アジレント・テクノロジー株式会社 List of Products and Services

High throughput has significantly improved field sampling for environmental applications. NEXUS WCX was developed as a solid-phase extraction column for the pretreatment of strongly basic compounds with functional groups such as quaternary amines. Like NEXUS, it is also suitable for extracting samples with high viscosity, such as horse urine. *For more details, please refer to the PDF document or feel free to contact us.

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The SPS 24 can process up to 24 SPE cartridges simultaneously. Like the Vac Elut 20 vacuum manifold, the SPS 24 is made of permanent solvent-resistant materials and is designed for long-term use. The glass material on the sides allows for easy visibility of the entire sample collection process. *For more details, please refer to the PDF materials or feel free to contact us.*

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The transparent glass base of the manifold allows for easy monitoring of the collection process for all samples. Its compact, box-type design can be used in very narrow bench spaces. The vacuum control valve, vacuum gauge, and quick-release valve of the Vac Elut 20 are located away from the liquid contact area, conveniently mounted on the lid for easy access. The solvent-resistant polypropylene rack is available in various sizes to accommodate the collection tubes used for sample recovery. Medical-grade polypropylene needles used to reduce sample carryover are low-cost, disposable, and easily replaceable. PP extension tips can also be used as a substitute for standard needle valves, ensuring direct recovery into the collection tubes. The identification of samples and their corresponding recovery tubes is also secured by the interlock structure between the lid and the internal test tube rack. *For more details, please refer to the PDF materials or feel free to contact us.*

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The Luer stopcock is used in conjunction with a vacuum manifold to individually control the flow rate of each Bond Elut cartridge. These are made of solvent-resistant polypropylene and can be easily cleaned. *For more details, please refer to the PDF materials or feel free to contact us.

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The Bond Elut 96 Square Well Plate is specially designed to achieve excellent cleanup performance, flow reproducibility, and reliability, enabling trouble-free high-throughput operation. Transferring methods from cartridge-based approaches to an automated 96-well plate format has become easier than ever. The 2mL wells accommodate the large processing volumes of previous methods, allowing for quick and easy method transfer and revalidation. With the large 2mL wells, you can flexibly respond during the development of new methods, for example, when a large amount of washing is required or when a significant amount of filler is needed. Furthermore, the Bond Elut 96 Square Well is designed to ensure compatibility with existing robots and vacuum manifolds. With Agilent's large-capacity bulk packages for SPE cartridges and plates, you can reduce costs and simplify inventory management. *For more details, please refer to the PDF document or feel free to contact us.

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Florisil is a silica gel that contains magnesium oxide. Like silica, it has a very high polarity by nature, making it suitable for the separation of polar compounds from non-polar matrices. This filler has a large particle size, allowing for high-speed flow of large volume samples. Therefore, it becomes an effective alternative to silica when the viscosity of the sample matrix is extremely high. With Agilent's large-capacity bulk packages for SPE cartridges and plates, you can reduce costs and simplify inventory management. *For more details, please refer to the PDF materials or feel free to contact us.

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Bond Elut Focus features a unique amide-based polar packing technology that excels in retaining both polar and non-polar target compounds. The extraction method is simple and highly versatile, eliminating the need for complex pH adjustments through ion exchange or mixed-mode techniques. The unique bonding phase of this packing material possesses hydrogen bonding capabilities, dramatically enhancing the retention of basic compounds. As Focus improves the retention of polar target compounds, it enables the use of a powerful "Power Rinse" without loss of the analyte. Washing with 10-20% acetonitrile and methanol can effectively remove endogenous matrix interferences. Highly polar interferences with strong polar interactions, such as atenolol, can be washed out up to 100%. *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut Plexa PCX is an advanced version of Bond Elut Plexa that can be used with a simple and reproducible method. Plexa PCX is a new polymer-based cation exchange solid-phase extraction mini-column that combines the excellent characteristics of Bond Elut Plexa—high flow rates and clean sample acquisition—with strong cation exchange functionality. The new concept SPE packing material, Plexa PCX, removes neutral/acidic impurities from the matrix, selectively recovers basic analytes, and improves quantitative sensitivity. *For more details, please refer to the PDF document or feel free to contact us.*

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The cartridge filled with ACS grade granular anhydrous sodium sulfate allows for an easy drying process using sodium sulfate. We offer three types of cartridges (LRC, Bond Elut Jr, straight barrel). *For more details, please refer to the PDF document or feel free to contact us.*

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Empore extraction disks can handle high-flow solutions for large-volume sample preparation and are available with various binding phases. Empore disks come in two diameters: 47mm and 90mm. A larger diameter disk increases the surface area of the disk that serves as the sample pathway, resulting in an increased flow rate of the solvent passing through the disk. *For more details, please refer to the PDF document or feel free to contact us.*

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The Bond Elut OMIX pipette tips allow for the reliable purification and concentration of peptides and proteins at fmol and pmol levels before MALDI-TOF or LC/MS/MS analysis. The Bond Elut OMIX employs a unique monolithic filler technology, which provides consistent flow rates and strong interactions between the analytes and the surface, resulting in stable performance that surpasses other tips. Available in three shapes, the Bond Elut OMIX tips bind a large number of peptides and achieve a high sequence coverage rate. *For more details, please refer to the PDF materials or feel free to contact us.*

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The ceramic homogenizer improves the overall productivity of the lab and the reliability of the analytical results. *For more details, please refer to the PDF document or feel free to contact us.

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The reliable silica-based/polymer-based packing material used in the standard Bond Elut cartridges can be utilized in the Bond Elut 96-well plates for high-throughput applications. Current cartridge-based SPE results can be quickly re-evaluated in a 96-well format. Bond Elut 96 is manufactured under strict quality control standards to ensure no elution, reproducibility of extraction and purification, and reliability. Additionally, everything is manufactured and stocked in-house at Agilent, allowing for prompt shipping. *For more details, please refer to the PDF materials or feel free to contact us.

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Bond Elut C18 is a highly hydrophobic silica-based packing material within the product line. It has extremely high retention characteristics for nonpolar compounds and is a popular packing material. C18 is a silica-based packing material that retains most organic compounds from an aqueous matrix, but it may be avoided due to its lack of selectivity. However, when the structures of the target compounds vary significantly and have a wide range of polarities, there is an advantage in retaining them all at once. The polarity interactions between the target compounds and the packing material are not significant due to the strong effect of long-chain hydrocarbons. When analyzing low molecular weight compounds, C18 does not retain salts and allows them to pass through, making it useful for desalting the aqueous matrix prior to ion exchange. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut AccuCAT is a mixed-mode SPE consisting of strong cation exchange (SCX) and strong anion exchange (SAX) sorbents. AccuCAT is effective for the extraction of acidic, basic, and neutral compounds from urine and other biological samples. AccuCAT is particularly effective for the extraction of catecholamines from body fluids. *For more details, please refer to the PDF materials or feel free to contact us.

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Alumina, like silica, is an extremely polar filler. The surface of alumina tends to be stable at a higher pH state than non-functionalized silica. Due to its fine particle size and high extraction efficiency, a small amount of filler (50 mg) is generally used. Bond Elut AL-A possesses Lewis acid characteristics and particularly retains electron-rich compounds. *For more details, please refer to the PDF document or feel free to contact us.*

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Alumina, like silica, is an extremely polar filler. The surface of alumina tends to be stable at a higher pH state compared to non-functionalized silica. Due to its fine particle size and high extraction efficiency, a small amount of filler (50 mg) is generally used. Bond Elut AL-B has the properties of an electron donor and retains compounds with cations and hydrogen bonds. *For more details, please refer to the PDF document or feel free to contact us.*

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Alumina, like silica, is an extremely polar filler. The surface of alumina tends to be stable at a higher pH state than non-functionalized silica. Due to its fine particle size and high extraction efficiency, a small amount of filler (50 mg) is generally used. Bond Elut AL-N has an electrically neutral surface, allowing it to retain compounds such as aromatic and aliphatic amines. It can also be used to extract either non-polar or polar compounds from hydrophilic or hydrophobic samples, respectively. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut Atrazine is designed specifically for the extraction of atrazine from aqueous matrices, as well as its dealkylated metabolites, desethylatrazine and deisopropylatrazine. The 120μm particle size and suitable tube diameter allow for fast flow rates, making it ideal for processing large volumes of water samples. Additionally, the bed mass capacity of Bond Elut Atrazine is optimized to ensure the reliable recovery of atrazine and its polar metabolites, demonstrating sufficient capacity. By using an analytical method that combines Bond Elut Atrazine with Varian's HPLC diode array detector system, quantitative analysis of atrazine and its dealkylated metabolites in river water is possible even at extremely low concentrations (0.02 ppb). This concentration level is significantly lower than the EPA's standard (3 ppb) and the values set by European agencies (0.1 ppb and 0.5 ppb). The rapid and highly reproducible extraction and analysis methods are reliable and can minimize the consumption of organic solvents. *For more details, please refer to the PDF materials or feel free to contact us.*

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Bond Elut C1 is a single carbon functional group, resulting in low retention capacity for all alkyl group bonds in non-polar compounds. However, due to the end-capping, the silanol activity is suppressed. As a result, the retention and elution of polar and multifunctional compounds become easier. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut C18 EWP (Extra Wide Pore) is a standard particle size silica, but due to its 500 Å pore size, it allows for more efficient extraction of macromolecules from biological fluids and aqueous matrices. This pore size can retain larger molecules (＞15,000 MW) that are typically excluded by standard pore sizes, interacting with the bonded silica surface within the pores. C18 EWP enables the separation of mixtures of proteins, peptides, and nucleotides. Additionally, in many analytical techniques such as LC/MS/MS and capillary electrophoresis, when salts in the sample affect the analysis, it can be selected as a good sorbent for desalting from matrices containing the target macromolecules. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut C18 OH is a non-end-capped octadecyl bonded phase, with the silanol groups on the silica surface being activated. The low-load C18 adjusts the activity of the silanol groups under strict QC standards to maintain the best reproducibility for each batch. The activity of the silanol groups enhances the separation and extraction capabilities of metabolites and the retention of basic compounds compared to end-capped C18. Additionally, the 150 Å pore size is suitable for moderately sized molecules that may not be retained by standard 60 Å packing materials. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut C2 is a short-chain alkyl functional group that is a more polar packing material compared to C8, with ethyl groups exposed on the silica surface. When target compounds are strongly retained by C18 or C8 packing materials, C2 is often used as an alternative. The polarity of C2 is slightly lower than that of CN in polar interactions. The selection of C2 is common for the extraction of drugs from plasma or serum. This is because, when proper washing and elution are performed, analytical interferences can be removed, resulting in a flat baseline. *For more details, please refer to the PDF materials or feel free to contact us.*

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Bond Elut C8 has very similar properties to C18, but as a short-chain hydrocarbon, it has weaker interactions with non-polar compounds compared to C18. When the target compound is strongly retained by C18, C8 can effectively elute and serve as an alternative to C18. Since short-chain hydrocarbons cannot effectively mask the silica surface, the interaction strength between C8 and polar compounds is somewhat higher than that of C18. However, the interaction with polar compounds is not a prominent characteristic of C8. C8 packing materials are widely used for the simultaneous extraction of fat-soluble/water-soluble vitamins from human serum. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut Cellulose is filled in a clean 3mL polypropylene cartridge with minimal elution. The granulated cellulose powder bed is secured in place with two 20μm polyethylene frits. The unique rod shape and precisely adjusted cellulose particles maintain a free-flowing feel even when dry. For the cellulose raw material, high-purity cotton with a very high cellulose content is used to ensure optimal packing characteristics of the column. This results in the production of a high-density, highly stable, smoothly packed head column. In addition, the cellulose phase is very stable over a wide range of pH values and has an extremely low metal content (Fe and Cu content is below 5ppm). The combination of surface area and polymer structure results in a packing material with excellent retention capacity. *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut Certify is a mixed-mode solid-phase extraction (SPE) sorbent designed for the extraction of basic (cationic) drugs from biological matrices such as urine, plasma, and serum. Target compounds are strongly retained by Certify, which has both ion exchange and non-polar secondary interactions. By using forced buffers or organic solvent/water solutions, non-polar, polar, and ionic matrix contaminants can be washed away. Its excellent sample cleanup and concentration capabilities provide clean and clear samples for screening by GC, GC/MS, LC/MS, or TLC. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut Certify II is a specially designed cartridge for the rapid and effective extraction of acidic drugs and metabolites from biological matrices such as urine. It has been optimized for the extraction of acidic drugs, such as salicylic acid, ibuprofen, and acetaminophen, which possess non-polar and anionic properties. *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut CH is a medium-polarity sorbent that exhibits unique selectivity for certain compounds. In comparison to reversed-phase sorbents, CH has a polarity similar to C2. The ability to retain polar substances, such as phenolic compounds, from an aqueous matrix is an important factor among the characteristics of CH. Due to its selectivity, when target compounds cannot be retained by other non-polar sorbents like C18, C8, C2, and PH, CH serves as a good alternative. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut CN-E is a medium reverse phase stationary phase with many application examples. It is ideal for applications involving extremely nonpolar compounds that are irreversibly retained by reverse phase stationary phases such as C18 and C8. End-capped cyano stationary phases are very useful when extracting compounds from aqueous matrices. *For more details, please refer to the PDF materials or feel free to contact us.*

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The characteristics of Bond Elut DEA and Bond Elut NH2 are similar in several respects, and they are also somewhat akin to Bond Elut PSA. DEA has a slightly lower exchange capacity (1.0 meq/g) as an anion exchange packing material compared to NH2, and the addition of a carbon chain to the functional group has further increased its hydrophobicity. Even with the presence of the main functional group, DEA also functions as a packing material with moderate polarity due to the carbon chain. While DEA is somewhat more polar than C8, it is less polar than C2 or CN. *For more details, please refer to the PDF document or feel free to contact us.*

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Diol (2OH) is typically used to extract polar substances from non-polar solvents. The strong hydrogen bonding capability of diol is similar to that of non-bonded silica, and its ability to distinguish between similar compounds, such as structural isomers, is common with the main capabilities of silica. The separation of prostaglandins using diol can be achieved by gradually changing the polarity of the solvent during elution. Diol is a filler with higher reproducibility compared to silica in normal phase systems, allowing for predictable results. Diol can also be used to retain non-polar compounds due to hydrocarbon spacers. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut ENV, a high-purity styrene-divinylbenzene (SDVB) polymer, was developed for the extraction of herbicide metabolites and polar organic residues such as explosives from large volumes of water samples. The feature of Bond Elut ENV is its 125μm high cross-linking spherical polymer, which can process 1L of water sample in a very short time compared to the time required for other sample pretreatments. It elutes a wide range of compounds in a single elution step, even without secondary interactions on the polymer surface. Primarily used for environmental applications, Bond Elut ENV can also be utilized for clinical applications, including the extraction of highly polar metabolites such as glucuronide-conjugated compounds from human body fluids. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut LMS elutes target compounds without the addition of amines, buffers, or acids. The polymer, which eliminates secondary interactions, means that elution of compounds can be achieved with organic solvents or low ionic strength HPLC mobile phase solvents. This feature ensures compatibility with precision analytical techniques such as LC/MS. Bond Elut LMS is characterized by an optimized particle size and a large surface area that enhances extraction efficiency, without interfering with clear extraction. Bond Elut LMS ensures excellent recovery rates and reproducibility, reducing downtime and ensuring trouble-free operation. *For more details, please refer to the PDF materials or feel free to contact us.*

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Bond Elut Mycotoxin is a solid-phase extraction mini-column that effectively extracts trichothecenes and zearalenone from food. Compared to immunoaffinity columns (IAC) and charcoal/alumina columns, it provides equivalent or better results. Recovery rates and other factors were examined in foods such as wheat, corn, macaroni wheat, barley, bread, and muesli. In Bond Elut Mycotoxin, the target substances pass through while other matrix components are retained. *For more details, please refer to the PDF document or feel free to contact us.*

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We would like to introduce the 'Bond Elut NH2' that we handle. It is a sample preparation product, available in two options: "500mg, 100 pieces" and "1000mg, 100 pieces." Please feel free to contact us if you would like to place an order. *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut PBA is a unique packing material that binds phenylboronic acid to a silica gel surface. The strength of the covalent bond is due to interactions that are 10 to 100 times greater than other extraction mechanisms, allowing compounds to bind more firmly to the packing material surface. As a result, it can withstand strong cleaning and can be completely cleaned up. Boron bases show high selectivity for compounds with cis-diol groups, such as catechols, nucleic acids, low molecular weight proteins, and carbohydrates. PBA has been proven to be particularly effective for the separation of catecholamines from biological fluids and for the separation of RNA from DNA. It can also be applied to cis-amino alcohols like propanol. When functional groups of compounds are present on the same surface, the retention capacity becomes strong. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut PCB is designed to extract a wide range of polychlorinated biphenyls (PCBs) from biological and aqueous matrices. Bond Elut PCB is a multi-phase cartridge filled with a charge-polarized surface packing material in a super clean 3mL polypropylene tube. The optimized method for Bond Elut PCB allows for the quantitative recovery of a wide range of PCB compounds through a simple single solvent elution procedure. In an analytical example using Bond Elut PCB and CP-Select 28/31 GC capillary columns, a wide range of PCB compounds, including PCB 28 and 31, can be separated and isolated in half the time compared to previous methods. Additionally, rapid analysis with Rapid-MS PCB can significantly enhance investment efficiency. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut PH phenyl packing material is generally used for the extraction of non-polar compounds and has a polarity similar to C8. Like cyclohexane, PH shows slightly different selectivity compared to non-polar packing materials. This selectivity arises from the electron density of the aromatic ring. The retention capacity of planar conjugated organic compounds becomes stronger compared to the aliphatic compound binding phase. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut PPL, a styrene-divinylbenzene polymer, was developed to extract highly polar substances from large volumes of water samples. The high-purity spherical polymer Bond Elut PPL, with its unique coated non-polar surface functional groups, can retain even compounds classified as polar, such as phenols, providing clean extracts, high recovery rates, and rapid extraction speeds. Its extreme hydrophobicity and surface area of 600 m²/g ensure reproducibility, quantitative retention capacity, and the potential for high recovery rates. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut PRS is a highly hydrophilic strong cation exchange material, and there are no notable hydrophobic interactions observed. In non-polar solvents, it exhibits properties of hydrophilic interactions and hydrogen bonding. Due to the low pKa of PRS, cationic compounds must elute either by using a high ionic strength buffer or by suppressing the charge to isolate the cations. Therefore, PRS is effective for weak cations such as pyridine compounds. With relatively few hydrophobic secondary interactions, combined with its low capacity, PRS offers a unique set of selectivity features. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut PSA is similar to NH2. The anion exchange resin PSA (1.4 meq/g) offers a slightly higher ion exchange capacity compared to NH2 (1.1 meq/g) due to its two amine groups, and the pKa of its primary and secondary amine groups is higher (10.1 and 10.9, respectively). Therefore, PSA is a slightly stronger anion exchanger than NH2. The PSA functional group has very good bidentate ligand properties, making PSA an excellent packing material for chelation. Higher carbon loading compared to NH2 packing materials promotes hydrophobicity and makes it a good choice for compounds with high polarity that are strongly retained by NH2 packing materials. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut SAX is a strong anion exchange sorbent because its functional group is a quaternary amine, which is always charged. The effect of the carbon atoms in the functional group is masked by the amine, resulting in reduced hydrophobic interactions of SAX. Since SAX is a strong anion exchanger, it serves as a good sorbent for retaining weak anions, such as carboxylic acids, which may not be strongly held by weak anion exchangers. The counterion is stable in the form of chloride. Its selectivity can be altered by adjusting with an appropriate buffer for use. *For more details, please refer to the PDF materials or feel free to contact us.*

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Bond Elut SCX is a strong cation exchange resin with a very low pKa similar to PRS. However, due to the presence of benzene rings on the SCX surface, it has a higher potential for hydrophobic interactions compared to PRS. When using the packing material as an ion exchange in an aqueous solvent matrix, it is necessary to take into account the hydrophobic nature of SCX. This duality is beneficial for compounds that exhibit both cationic and hydrophobic properties. The interactions resulting from this dual nature are superior to purifications that use only a single binding phase. *For more details, please refer to the PDF document or feel free to contact us.*

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Bond Elut Silica is a polar packing material. Non-bonded active silica exhibits acidity. SI is a packing material used for the highly selective separation of compounds with similar structures. To elute target compounds present in a non-polar solvent, you can increase the polarity of the solvent by adding a polar solvent such as THF or ethyl acetate, allowing for recovery. *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut CBA is a silica-based medium-polarity packing material with carboxypropyl groups, which exhibits hydrophilic/hydrophobic interactions depending on the matrix and solvent environment. Its main characteristic lies in weak cation exchange. Due to its pKa value of 4.8, CBA does not require the extremely high alkaline environment that other cation exchange packing materials need for elution. At pH 4.8 and above, most of the functional groups carry a negative charge as cation exchange sites. Lowering the pH to 2.8 neutralizes CBA, allowing it to release and elute the compounds it has retained from the packing material. CBA is particularly suitable when dealing with very strong cationic compounds. Additionally, CBA broadens the selectivity range for counterions compared to other cation exchangers, increasing the options for elution buffers. *For more details, please refer to the PDF document or feel free to contact us.*

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