ナニオンテクノロジーズジャパン List of Products and Services

We are pleased to announce that Nanion Technologies Japan Co., Ltd. will co-host the Membrane Physiology Symposium Japan 2025 in collaboration with Professor Hideaki Kato's laboratory at the University of Tokyo's Advanced Research Center. Following the symposium, we will also hold a modest networking event, and we warmly invite you to participate. For details on the program and other information, please refer to the related links below. Speakers (in alphabetical order): - Dr. Kenji Akiyoshi | Keio University, Faculty of Pharmacy - Dr. Shuutaro Katsurabayashi | Fukuoka University, Faculty of Pharmacy - Dr. Hideaki Kato | University of Tokyo, Advanced Research Center - Dr. Tomoya Kubota | Osaka University Graduate School of Medicine - Dr. Shin Hamamoto | University of Tokyo Graduate School of Agricultural and Life Sciences - Dr. Yuichiro Miyaoka | Tokyo Metropolitan Institute of Medical Science - Dr. Chai-Ann Ng | Victor Chang Cardiac Research Institute - Dr. Alexandre Santinho | Oria Bioscience - Dr. Will Seibertz | Nanion Technologies GmbH - Dr. Martin Ziller | Nanion Technologies GmbH

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You can experience actual measurements and demonstrations using our various products. For more details, please see the related links below. *Capacity: Approximately 10 to 20 people *Due to limited lab space, depending on the number of participants, there may be a possibility of dividing into two sessions, morning and afternoon. *Participants who register for the workshop will be contacted again once the details are finalized.

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**Catalog Products** ■ Auto Patch Clamp System Ideal for evaluating the effects of compounds on ion channels and for functional and structural analysis of ion channels. From a compact system that allows you to check data immediately at hand to high-throughput systems for compound screening in pharmaceutical research and development. ■ Artificial Lipid Bilayer Experimental Device For functional and structural evaluation of target membrane proteins reconstituted into artificial lipid bilayers, as well as for evaluating drug actions. ■ Liposome Preparation Device Quickly and easily automates the preparation of liposomes without hassle. ■ Transporter Activity Measurement Device Enables label-free and real-time direct measurement of transporter currents without using fluorescent probes or radiolabeled ligands. ■ iPS-Derived Cardiomyocyte Measurement System For assessing cardiotoxicity, contractility, and drug screening using iPS-derived cardiomyocytes. ■ Cell Monitoring Device Allows for label-free and real-time high-throughput measurement of cell kinetics (up to 6 x 96-well plates).

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We have published examples of cell and ion channel measurements using our auto patch clamp system. * For more details, please refer to the PDF materials from the "Catalog" at the bottom of the page. * Please feel free to contact us for requests regarding product and application case introductions. * Application cases are continuously added and updated.

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This is a document summarizing the differences in required equipment and measurement principles between the traditional manual patch clamp method and the automated patch clamp system. *For more details, please refer to the PDF document or feel free to contact us.*

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Designed to seamlessly integrate fully automated compound screening into the drug discovery process, it provides the best performance in high-throughput screening of ion channels in drug discovery, pharmacology, safety, pharmaceutical research and development, and exploratory screening departments. Additionally, by using the 32-well mode, it can be optimized for small-scale compound screening and projects. ■ Simultaneous measurement of 384 cells ■ 20,000 data points per day ■ High giga-seal success rate ■ Supports a variety of ion channel targets and enables rapid assay system development ■ Measurement of action potentials using current clamp is also possible ■ Extracellular/intracellular perfusion is possible ■ High-speed external solution exchange (up to 110 µl/s) is possible ■ Measurement section and 12 decks can be individually temperature controlled (temperature range: 10–37°C) ■ Compound plate preparation on the deck is possible ■ Single & multi-well chips available (in-house manufactured) ■ Borosilicate glass chips reduce compound adsorption ■ Approximately 8 hours of unmanned operation is possible ■ Comes with powerful analysis software ■ Validated system for CiPA

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This is a patch clamp system of the world's smallest class that can perform gigaseal recordings, optimal for safety testing/toxicity testing/pharmacological testing/screening of compounds at the cellular level in the functional structural analysis of ion channels, research on related diseases, and drug development processes. 【Features】 ■ No need for manual patch clamp experience or difficult operations ■ No need for microscope, vibration table, manipulator, Faraday cage, or puller ■ 20 to 50 data points per day ■ Automation from gigaseal formation to whole cell formation ■ Compatible with voltage-dependent, ligand-dependent, and temperature-dependent channels ■ Options available for research on channel rhodopsins and mechanosensitive channels using light stimulation ■ Measurement of action potentials using current clamp is also possible ■ Supports a wide range of applications and enables rapid assay system development ■ Reduces compound adsorption with borosilicate glass chips ■ Optional temperature control (10 to 50°C), capable of extracellular/intracellular perfusion ■ Can be integrated with amplifiers such as HEKA and Axon *For details, please refer to the catalog available for download in PDF.

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This is a world-class patch clamp system capable of performing gigaseal recordings, optimal for safety testing/toxicity testing/pharmacological testing/screening of compounds at the cellular level in the functional structural analysis of ion channels, research on related diseases, and drug development processes. ■ No experience with manual patch clamping or difficult operations required ■ No need for microscope/vibration isolation table/manipulator/Faraday cage/puller ■ 20 to 50 data points per day ■ Automation from gigaseal formation to whole cell formation ■ Compatible with voltage-dependent and ligand-dependent channels through pipetting ■ Measurement of action potentials using current clamp is also possible ■ Supports a wide range of applications, enabling rapid assay system development ■ Reduces compound adsorption with borosilicate glass chips

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This is a tabletop-sized auto patch clamp system that can simultaneously measure up to 8 cells with giga-seal formation. With easy experimental setup, stable whole-cell recordings, and sophisticated software, it enables highly efficient compound ion channel screening. ■ Equipped with a dispensing device, allowing for fully automated experiments (measurements) ■ Simultaneous measurement of 4 cells / 8 cells (up to 48 cells can be measured continuously) ■ 250 to 600 data points per day ■ Automation from giga-seal formation to whole-cell formation ■ Compatible with voltage-dependent and ligand-dependent channels ■ Measurement of action potentials using current clamp is also possible ■ Dynamic clamp capability (optional) ■ Temperature control and extracellular/intracellular perfusion possible ■ Cooling plate allows for cooling of cells and compounds ■ Borosilicate glass chips reduce compound adsorption ■ Comes with excellent analysis software

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■ External Standard: 500ml ■ External Standard Ca 10: 500ml ■ External [-] Ca2+ [-] Mg2+: 500ml ■ External NMDG 60: 500ml ■ External NMDG 60: 500ml ■ Internal CsF 110: 500ml ■ Internal KF 110: 500ml ■ Washing Solution for Tips: 5L Certificates of Analysis, Material Safety Data Sheets (MSDS), included.

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【Measurement Target Examples】 Ion channels (voltage-dependent, ligand-dependent, temperature-sensitive), DNA nanopores, antimicrobial peptides, toxins, etc. In experiments using artificial lipid bilayers, the formation of lipid membranes, reconstitution of membrane proteins, and measurement of single-channel currents can be easily performed. Purified target membrane proteins (such as ion channels in vivo or artificial ion channels) can be reconstituted on artificially formed lipid bilayers, allowing for the evaluation of drug effects and functional/structural analysis. ■ Simultaneous formation and measurement of 4 channels of artificial lipid bilayers is possible ■ Lipid membranes can be formed with just pipetting operations ■ No need for microscope, vibration isolation table, manipulator, or Faraday cage ■ Direct reconstitution of target membrane proteins or membrane fusion with proteoliposomes ■ Temperature control (10–50°C) available (optional) ■ Palm-sized, easy connection via USB

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【Measurement Target Examples】 Ion channels (voltage-dependent, ligand-dependent, temperature-sensitive), DNA nanopores, antimicrobial peptides, toxins, etc. Experiments using artificial lipid bilayers allow for easy formation of lipid membranes, reconstitution of membrane proteins, and measurement of single-channel currents. It is possible to reconstitute purified target membrane proteins (such as ion channels found in vivo or artificial ion channels) on artificially formed lipid bilayers for drug action evaluation and functional/structural analysis. By rapidly and automatically forming 16-channel artificial lipid bilayers at once and conducting fully simultaneous measurements, researchers are freed from the burdens of the painting method in experiments using artificial lipid bilayers. ■ Simultaneous formation and measurement of 16-channel artificial lipid bilayers is possible ■ Automatic lipid membrane formation using a stirrer bar ■ No need for microscope, vibration isolation table, manipulator, or Faraday cage ■ Direct reconstitution of target membrane proteins or membrane fusion with proteoliposomes ■ Temperature control available (10–50°C)

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The electroformation method (vibrating hydrated lipid films in an alternating electric field) allows for the production of giant unilamellar vesicles (GUVs) with diameters ranging from 1 to 30 μm, free of organic solvents, with high yield and reproducibility. With flexible protocol design and temperature control, GUVs can be created from a variety of lipids (such as DPhPC, DPPC, DOPC, POPC), including charged lipids and those with high phase transition temperatures. *For operating procedures, please refer to the "Related Links" below. *For more details, please check the PDF materials or feel free to contact us.

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Currently, the mainstream method for evaluating transporter activity is the uptake assay using RI-labeled substrates. However, there are several issues, such as the need for specialized experimental facilities, the complexity of waste liquid treatment, the fact that the desired substrates are not always commercially available as RI-labeled, and the measurement of endpoints after uptake. SURFE²R (Surface Electrogenic Event Reader) technology does not require RI labeling, specialized experimental facilities, or waste liquid treatment, and it allows for real-time evaluation of the activity of transporters (symporters, exchangers, unipoters) and pumps. ■ Direct measurement of transporter currents using Solid Supported Membrane (SSM) ■ Experiments can be conducted using membrane fragments prepared from biological membranes or proteoliposomes reconstituted with the target membrane proteins ■ Light stimulation is possible (optional) ■ An all-in-one device that integrates a dispensing machine, measurement section, and computer ■ 150 data points per day

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Currently, the mainstream method for evaluating transporter activity is the uptake assay using RI-labeled substrates. However, there are several issues, such as the need for specialized experimental facilities, complicated waste disposal processes, the fact that the desired substrates are not always commercially available as RI-labeled, and that the endpoint is measured after uptake. The SURFE²R (Surface Electrogenic Event Reader) technology does not require RI labeling, does not need specialized experimental facilities or waste disposal, and allows for real-time evaluation of the activity of transporters (symporters, exchangers, unipoters) and pumps. ■ Direct measurement of transporter currents using Solid Supported Membrane (SSM) ■ Experiments can be conducted using membrane fragments prepared from biological membranes or proteoliposomes reconstituted with the target membrane proteins ■ Simultaneous measurement in 96 wells ■ 10,000 data points per day

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Can you provide the list of validated targets for the transporter activity measurement device SURFE²R? *For more details, please refer to the PDF document or feel free to contact us.*

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Measurement Example: Investigating TMEM175 with the SURFE2R 96SE *For more details, please refer to the PDF document or feel free to contact us.

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This is a hybrid cardiotoxicity screening device capable of impedance measurement and extracellular potential measurement similar to MEA. It can detect not only cardiomyocytes but also the minute contractions of cancer cells and liver cells due to the effects of compounds. ■ Simultaneous measurement of impedance and extracellular potential ■ 96-well simultaneous measurement ■ Label-free measurement ■ Long-term monitoring capability ■ Electrical pacing & optical pacing (optional) ■ Comes with an incubation system (temperature, humidity, and CO2 control) ■ Includes multifunctional and excellent data analysis and graph creation software ■ Validated human iPS cells: iCell, iCell², AXOL CM, Cardiosight-S, MiraCell Cardiomyocytes, etc. ■ Compact desktop size: W20.5 x D18 x H11.7cm / 3.5kg (main unit)

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The FLEXcyte 96, which transforms the conventional Langendorff method into the latest high-throughput technology, can measure the contractile force (mN/mm²) of cardiomyocytes for cardiotoxicity and safety evaluation, as well as drug screening, under physiological conditions similar to the native cardiac tissue environment, without missing even slight responses to cytotoxicity, regardless of whether in the chronic or acute phase, simultaneously in 96 wells. ■ Simultaneous measurement in 96 wells ■ Label-free measurement ■ Long-term monitoring possible ■ Optical pacing (optional) ■ Comes with an incubation system (temperature, humidity, CO2 control) ■ Includes multifunctional excellent data analysis and graphing software ■ Validated human iPS cells: iCell, iCell², AXOL CM, Cardiosight-S, MiraCell Cardiomyocytes, etc. ■ Compact desktop size: W20.5 x D18 x H11.7cm / 3.5kg (main unit)

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Measurement of Contraction Force in Cardiomyocytes Using FLEXcyte96 The FLEXcyte membrane plate features a silicon membrane with a thickness of less than 10μm at the bottom of a 96-well plate. When cardiomyocytes are seeded onto the plate, the weight of the culture medium causes the silicon membrane to deflect, and subsequently, dynamic deflection (up and down movement) occurs due to the synchronized beating of the cardiomyocytes. By measuring the changes in deflection with our proprietary Capacitive Distance Sensor, developed in collaboration with innoVitro, it is possible to calculate mechanical stress and measure the actual contraction force under physiological conditions.

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【Application Examples】 CAR-T cell killing assays, cell proliferation (viability), cytotoxicity (damage), biological barrier function, cell signaling, GPCR measurements, etc. AtlaZ can measure and analyze the impedance changes of cells in up to 6 x 96-well plates in real-time. Since it allows for daily kinetics measurements while remaining in the incubator, a single assay can yield a wealth of data regarding cell responses. Additionally, by changing the measurement frequency range, it can be applied to various applications. ■ Simultaneous measurement of up to 6 x 96-well plates (independent measurements are also possible) ■ Daily kinetics measurements are possible ■ A wide measurement range through frequency range adjustments | Frequency range (0.1 kHz to 100 kHz) ■ Selectable measurement modes (TEER, Cell Monitoring, Cytolysis mode) (Measurements with different functions for each plate are also possible) ■ Management of measurement plates is possible with the included barcode scanner.

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A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes

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Application Note: High throughput APC recordings from RealDRGTM iPSC sensory neurons for analgesia drug discovery *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Pharmacology of P2X3 and P2X2/3 receptors in cell lines and hiPSC-derived neurons: An automated patch clamp study *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: High throughput organellar electrophysiology of TMEM175 and TPC2 from freshly isolated lysosomes recorded on the SyncroPatch 384 *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Pharmacology of transient receptor potential cation (TRP) channels using different activation stimuli *For more details, please refer to the PDF document or feel free to contact us.*

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A high-throughput electrophysiology assay to study the response of PIEZO1 to mechanical stimulation.

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Unique electrophysiological property of a novel Nav1.7, Nav1.8, and Nav1.9 sodium channel blocker, ANP-230

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Application Note: Properties and Pharmacology of NaV1.5-ΔKPQ Late INa Current on the SyncroPatch 384i *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Biophysical modulation of hHCN2 by bPAC recorded on the SyncroPatch 384PE *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Activation and Inhibition of Human ASIC3 Channels on Nanion’s SyncroPatch 384PE *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Effect of temperature on erythromycin action on hERG currents recorded on Nanion's Patchliner *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Pharmacology of hNaV1.5 recorded on Nanion's Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Characterization of rNaV1.8 (ND7-23) on Nanion's Patchliner *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Fully automated dynamic clamp for the Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Heat activation of TRPV3 on Nanion’s Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Nicotinic α3β4 receptors recorded on Nanion's Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Activation, potentiation, and inhibition of AMPA receptors on the Patchliner *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Investigating PIEZO1 using the Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: High throughput APC recordings from RealDRGTM iPSC sensory neurons for analgesia drug discovery *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: P2X2/3 receptors recorded on Nanion's Patchliner *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Recordings of Action Potentials in Mouse ES Cell-Derived Cor.At Cardiomyocytes on Nanion's Port-a-Patch *For more details, please refer to the PDF document or feel free to contact us.*

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Application Note: Identification of Cardiac Liability in Drug Discovery Using the Port-a-Patch *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: Patch clamp recordings of hNaV1.7 on Nanion’s Port-a-Patch *For more details, please refer to the PDF document or feel free to contact us.

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Application Note: TRPV1 and TRPM8 recorded on Nanion's Port-a-Patch *For more details, please refer to the PDF document or feel free to contact us.

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