams, and also introduces absolute calibration curves and internal standard methods for quantification. We encourage you to read it. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection ■ Peak Identification and Quantification in HPLC *For more details, please download the PDF or feel free to contact us.
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Free membership registrationThe evaporative light scattering detector (ELSD) detects the scattered light from particles of the analyte after evaporating and removing the mobile phase from the eluent coming from the column. Since the mobile phase must be volatile, mobile phases containing non-volatile salts such as phosphates cannot be used. Additionally, analytes that are highly volatile cannot be applied as they will evaporate along with the mobile phase. However, it is possible to detect compounds that do not have UV-Vis absorption or fluorescence. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationThe differential refractive index detector (RID) detects the difference in refractive index of the eluent from the column containing the mobile phase and analytes. It can be used for the detection of all compounds, but its sensitivity is not very good, estimated to be less than 1/1000 of that of a UV-Vis detector. It can detect compounds that do not have UV-visible absorption or fluorescence, making it useful for detecting sugars and synthetic polymers. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationWhen light is applied, electrons are excited, and the phenomenon of light emission when the excited electrons return to the ground state is called fluorescence. Substances that emit fluorescence are referred to as fluorescent materials, and such compounds can be detected using fluorescence detectors (FLD). The wavelengths of the excitation light and fluorescence are specific to the fluorescent material, making FLDs superior in sensitivity and selectivity compared to UV-Vis detectors. This document provides a clear summary of the basics of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationDAD, unlike VWD, irradiates the cell with light from the light source without spectrally analyzing it. The light that passes through the cell is spectrally analyzed and detected by a device with an array of photodiodes (photodiode array). It is possible to record the UV-Vis spectrum of the eluent from the column. Therefore, simultaneous multi-wavelength detection, peak purity testing, and comparison with the UV-Vis spectrum of standard substances can be performed, making the peak identification capability superior to that of VWD. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationVWD spectrally analyzes the light from the light source using a grating, converts it into monochromatic light, and then measures the absorbance in a flow cell. Since detection is performed at a single wavelength, it is possible to switch wavelengths using a time program for simultaneous analysis of multiple components when the maximum absorption of each analyte differs. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationMany organic compounds analyzed by HPLC have absorption in the ultraviolet-visible region. Therefore, ultraviolet-visible absorbance detectors are the most widely used in HPLC. A deuterium discharge lamp (D2 lamp) is used as the light source, but a tungsten lamp may also be used in conjunction to measure absorption in the visible region with high sensitivity. This document provides a clear summary of the basics of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationChromatography is a separation technique, and by appropriately combining it with detectors that can detect the separated analytes, quantitative analysis becomes possible. Detectors need to be selected considering applicable compounds, sensitivity, selectivity, and other factors. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationIn HPLC, there are isocratic elution, which maintains the composition of the mobile phase for separation, and gradient elution, which varies the composition of the mobile phase for separation. Generally, isocratic elution is used for analyzing multiple components where there is little difference in retention time for each component, while gradient elution is used for analyzing multiple components with significant differences in retention time. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationAffinity chromatography is a type of chromatography that utilizes the high affinity of biomolecules such as proteins, and is used for extracting and purifying specific proteins from cell culture supernatants. By downloading the materials, you can view a diagram showing the purification of IgG from CHO cell culture supernatant using an affinity column with immobilized Protein A. This document provides a clear summary of the fundamentals of liquid chromatography (LC), including separation basics, detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationSize exclusion chromatography separates compounds based on differences in molecular size of the analytes in the mobile phase, rather than interactions between the stationary phase and the analytes. Molecules smaller than the pore size of the packing material pass slowly through the column, while molecules larger than the pore size pass quickly through the column. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is a valuable resource for those learning HPLC for the first time, those who want to review the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationIon exclusion chromatography uses ion exchange resin as the stationary phase, but unlike ion exchange chromatography, it utilizes the electrostatic repulsive forces between the ion exchange groups and the analytes. In the case of weak acids such as organic acids, the degree of dissociation in acidic solutions varies depending on the compound, leading to differences in charge strength. For analytes that have the same sign of charge as the ion exchange groups, the repulsive force received from the ion exchange groups varies according to the strength of that charge. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is a valuable resource for those learning HPLC for the first time, those who wish to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationIon exchange chromatography separates ionic compounds using a stationary phase that incorporates ion exchange groups (such as sulfonic groups for cation exchange or quaternary ammonium groups for anion exchange) and a mobile phase that consists of an aqueous solution containing salts, such as a buffer. It is used for the separation of amino acids, organic acids, and proteins. By downloading the materials, you can view examples of protein analysis using cation exchange columns. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who wish to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationHydrophilic interaction chromatography (HILIC) uses a stationary phase consisting of high-polarity bonded phases such as amino groups, with packing materials or silica gel, and a mobile phase of a mixed solution of water (buffer) and acetonitrile. It is suitable for the separation of compounds with weak retention (high polarity) in RPC. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationIon-pair chromatography involves adding ionic compounds known as ion-pair reagents (such as alkyl sulfonates and quaternary ammonium salts) to the mobile phase to separate ionic compounds that are not retained in RPC using the stationary phase of RPC. Ion-pair reagents are used that have charges opposite to those of the analytes; for anionic compounds, quaternary ammonium salts are used, and for cationic compounds, alkyl sulfonates are used. This document provides a clear summary of the basics of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationReverse phase liquid chromatography uses a packing material with a low-polarity chemically bonded phase as the stationary phase and a high-polarity solvent as the mobile phase. High-polarity compounds are less retained by the stationary phase and elute quickly, while low-polarity compounds elute more slowly. Octadecylsilyl silica gel (ODS), which incorporates octadecyl groups (C18) into silica gel, is a widely used packing material in HPLC. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to HPLC, want to review from the basics, or are looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationNormal-phase liquid chromatography uses a stationary phase consisting of a highly polar chemically bonded phase and a mobile phase of low-polarity solvents. Because highly polar compounds are strongly retained by the stationary phase, low-polarity compounds elute quickly. It is used for the analysis of lipophilic compounds and the separation of organic compounds by groups. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationPartition chromatography separates compounds by utilizing the differences in the distribution equilibrium of the analyte with respect to the stationary phase. The stationary phase typically consists of a chemically bonded packing material known as a bonded phase. There are two types of partition chromatography: normal phase and reverse phase. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is a valuable resource for those learning HPLC for the first time, those looking to review from the basics, and those seeking materials for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationAdsorption chromatography separates compounds by utilizing the difference in adsorption equilibrium between the adsorption sites of the stationary phase (packing material) and the analytes. Silica gel and alumina are used as the stationary phase, while hexane and dichloromethane are used as the mobile phase. It is effective for the separation of isomers. This document provides a clear summary of the basics of liquid chromatography (LC), detectors, and principles. It is a valuable resource for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationIn chromatography, the differences in the affinity of substances for the stationary phase and the mobile phase are utilized. The separation modes commonly used in HPLC can be broadly classified into adsorption, partition, and size exclusion based on the type of affinity. The main separation modes include "adsorption," "partition," "hydrophilic interaction," "ion exchange," and "size exclusion." This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are learning HPLC for the first time, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationHigh performance liquid chromatography (HPLC) is a type of liquid chromatography that achieves high separation efficiency by pressurizing the mobile phase liquid and using high-performance columns. It is utilized in various fields of chemical analysis. The HPLC system consists of a pump that delivers the mobile phase, an injector for sample injection, a column that separates the components in the sample, a column thermostat that maintains a constant column temperature, and a detector that identifies the separated components. This document provides a clear summary of the fundamentals of liquid chromatography (LC) separation, detectors, and principles. It is a valuable resource for those who are learning HPLC for the first time, those who wish to review from the basics, and those looking for educational materials for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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Free membership registrationThe Bioz implementation is currently on the web pages of the three Bio LC products. <What is Bioz?> It is a service that uses an AI-powered widget (a small card) to present product citations and images, providing customers with compelling scientific papers. ★95/100 Bioz Rating | 41,150 citations After accessing the homepage, wait a few seconds, and the English scientific paper information will appear at the top of the page.
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Free membership registrationA single-channel gas chromatograph that achieves a smarter and more efficient sample flow while maintaining lab space. The Agilent 8850 GC is the smallest high-performance benchtop GC on the market, occupying only half the installation space of traditional equipment. It combines powerful intelligence features, reliable performance, unparalleled reproducibility, accuracy, sensitivity, and compact installation area. Additionally, it consumes up to 30% less power compared to standard GCs, helping to achieve sustainability goals.
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Free membership registrationThe listed price is an estimated price (including tax) for academia (non-profit organizations such as educational and research institutions). Customers outside of academia are kindly requested to inquire separately.
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Free membership registrationBy increasing the surface area, we enhance the opportunities for adsorption and distribution between the analyte and functional groups, thereby improving efficiency. SPEC demonstrates very stable reproducibility and high flow characteristics even when processing high-viscosity or multi-particle samples. As a result, it enables high reproducibility of recovery rates and rapid operations, allowing for trouble-free automation. Additionally, since each of the 96 wells flows at a uniform flow rate without fluctuations, the variability in recovery rates for each well can be kept very low. Most types of packed SPE require 500 to 1000 μL of elution solvent. When analyzing with LC/MS/MS after processing, further operations such as evaporation and re-dissolution are necessary to achieve the required sensitivity. In the case of SPEC, substances retained in the porous surface can be eluted with only about 75 μL of solvent. By eluting with a minimal amount of solvent, the target substances are highly concentrated, allowing for direct injection into LC/MS/MS, which contributes to saving time and solvent volume, and improving productivity. *For more details, please refer to the PDF document or feel free to contact us.*
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Free membership registrationThis trap does not convert oxygen into another contaminant; it actually removes oxygen. Oxygen and many oxides react with the activated filler to form manganese oxide. As a result of this reaction, the indicator gradually changes from a light green color, indicating the activity of the adsorbent, to a dark brown color, indicating oxygen saturation. This indicator oxygen trap is designed to be used in conjunction with a large-capacity oxygen trap that does not have an indicator. Therefore, this indicator-equipped oxygen trap is typically installed downstream of the trap without an indicator. By doing so, the contamination can be visibly confirmed in the indicator oxygen trap before it reaches the equipment. Agilent's indicator material does not undergo exothermic reactions when exposed to air or water. The used product is non-toxic and harmless, and it is neither flammable nor reactive. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationThe Agilent indicator-equipped moisture trap can remove water, oil, and organic compounds from gases used in gas chromatography (or other analyses). This moisture trap is refillable and is made using polycarbonate tubes manufactured by Lenax. It is lighter and more chemically resistant compared to acrylic traps, and significantly more durable. This indicator-equipped trap is currently used as the standard trap for removing moisture from GC gases in chromatographic analysis. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationAgilent's fittings and joints for equipment piping are available in brass, stainless steel, and UltiMetal Plus, with sizes ranging from 1/16 inch to 1/4 inch. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationAgilent's high-quality copper, stainless steel, and UltiMetal Plus tubes are manufactured and cleaned for chromatography. We offer tubes in multiple lengths, so you can choose from 1/16 inch, 1/8 inch, and 1/4 inch to meet your flow requirements. The UltiMetal Plus tube is 1/16 inch and is suitable for inert environments. *For more details, please refer to the related links or feel free to contact us.
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Free membership registrationIn a 100cc unit, up to 30 ppm of moisture, or 16.3 grams of water, can be reduced to below 10 ppb using a standard set of 10 cylinders of 7m³. Molecular sieves possess unique characteristics among commercially available desiccants, strongly adsorbing water and polar compounds even at relatively low densities. In the center of the trap, there is a color-changing indicator that notifies you when it's time for replacement. Molecular sieves are not only of high quality but also the material of the trap head has been treated for high vacuum and high-temperature environments. Therefore, the efficiency and capacity of gas purification are at the highest level. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationThe split vent trap captures contaminants that come from the split injection system. Be sure to replace the split vent cartridge every six months. The internal split vent trap is installed at the factory to prevent clogging of the split vent line, which can cause system errors and costly repairs. Replacement cartridges are also available. The optional external trap enhances protective functions by filtering the split vent flow, preventing contamination of the lab by contaminants. *For more details, please refer to the related links or feel free to contact us.
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Free membership registrationAgilent offers disposable syringes in three sizes: 20mL, 10mL, and 5mL. Additionally, new 2mL and 1mL options have been added to the lineup. We provide 100 individually packaged sterilized syringes, as well as cost-effective bulk packs that are not sterilized. *For more details, please refer to the related links or feel free to contact us.
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Free membership registrationAgilent's precision gas flow meters are ideal for chromatography analysis, offering high accuracy and reliability according to NIST standards. They also provide industry-leading stability, eliminating the need for frequent recalibration over long periods. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationAgilent provides the tools and equipment necessary for the installation and maintenance of laboratory instruments. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationThe combination of oxygen/moisture adsorbents achieves two functions in one trap. This trap is non-refillable, but it excels in safety, performance, and cost compared to heated catalytic traps. The Agilent OT3 oxygen/moisture trap features an inert aluminum body containing highly active adsorbents that include metals, which remove 500 mL of oxygen from the gas, reducing the concentration to below 15 ppb. Additionally, it can remove up to 2 g of moisture without compromising the oxygen removal capacity. The oxygen/moisture trap is recommended for ECD (electron capture detector) and GC/MS applications. *For more details, please refer to the related links or feel free to contact us.
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Free membership registrationAgilent's manifolds and accessories further enhance the performance of solid-phase extraction adsorbents. They offer the convenience of being used both as a set and individually, allowing for the functionalities required at various stages, from method development to high-throughput analysis. A wide variety of adapters are available, enabling the stacking of cartridges in multiple steps and the use of two types of manifolds: 12-port and 20-port. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationThe large universal trap uses multiple adsorbents, all of which have been developed through rigorous testing and evaluation. One large universal trap has the capacity to purify the equivalent of 13 K-size cylinders of helium with a purity of 99.997%. The large universal trap offers superior economic efficiency compared to competitors' purifiers. Its capacity is nearly three times that of competing products. It is particularly commonly used in GC/MS applications. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationAgilent's hydrocarbon/water trap offers flexibility. It can be refilled and can remove extremely low amounts of hydrocarbons and moisture. *For more details, please refer to the related links or feel free to contact us.
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Free membership registrationAgilent's hydrocarbon trap can remove hydrocarbons down to extremely low levels. *For more details, please refer to the related links or feel free to contact us.*
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Free membership registrationIt provides a hydrophobic retention mechanism without the need for conditioning. High throughput has been significantly improved for field sampling in environmental applications. NEXUS offers reproducible high recovery rates. This capability has been achieved through the development of a unique cross-linked spherical polymer filler that can adsorb both polar and non-polar samples. The large particle size of NEXUS is suitable for the extraction of samples with high viscosity, such as horse urine. *For more details, please refer to the PDF document or feel free to contact us.*
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