JNC List of Products and Services

ε-polylysine (hereinafter referred to as Polylysine) is a homopolymer consisting of 25 to 35 units of the amino acid L-lysine, where the amino group at the ε position and the carboxyl group at the α position are linked by amide bonds. It has over 30 years of manufacturing history and is widely used as a food preservative derived from amino acids. Bread making involves many processes, but if the dough can be frozen after shaping, it allows for the omission of steps prior to secondary fermentation, significantly reducing the time required for thawing, fermentation, and baking. However, freezing the dough can lead to the destruction of yeast cells and damage to the dough structure due to ice crystal formation, resulting in a decrease in volume. This volume reduction is one of the freezing injuries of bread dough. Polylysine is a safe fermented food that has been used as a preservative for many years and has the function of suppressing freezing injuries in bread. Bread with added Polylysine bakes up fluffier compared to bread without it. 【Features】 ■ A safe material produced through fermentation by natural microorganisms (not genetically modified organisms) ■ Provides a positive image as a natural fermentation product ■ Listed in the existing additive registry as a food preservative ■ A safe domestic product

• Shelf life enhancer

Streptococcus pneumoniae is one of the major pathogens causing high levels of morbidity and mortality from pneumonia worldwide, particularly in children and the elderly. Pneumococcal vaccines based on the capsular polysaccharide (CPS), one of the most important virulence factors, are used to prevent these infections. By using a two-step chromatography purification process for the purification of capsular polysaccharides, it is possible to obtain high-purity vaccines. This process employs hydrophobic interaction chromatography (HIC) and anion exchange chromatography (AEX). We introduce two optimized chromatography media for this process: Cellufine MAX Butyl HS and Cellufine MAX Q-hv. This document provides a detailed explanation of the product features and practical examples related to the chromatography purification of pneumococcal vaccines. We encourage you to read it. *For more details, please refer to the PDF document or feel free to contact us.*

• Chromatographic resins and packing materials

ε-Polylysine (hereinafter referred to as Polylysine) is a homopolymer consisting of 25 to 35 units formed by the amine group at the ε-position and the carboxyl group at the α-position of the amino acid L-lysine, linked by amide bonds. It has over 30 years of manufacturing experience and is widely used as a food preservative derived from amino acids. By adding Polylysine, the growth of microorganisms that cause food poisoning can be prevented. Every year, food poisoning caused by microorganisms is a significant issue and is frequently reported in the news. Even foods that appear to be fresh can experience rapid microbial growth if the conditions are right, so caution is necessary. Even if the appearance is good, contamination by bacteria can progress, posing a risk of food poisoning. In addition to hygiene management during the manufacturing process and proper storage at appropriate temperatures, the effective use of the preservative Polylysine can help ensure food safety more effectively. 【Features】 ■ A safe material produced through fermentation by natural microorganisms (not genetically modified organisms) ■ Provides a positive image as a natural fermentation product ■ Listed in the existing additive registry as a food preservative ■ A safe domestic product

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a homopolymer consisting of 25 to 35 units formed by the amine group at the ε position and the carboxyl group at the α position of the amino acid L-lysine, linked by amide bonds. It has over 30 years of manufacturing history and is widely used as an amino acid-derived food preservative. The shelf life of typical pet food is 2 to 3 years, but wet-type pet foods, such as treats, often have a shorter shelf life of only about 1 year. Methods for extending shelf life include canning, pouch processing, and converting to dry food, but these are not suitable for flavorful pet foods like wet types. Additionally, some pet owners do not refrigerate after opening. This can lead to mold growth or spoilage during storage, potentially harming the pet's health. Polylysine is a food preservative used in bento, bread, and side dishes, and it is gaining attention as a food preservative that can help prevent spoilage in pet food. 【Features】 ■ A safe material produced through fermentation by natural microorganisms (not genetically modified organisms) ■ Provides a positive image as a natural fermentation product ■ Listed in the existing additive registry as a food preservative ■ A safe domestically produced product

• Shelf life enhancer

1. ε-Polylysine A safe polyamino acid food preservative produced by natural microorganisms. It has over 30 years of manufacturing experience. In recent years, due to its high safety, it has been used in everyday products such as wet wipes, toothpaste, shampoos, hygiene materials, cosmetics, various cleaning agents, chemicals, and pharmaceuticals. It is also applied in preventing bacterial contamination during the manufacturing process of food ingredients, utilizing its high antibacterial properties, as well as in the removal of anionic substances using the characteristics of cationic substances, and as an additive in semiconductor CMP slurries. 2. MC-Media Pad A sheet-like microbial testing medium that serves as an alternative to agar media. By using the MC-Media Pad, there is no need for medium preparation like with agar media, allowing for space-saving and rapid microbial testing, significantly reducing the time required for testing. Additionally, compared to agar media, the amount of waste is reduced to about one-twentieth, contributing to the SDGs. It is a product certified by international certifications such as AOAC-PTM, AOAC-OMA, and MicroVal, and is listed in the food hygiene inspection guidelines, making it safe for use in compliance with HACCP.

• Shelf life enhancer

Cellulose resin MLP1000 DexS is a chromatography resin that is based on the next-generation cellulose resin MLP1000, with sulfated polysaccharides immobilized as ligands. It features highly cross-linked cellulose particles with a large pore diameter and interconnected pore structure. This allows for the use of the internal adsorption sites of the carrier in the purification of large molecular weight virus-like particles, demonstrating extremely high adsorption and purification capabilities. The PDF document presents examples of the purification of inactivated influenza viruses. We provide examples of high-purity purification achieved through chromatography using the cellulose carrier MLP1000 DexS. [Handling of the Development Product] Cellulose resin MLP1000 DexS is a development product. There is a possibility that its quality and name may change. We are providing a limited number of samples of this product as a development item. Companies (in pharmaceutical manufacturing, diagnostic reagents, and other manufacturing, etc.) and researchers who wish to consider this product for manufacturing purposes are requested to agree to this and, taking into account the intended use, we will provide samples. *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

Polylysine is widely used as a food additive and, thanks to its GRAS certification (Note) from the U.S. FDA, is recognized as a global standard food preservative. As of August 2, 2022, the only polylysine with FDA GRAS certification is the polylysine produced by JNC Corporation. Polylysine is a naturally occurring amino acid-derived substance produced by microbial fermentation, similar to miso, soy sauce, yogurt, and other products. Polylysine ionically adsorbs to microorganisms, inhibiting their growth. Due to its broad antibacterial properties and excellent flavor-preserving properties, it is primarily used to extend the shelf life of foods, and is used in a wide range of food products, including ekiben (boxed lunches), soraben (boxed lunches sold at supermarkets), and prepared meals. Note: GRAS (Generally Recognized as Safe) is a safety standard certification given to food additives by the U.S. Food and Drug Administration (FDA). To be generally recognized as safe through scientific procedures, quantitative and qualitative scientific evidence equivalent to that required for approval of a substance as a food additive is required. *For more information, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereafter referred to as Polylysine) is a substance officially known as ε-Poly-L-lysine. It has over 30 years of manufacturing history since 1989 and is widely used as a preservative in food. Polylysine is a natural food preservative produced through fermentation. Due to its high safety, we have received numerous requests to use it for various antibacterial applications. In response to customer feedback, we have started selling it as a reagent. The reagent can be purchased from Fujifilm Wako Pure Chemical Corporation. For example, it is expected to reduce synthetic polymers in everyday products closely related to daily life, such as wet wipes, toothpaste, shampoo, and scalp care. 【Features】 ■ Safe natural microbial fermentation product (not genetically modified organism) ■ Provides a positive image as a natural fermentation product ■ Listed in the existing additive inventory as a food preservative ■ Can be used as an additive in cosmetics, hygiene materials, and daily necessities ■ A safe domestic product *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

Cellufine Phosphate is an affinity chromatography resin that has an affinity for nucleic acid-binding proteins. T7 RNA polymerase can be efficiently purified using Cellufine Phosphate due to its nature as a nucleic acid-binding protein. T7 RNA polymerase is an RNA synthesis enzyme used to produce mRNA from DNA templates. In the manufacturing process of mRNA vaccines, which became famous as a vaccine for the novel coronavirus disease (COVID-19), T7 RNA polymerase is used as the enzyme for mRNA synthesis. T7 RNA polymerase can be purified to high purity through a two-step purification process using Cellufine Phosphate and Cellufine MAX DEAE (a weak anion exchange support). *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group of the essential amino acid L-lysine and the α-carboxyl group. Since 1989, it has over 30 years of manufacturing experience and is widely used as a food preservative amino acid. It was found that the combination of Polylysine and natto extract suppresses the infection of feline calicivirus (NCV) in cells. Since Polylysine and natto extract do not show infection suppression effects when used alone, the combination of Polylysine with other bioactive substances can provide new added value to Polylysine. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group and the α-carboxyl group of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing experience and is widely used as a food preservative amino acid. By blending Polylysine into synthetic resins, antibacterial resins can be obtained. Additionally, it has been reported that using dispersants allows for antibacterial effects to be achieved with a smaller amount of Polylysine added. We introduce the antibacterial effect against E. coli of polypropylene sheets blended with Polylysine. Polypropylene sheets with added Polylysine showed antibacterial effects against E. coli. By using zinc stearate as a dispersant, antibacterial effects can be achieved with a smaller amount of Polylysine added. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group of the essential amino acid L-lysine and the α-carboxyl group. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. By uniformly dispersing Polylysine in aqueous emulsion resin, an antibacterial paint can be obtained that has low environmental impact from volatile organic compounds (VOCs) and high safety for human health. This introduces the antibacterial effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis of polypropylene sheets coated with polyvinyl butyral resin dispersed with Polylysine. Sheets coated with polyvinyl butyral resin containing Polylysine exhibit antibacterial effects that depend on the amount of Polylysine in the coating film against each microorganism. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group and the α-carboxyl group of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative. Efforts are actively being made to impart the excellent antibacterial effects of Polylysine to various materials such as textiles, paints, and resin products. By immersing fibers in a Polylysine solution, it is possible to obtain antibacterial fibers with high safety for the human body. We will introduce the antibacterial effects against Staphylococcus aureus and Klebsiella pneumoniae after washing various fibers treated by immersion in Polylysine. Various fibers treated by immersion in a Polylysine solution showed antibacterial effects even after washing. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group of the essential amino acid L-lysine and the α-carboxyl group. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. Chelating agents are commonly used in food, cosmetics, various cleaning agents, chemicals, and pharmaceuticals as metal ion sequestrants and antioxidants. It is well known that chelating agents such as EDTA and sodium citrate exhibit antibacterial properties and have been used for antibacterial purposes for a long time. We present the minimum inhibitory concentration (MIC) against Escherichia coli and Staphylococcus aureus when using Polylysine in combination with EDTA. The growth inhibition effect against each microorganism is synergistically enhanced by the combination of Polylysine and EDTA. *For more details, please download the PDF or feel free to contact us.*

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group and the α-carboxyl group of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. Polylysine has excellent antibacterial properties on its own, but when added to food, its effectiveness may not be fully realized due to the influence of food components. Therefore, when used in food, it can be combined with other substances to mitigate the effects of food components or enhance its antibacterial effects. In particular, combining Polylysine with substances that suppress microorganisms through different mechanisms, such as ethanol, organic acids, glycine, and glycerol fatty acid esters, can yield excellent synergistic effects. [Content] ■ Combination with ethanol ■ Combination with organic acids ■ Combination with glycine ■ Combination with glycerol fatty acid esters *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the amino group at the ε position and the carboxyl group at the α position of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing experience and is widely used as a food preservative. Here, we introduce the safety of Polylysine. Polylysine has been confirmed to have high safety through acute toxicity, subacute toxicity, chronic toxicity, mutagenicity, and pharmacokinetic tests in accordance with the guidelines of the Ministry of Health, Labour and Welfare. 【Content Overview】 ■ Acute toxicity test (rats) ■ Carcinogenicity test (rats) ■ Mutagenicity test (microorganisms) ■ 3-month repeated dose toxicity test (rats) ■ Teratogenicity and reproductive test (rats) ■ Chronic toxicity/carcinogenicity combined test (rats) *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group and the α-carboxyl group of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. It demonstrates inactivation effects against various microorganisms. Polylysine not only suppresses the growth of microorganisms but also has the effect of inactivating them. It similarly shows inactivation effects against enterohemorrhagic Escherichia coli O157:H7 and O111:H8. From the perspective of suppressing food spoilage, it is important to keep the initial bacterial count low, and Polylysine, which inactivates microorganisms and reduces the initial bacterial count, has excellent properties as a preservative. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance officially known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group of the essential amino acid L-lysine and the α-carboxyl group. Since 1989, it has over 30 years of manufacturing history and is widely used as a preservative in food. We will introduce the effects of heat treatment on the antibacterial effect of Polylysine. The antibacterial effect of Polylysine does not change even after heating at 120°C for 2 hours. Since food is often subjected to heating during processing, Polylysine, which has excellent thermal stability, can be suitably used in heat-requiring foods. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereinafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group of the essential amino acid L-lysine and the α-carboxyl group. Since 1989, it has over 30 years of manufacturing experience and is widely used as a preservative in food. We present the minimum inhibitory concentration (MIC) of Polylysine in combination with sorbic acid, acetic acid, and glycine at pH 5 to 9. Polylysine exhibits stable antibacterial effects at low concentrations across a wide pH range from weakly acidic to weakly alkaline. Many foods we consume have a pH close to weakly acidic to neutral. Polylysine, which shows stable antibacterial effects in small amounts across a wide pH range, can be suitably utilized in various foods. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereafter referred to as Polylysine) is a substance formally known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the ε-amino group and the α-carboxyl group of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. We will introduce the minimum inhibitory concentration (MIC) of Polylysine against various microorganisms. Polylysine exhibits antibacterial effects at concentrations ranging from a few ppm to several hundred ppm, regardless of the type of bacteria, yeast, or mold. *For more details, please download the PDF or feel free to contact us.

• Shelf life enhancer

ε-Polylysine (hereafter referred to as Polylysine) is a homopolymer consisting of 25 to 35 linked ε-amino groups of L-lysine, an essential amino acid for humans, and α-carboxyl groups through amide bonds. With over 30 years of manufacturing experience, it is an amino acid-derived preservative widely used as a food preservative, making it applicable in sanitary materials and more. For example, it is expected to reduce synthetic polymers in everyday items closely related to daily life, such as wet wipes, toothpaste, shampoo, and scalp care products. Even in the presence of chelating agents used as antioxidants in cosmetics, various detergents, chemicals, and pharmaceuticals, it exhibits excellent antibacterial properties. Additionally, it is utilized in preventing bacterial contamination during the manufacturing process of food raw materials, removing anionic substances by leveraging the characteristics of cationic polymers, and in applications for electronic materials and semiconductor manufacturing. 【Features】 ■ A safe material produced through fermentation by natural microorganisms (not genetically modified organisms) ■ Provides a positive image as a natural fermentation product ■ Listed in the existing additive inventory as a food preservative ■ Can be used as an additive in cosmetics, sanitary materials, and daily necessities ■ A safe domestic product ■ A natural cationic polymer

• Shelf life enhancer

E-Polylysine(hereinafter referred to as Polylysine) is a substance officially known as ε-Poly-L-lysine. It is a homopolymer consisting of 25 to 35 units linked by amide bonds, formed from the amino group at the ε position and the carboxyl group at the α position of the essential amino acid L-lysine. Since 1989, it has over 30 years of manufacturing history and is widely used as a food preservative amino acid. As a product of natural microbial fermentation, it can reduce the use of synthetic food preservatives derived from chemical substances. Polylysine is a cationic polymer with a positive charge. It electrically binds to negatively charged microbial cell membranes, inhibiting microbial growth. Polylysine is listed in the existing additive inventory of the Ministry of Health, Labour and Welfare (No. 397). Example of labeling: Preservative (Polylysine). **Features** - Safe natural microbial fermentation product (not derived from genetically modified organisms) - Provides a good image as a natural fermentation product - Listed in the existing additive inventory as a food preservative - Usable in various foods such as bento, side dishes, various processed foods, bakery products, and confectionery - A safe domestic food product *For more details, please download the PDF or feel free to contact us.*

• Shelf life enhancer

"Cellufine SPA-HC" is a chromatography resin in which highly cross-linked spherical cellulose particles are immobilized with a highly alkaline-resistant Protein A ligand. It features excellent flow characteristics, low Protein A elution levels, and high dynamic binding capacity. Additionally, it maintains good adsorption activity even after multiple cycles of cleaning in place (CIP). This document provides a detailed explanation of "performance and features," including comparisons of dynamic binding capacity with various commercially available resin and comparisons of DBC for mAb and pAb, using diagrams and graphs. We encourage you to read it. [Contents (partial)] ■ Excellent dynamic binding capacity (DBC) ■ Comparison of adsorption amounts of monoclonal antibodies ■ Purification of monoclonal antibodies from CHO cell culture samples ■ High-throughput screening of elution pH ■ CIP stability of Cellufine SPA-HC *For more details, please refer to the PDF document or feel free to contact us.

• Chromatographic resins and packing materials

"Cellufine" is a chromatography resin that has various ligands (functional groups) modified on the surface of spherical porous cellulose particles. It is used in the production of biopharmaceuticals such as antibody drugs, vaccines, and protein formulations. This document presents an example of high-purity purification from CHO cell culture supernatant using Cellufine in a three-step process. We encourage you to read it. [Contents (partial)] ■ Purification procedure ■ Protein A chromatography process (Cellufine SPA-HC) ■ Virus inactivation by acid treatment ■ Anion exchange chromatography process (Cellufine MAX Q-h) ■ Cation exchange chromatography process (Cellufine MAX GS) *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

This document is the technical data sheet for the affinity chromatography resin "Cellufine Sulfate" handled by our company. It can be used for the purification of viruses, virus-derived antigen proteins, serum proteins, and heparin-binding proteins. It provides detailed explanations of purification examples for influenza virus, rabies virus, and endotoxin removal (depyrogenation). We encourage you to read it to assist in the process design of biopharmaceuticals. [Contents (partial)] ■ Features ■ Benefits ■ Examples of virus purification with proven adsorption and virus-derived/bacterial-derived antigens ■ Example of rabies virus purification ■ Example of influenza virus purification *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

"Cellufine Sulfate" is an affinity chromatography resin that has binding activity to viruses and serum proteins. It can be used for the purification of viruses, virus-derived antigen proteins, serum proteins, and heparin-binding proteins. This document presents a case study of the purification of influenza virus derived from chicken egg culture. By passing a near-neutral culture solution through it and increasing the salt concentration, the virus can be eluted, allowing for simple and efficient purification and concentration of the virus. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Chromatographic resins and packing materials

"Cellufine Sulfate" is an affinity chromatography resin. It can be used for the concentration and purification of viruses, removal of pyrogens from viruses, purification of virus-like particles (VLPs), and purification of proteins with affinity activity, such as bacterial-derived antigens and serum-derived proteins. This document includes flow packing procedures and examples of packing columns with inner diameters of 3.2 cm, 30 cm, and 45 cm. We encourage you to read it. [Contents (partial)] ■ Flow packing procedures ■ Example of packing a 3.2 cm inner diameter column ■ Example of packing a 30 cm inner diameter column ■ Example of packing a 45 cm inner diameter column ■ Results and discussion *For more details, please refer to the PDF document or feel free to contact us.

• Chromatographic resins and packing materials

"Cellufine ET Clean" is a chromatography resin that immobilizes ε-polylysine on cellulose particles. By strongly adsorbing endotoxins present in the sample solution onto the resins, it enables the removal of endotoxins from the sample. This document provides a detailed explanation of the product features and examples of endotoxin removal using graphs. We encourage you to read it. [Contents (partial)] ■ A new chromatography resin for endotoxin removal ■ Chemical structure ■ Product features ■ Selectivity for endotoxins (LPS) from bovine serum albumin (BSA) ■ Endotoxin removal performance from various proteins *For more details, please refer to the PDF document or feel free to contact us.

• Chromatographic resins and packing materials

This document is a technical data sheet published about the ion exchange chromatography resin "Cellufine MAX GS." The base carrier uses highly cross-linked cellulose particles, allowing for operation at high flow rates. The ligand structure is designed to efficiently separate monomers and aggregates of monoclonal antibodies. We introduce topics such as "separation of monomers and aggregates of monoclonal antibodies" and "separation characteristics of model proteins." We encourage you to read it. [Contents (partial)] ■ About Cellufine ■ Cellufine MAX GS ■ Dynamic binding capacity of Cellufine MAX GS ■ Separation of monomers and aggregates of monoclonal antibodies ■ Flow rate characteristics of Cellufine MAX GS *For more details, please refer to the PDF document or feel free to contact us.

• Chromatographic resins and packing materials

"Cellufine MAX IB" is a new mixed-mode resin used for the purification of monoclonal antibodies (Mab) after the purification of Protein A resin. By surface modification of polyallylamine and modifying some of it with butyl groups, it has the characteristic of protein adsorption even at high salt concentrations. This document introduces the basic features and chemical stability. Please take a moment to read it. [Contents (partial)] ■ Flow rate characteristics of Cellufine MAX IB ■ Cellufine MAX IB with excellent salt resistance ■ Recommended cleaning solution (CIP) ■ Mab purification after Protein A chromatography ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

Cellufine offers a range of ion exchange chromatography resins. Each resin has its own characteristics, and it is important to select the resin that is suitable for the purification of the target protein. This document introduces the features of each resin sold as Cellufine cation exchange chromatography. We encourage you to read it. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

In the downstream (purification process) of monoclonal antibody (mAb) manufacturing, it is common to use chromatography resin for the Protein A column capture step and the polishing step, which is called impurity removal. This document introduces a method that connects two columns used in the polishing step and employs them in flow-through mode. By using this method, we were able to efficiently remove impurities without the cumbersome operations of adjusting pH and electrical conductivity between the polishing process columns. [Contents] ■ Introduction ■ Experimental Methods ■ Analysis ■ Results ■ Conclusion ■ Acknowledgments *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

"Cellufine" is a chromatography resin that has various ligands (functional groups) modified on the surface of spherical porous cellulose particles. It is used in the manufacturing of biopharmaceuticals such as antibody drugs, vaccines, and protein formulations. This document reports a method to achieve high-purity purification of monoclonal antibodies through a two-step purification process using the Protein A resin Cellufine SPA-HC for the capture step and Cellufine MAX IB for the polishing step. Since it allows for high-purity purification in two steps, it can streamline the process and reduce lead times. We encourage you to read it. [Contents (partial)] ■ Overview of purification ■ Protein A chromatography ■ Mixed-mode chromatography ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

Due to advances in vaccines and clinical diagnostics, there is a growing demand for highly purified and concentrated large amounts of viral particles. "Cellufine Sulfate" is a chromatography carrier. This allows for the avoidance of cumbersome, time-consuming, and potentially dangerous conventional ultracentrifugation methods. In this document, we present an example of the purification of human coronavirus OC43 (hCoVOC43) using our product, which has extensive experience in virus purification. We encourage you to read it. [Contents (partial)] ■ Purification of hCoV OC43 viral particles ■ Evaluation of the virus by hemagglutination titer (HA titer) ■ Evaluation of host-derived DNA and protein amounts ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

• Other purification and extraction

The "MC-Media Pad" is a simplified microbiological culture media that serves as an alternative to agar media. It consists of a sheet-like medium layered with a media for detecting target microorganisms and a water absorption polymer, non-woven fabric. Since there is no need to prepare agar media, it enables rapid detection. The "MC-Media Pad Aerobic count" is a microbiological culture media that allows for easy and quick detection of aerobic count. It has obtained the international certification MicroVal and AOAC-OMA, AOAC-PTM and is also included in the food hygiene inspection guidelines, which are considered the standard for food microbiological testing in Japan. 【Features】 ■ Detects aerobic count ■ Easy to use as the sample spreads automatically ■ Good coloring of the colony ■ Contributes to reduced incubation time ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves easily microorganism testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" is a simplified microbiological culture media that serves as an alternative to agar media. It consists of a sheet-like medium layered with a media for detecting target microorganisms and a water absorption polymer, non-woven fabric. Since there is no need to prepare agar media, it enables rapid detection. The "MC-Media Pad Salmonella" is a microbiological culture media that allows for easy and quick detection of Salmonella. 【Features】 ■ Detects Salmonella ■ Easy to use as the sample spreads automatically ■ Good coloring of the colony ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves easily microorganism testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" is a simplified microbiological culture media that serves as an alternative to agar media. It consists of a sheet-like medium layered with a media for detecting target microorganisms and a water absorption polymer, non-woven fabric. Since there is no need to prepare agar media, it enables rapid detection. The "MC-Media Pad YM" is a microbiological culture media that allows for easy and quick detection of Yeast and Mold. Yeast form round red colonies, while mold form red colonies with indistinct spreading edges. It has obtained the international certifications MicroVal, AOAC-OMA, and AOAC-PTM. It is also included in the food hygiene inspection guidelines, which are considered the standard for food microbiological testing in Japan. This product can be used as an alternative method to ISO 21527-1. 【Features】 ■ Detection of detection of Yeast and Mold ■ Easy to use as the sample spreads automatically ■ Good coloring of the colony ■ Contributes to reduced incubation time ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves easily microorganism testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" (formerly known as Sanita-kun) is a simplified microbial detection medium that serves as an alternative to agar media. The "MC-Media Pad EC" is a microbial detection medium that allows for the easy and rapid detection of Escherichia coli and coliforms. Escherichia coli develops as blue colonies, while other coliforms develop as blue to blue-green colonies. Counting the blue to blue-green colonies gives the coliform count, while counting only the blue colonies gives the Escherichia coli count. It has obtained international certifications MicroVal and AOAC-PTM. It is also included in the food hygiene inspection guidelines, which are considered the standard for food microbiological testing in Japan. This product can be used as an alternative method for ISO 4832 (coliforms) and ISO 16649-2 (Escherichia coli). 【Features】 ■ Detection of Escherichia coli and coliforms in different colors ■ Easy to use as it spreads naturally with a pipette ■ Medium optimized for good bacterial coloration ■ Contributes to reduced incubation time ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves simple microbial testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" (formerly known as Sanita-kun) is a simplified microbial detection medium that serves as an alternative to agar media. It consists of a sheet-like medium layered with a medium for detecting target microorganisms and a water-soluble polymer non-woven fabric. This allows for rapid detection without the need to prepare agar media. The "MC-Media Pad SA" is a microbial detection medium that allows for easy and quick detection of Staphylococcus aureus. It has obtained international certifications MicroVal and AOAC-PTM. Additionally, it complies with the food hygiene inspection guidelines, which are considered the standard for food microbiological testing in Japan. This product can be used as an alternative method to ISO 6888-1. 【Features】 ■ Detects Staphylococcus aureus in 24 hours ■ Easy to use as it spreads naturally with a pipette ■ The medium is optimized for good bacterial coloration ■ Contributes to reduced incubation time ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves simple microbial testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" (formerly known as Sanita-kun) is a simplified microbial detection medium that replaces agar media. It consists of a sheet-like medium layered with a medium for detecting target microorganisms and a water-soluble polymer non-woven fabric. This allows for rapid detection without the need to prepare agar media. The "MC-Media Pad CC" is a microbial detection medium that allows for easy and quick detection of coliform bacteria. It has received international certifications MicroVal and AOAC-PTM. It is also included in the food hygiene inspection guidelines, which are considered the standard for food microbiological testing in Japan. This product can be used as an alternative method to ISO 4832. 【Features】 - Detects coliform bacteria within 24 hours - Easy to use as it spreads naturally with a pipette - The medium is optimized for good bacterial coloration - Contributes to reduced incubation time - Different color tapes for each microorganism help minimize operator errors - Enables simple microbial testing in compliance with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

The "MC-Media Pad" (formerly known as Sanita-kun) is a simplified microbial detection medium that replaces agar media. It enables rapid detection as there is no need to prepare agar media. The "MC-Media Pad ACplus (R-AC)" is a microbial detection medium that allows for easy and quick detection of aerobic bacteria. Thanks to its nutrient components for microorganisms and a uniquely developed specific redox indicator, it can be used in both standard mode for 48 hours and rapid mode for 24 hours. It is listed in international certifications such as AOAC-PTM, AOAC-OMA, MicroVal, and the guidelines for food microbiological testing in Japan, which are recognized as food hygiene inspection guidelines. It is also applicable to methods compliant with ISO4833. 【Features】 ■ Detects general bacteria in 24 hours ■ Easy to use as it spreads naturally with a pipette ■ Optimized medium for good bacterial coloration ■ Contributes to reduced incubation time ■ Different color tapes for each microorganism help minimize operator errors ■ Achieves simple microbial testing compliant with HACCP *For more details, please download the PDF or feel free to contact us.

• Microbiology

Cellufine MAX GS is a second-generation chromatography resin that allows for use at higher flow rates. By utilizing surface modification technology, it can be used at high flow rates while also possessing high dynamic binding capacity. It can be used as a high-performance chromatography resin, which is expected to enhance downstream processing capacity. Cellufine MAX GS is a new strong cation exchange chromatography resin optimized for ligand concentration. It demonstrates excellent performance in removing aggregates from monoclonal antibodies. 【Features】 - Can be used for polishing purification of antibody drugs - Capable of separating antibody dimers and aggregates - Unique salt tolerance that allows protein adsorption even at high salt concentrations - Excellent flow rate characteristics due to advanced crosslinking technology - Contributes to the reduction of the amount of support used due to high adsorption capacity *For more details, please refer to the PDF document or feel free to contact us.

• Chromatography

"Cellufine MAX IB" is a new mixed-mode resin used for the polishing purification of monoclonal antibodies (Mab) after the purification of Protein A resin. It features surface modification with polyallylamine and the modification of some parts with butyl groups, allowing proteins to adsorb even at high salt concentrations. 【Features】 ■ Can be used for the polishing purification of antibody drugs ■ After Protein A purification, it can achieve high purity with just one purification step ■ Contributes to reduced lead times and cost savings through a two-step purification process ■ Stable resin that allows for in-place cleaning with 0.5M sodium hydroxide ■ Unique salt tolerance that retains proteins even at high salt concentrations *For more details, please refer to the PDF document or feel free to contact us.

• Chromatography

Cellufine ET Clean is a chromatography resin that immobilizes poly-ε-lysine on spherical cellulose particles. It can efficiently remove endotoxins from the target sample solution. Cellufine ET Clean can effectively remove endotoxins produced by gram-negative bacteria under conditions such as physiological saline. Since endotoxins do not elute even under high salt concentration conditions, it can be suitably used when endotoxins need to be removed from proteins. The product is available in two types: Cellufine ET Clean S: Small pores, resulting in less non-specific adsorption of proteins. Cellufine ET Clean L: Large pores, capable of adsorbing a large amount of endotoxins. 【Features】 - Adsorbs endotoxins at high salt concentrations. - At high salt concentrations where endotoxins are adsorbed, proteins pass through. - Efficiently removes endotoxins from proteins and other substances. - Stable polyamino acids do not become inactive, allowing the use of harsh cleaning solutions. *For more details, please refer to the PDF document or feel free to contact us.

• Chromatography

Cellufine Sulfate is an affinity chromatography resin used for virus purification. It has a proven track record of use in vaccine manufacturing by global pharmaceutical companies worldwide. The density gradient ultracentrifugation method used for virus purification has several drawbacks, including lead time, reproducibility, and safety issues. Cellufine Sulfate can address these shortcomings due to its chromatography capabilities. 【Features】 ■ Affinity activity for live viruses, inactivated viruses, and split viruses ■ Affinity for virus-derived antigens, bacteria-derived antigens, and heparin-binding proteins ■ The chromatography process is closed, ensuring high safety and low risk of microbial contamination ■ Effective in removing endotoxins that may have contaminated the manufacturing process, as it does not adsorb endotoxins ■ Due to its negatively charged ligands, it does not adsorb positively charged impurities such as host-derived DNA ■ Physically robust and highly durable as it is based on cellulose ■ Sterilization through multiple autoclave cycles is possible *For more details, please refer to the PDF materials or feel free to contact us.

• Chromatography