サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K. List of Products and Services

The "Accucore HPLC column" is a column that can be used with almost all HPLCs. Due to its low back pressure, it can be used with general-purpose HPLCs, and its high peak capacity allows for the separation and identification of more compounds in a single analysis. A wide variety of stationary phases is a feature of this product, including some very unique stationary phases. It provides a solution for compounds that could not be retained or separated with existing stationary phases. 【Features】 ■ Developed using core technology, stationary phase manufacturing technology, and packing technology ■ Usable with almost all HPLCs ■ An even richer variety of stationary phases ■ Allows for the separation and identification of more compounds in a single analysis *For more details, please refer to the PDF materials or feel free to contact us.

The "pH Gradient Buffer" enables the development of analytical methods for charge variants contained in proteins and monoclonal antibodies (MAbs) through LC, as well as reducing the time required for method transfer to QA/QC. Thanks to the characteristics of the buffer components, a fast and reproducible pH gradient is achievable. Additionally, the development of analytical methods is simple and easy. Furthermore, it can be used with LC columns and systems commonly used for biomolecular analysis, and there is no time-consuming preparation of the mobile phase as with gradients using salts. 【Features】 ■ Achieves a fast and reproducible pH gradient due to the characteristics of the buffer components ■ Development of analytical methods is simple and easy ■ Usable with LC columns and systems commonly used for biomolecular analysis ■ No time-consuming preparation of the mobile phase as with gradients using salts ■ Methods using pH gradients are applicable to most MAbs

The "DNAPac PA200 HPLC/DNAPac PA200 RS (High-Speed Separation) UHPLC Column" is a column designed for the high separation of ssDNA and RNA. The selectivity for oligonucleotides can be controlled by various parameters such as the pH of the mobile phase, solvents, and the use of different salts, allowing for adjustments in separation to meet the specific requirements of applications. We provide structural analysis and quantification methods utilizing high-resolution mass spectrometry and anion exchange columns for UHPLC. 【Features】 ■ High separation of oligonucleotide chains and stereoisomers ■ Improved separation and throughput ■ Control of selectivity and retention through the pH of the mobile phase, type of salt, and type of mobile phase ■ Separation of n from n-1 bases *For more details, please refer to the PDF document or feel free to contact us.

The "Accucore XL HPLC Column" is a column filled with 4μm solid core particles utilizing advanced core technology. It surpasses the performance obtained from general-purpose HPLC methods using fully porous packing materials of 5μm, 4μm, and 3μm. With very high separation efficiency even under normal HPLC systems and analytical conditions, it achieves excellent separation and lower detection limits. Thanks to high-precision packing technology, it is a robust column with good retention and excellent reproducibility. 【Features】 ■ Highly compatible with standard HPLC methods ■ No need to reconsider methods or purchase new equipment ■ High separation capability allows for the separation of many peaks in challenging analyses ■ Sharp and high peak shapes improve detection limits ■ Good reproducibility provides reliable data *For more details, please refer to the PDF document or feel free to contact us.

The "GlycanPac AXR-1 column" is a high-performance silica-based HPLC column that separates glycans based on charge, structural isomerism, and size. It can achieve high separation of biologically important glycans with unique selectivity, regardless of labeling. Additionally, analysis using fluorescence detectors and mass spectrometers is possible. 【Features】 ■ Unique selectivity based on charge, structural isomers, and size ■ Excellent separation capability for both labeled and unlabeled glycans ■ Utility of structural analysis and quantification of glycans based on high separation capability ■ Method compatibility with mass spectrometers and fluorescence detectors ■ Reliable column performance ■ Particle sizes for HPLC (3μm) and UHPLC (1.9μm) *For more details, please refer to the PDF document or feel free to contact us.

The "Acclaim Trinity P2 Column" is a high-performance silica-based column. It is particularly suitable for HPLC analysis of counter ions in charged pharmaceuticals. The usage is simple, it boasts excellent robustness, and it allows for the analysis of both monovalent and multivalent ions using fast general-purpose methods. Using a general-purpose HPLC, it is possible to effectively analyze counter ions with just one column and one type of analytical method. 【Features】 ■ Effectively analyzes ions using HPLC ■ Enables screening of counter ions present in pharmaceuticals ■ Retains ions or ionic compounds without using ion-pair reagents ■ Simple to use ■ Provides reproducible packing *For more details, please refer to the PDF materials or feel free to contact us.

The "Accucore HPLC Column for Biomolecules" is a filler column with a pore size of 150A, highly suitable for the analysis of biomolecules. Based on excellent core technology, it enables high separation and fast analysis. The solid core particles with a pore size of 150A used in the Accucore150-C18 and 150-C4 columns are designed to provide an optimal combination for the retention and separation of peptides and proteins. The Accucore150-C4 column achieves sharper and higher peaks compared to a 5μm wide-pore fully porous C4 column, resulting in better separation and sensitivity, while also reducing back pressure. 【Features】 ■ Solid core particles that enable fast and high-separation analysis ■ Stable quality through automatic column packing ■ High-efficiency columns due to strictly controlled particle size distribution ■ Advanced chemical modification technology that produces high coverage and a robust stationary phase *For more details, please refer to the PDF document or feel free to contact us.

The "MAbPac HIC-Butyl column" is a high separation hydrophobic interaction chromatography (HIC) column made from a polymer substrate. It is specifically designed to separate mAbs and ADCs (antibody-drug conjugates). Additionally, it reduces sample adsorption to the packing material by binding butyl groups to a hydrophilic substrate at an appropriate density, which helps suppress carryover and provides selectivity that complements the "MAbPac HIC-10 column" and "MAbPac HIC-20 column." 【Features】 ■ Selectivity suitable for DC analysis ■ High separation and high-efficiency analysis ■ Stable over a wide pH range (2-12) ■ Low carryover ■ Reproducible column packing *For more details, please refer to the PDF document or feel free to contact us.

The "Acclaim Trinity P1 Column" is a silica-based high-resolution column designed for the simultaneous analysis of pharmaceutical formulation ingredients and their counter ions, as well as for the simultaneous analysis of acidic, basic, and neutral pharmaceuticals. The innovative Nano Polymer Silica Hybrid (NSH) technology provides unparalleled chromatography performance and excellent flexibility in adjusting selectivity during method development. 【Features】 ■ Multi-retention mechanism through reversed phase, anion exchange, and cation exchange ■ Adjustability of selectivity by changing ionic strength, buffer type, pH, and organic solvent composition ratio ■ Recommended for simultaneous analysis of basic, neutral, and acidic components ■ Retains ions and ionic components without the use of ion-pairing agents *For more details, please refer to the PDF document or feel free to contact us.

The "DNAPac RP Column" is a reverse phase (RP) liquid chromatography column developed for the analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC/UV or LC/MS. Thanks to its unique column technology, it demonstrates excellent performance across a wide range of pH, temperature, and mobile phase compositions. Additionally, the wide-pore resin allows for effective separation of large double-stranded nucleic acids up to 10,000 base pairs. 【Features】 ■ For ion-pair reverse phase (IP-RP) separation of oligonucleotides and double-stranded nucleic acids ■ High resolution and throughput ■ Excellent MS compatibility ■ Wide operating pH range (0-14) and high temperature stability (up to 100°C) *For more details, please refer to the PDF document or feel free to contact us.

The "ProPac Ion Exchange Column" is an ion exchange column for the characteristic analysis and quality evaluation of protein mutations. It achieves high efficiency and high separation for proteins and glycoproteins (isoelectric point (pI) = 3 to 10; molecular weight (MW) > 10,000). Additionally, the non-porous pericellular type packing material enables the separation of proteins that differ by just one charge, and due to the minimal hydrophobic interactions with the packing resin, it provides exceptionally good peak shapes. 【Features】 ■ Achieves high efficiency and high separation for proteins and glycoproteins ■ Designed to meet regulatory requirements for the characterization of biopharmaceuticals ■ High reproducibility of the packing material eliminates variability between columns during method development and data analysis ■ Four types of ion exchange resins with different selectivities ■ Unique packing technology *For more details, please refer to the PDF document or feel free to contact us.

In "Antibody Analysis and Characterization of Antibodies: New Solutions," we cover terms related to biopharma, as well as charge variant analysis and more. Additionally, we introduce solutions for the characterization of monoclonal antibodies (mAb) and biopharmaceutical analysis. [Contents] ■ Terms related to biopharma ■ Introduction - Characterization of monoclonal antibodies (mAb) ■ Workflow for biopharmaceutical development ■ Quantification by affinity ■ Aggregate separation *For more details, please refer to the PDF document or feel free to contact us.

The "TraceGOLD GC Column" is a column that excels in stability at high temperatures, has low bleed, and offers a long lifespan. We offer a range of columns from general non-polar columns to polar columns, including the "traceGOLD TG-1 MS GC Column," which is non-polar, has extremely low bleed, and achieves suitable S/N ratios, sensitivity, and mass spectra, as well as the "TraceGOLD TG-5SilMS GC Column," which is low-polarity with excellent inertness. 【Features (traceGOLD TG-1 MS GC Column)】 ■ Non-polar ■ Equivalent to USP G2 ■ Low bleed ■ Achieves suitable S/N ratios, sensitivity, and mass spectra *For more details, please refer to the PDF document or feel free to contact us.

The "GlycanPac AXH-1 column" is a filler column based on silica that separates glycans based on charge, size, and polarity. This product is designed for the analysis of biologically significant glycans, enabling high separation and high-throughput analysis. It possesses unique selectivity, allowing for the analysis of glycans with or without labeling using fluorescence detectors or liquid chromatography-mass spectrometry (LC-MS) systems. 【Features】 ■ Unique selectivity based on charge, size, and polarity ■ High separation of both labeled and unlabeled glycans ■ Characterization and quantification of glycans based on their charge ■ Capable of fluorescence detection and MS detection ■ High efficiency and excellent column robustness *For more details, please refer to the PDF document or feel free to contact us.

The "Acclaim Surfactant Plus Column" is a state-of-the-art HPLC column with high efficiency and silica-based material. It allows for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants. It can be used for the analysis of consumables such as detergents, pharmaceuticals, food and beverages, and environmental samples. 【Features】 ■ Selectivity suitable for the simultaneous analysis of cationic surfactants, anionic surfactants, nonionic surfactants, and amphoteric surfactants ■ Measurement of cationic surfactants ■ Separation of ethoxylated surfactants ■ Retention of highly hydrophilic compounds such as hydrotropes *Please feel free to contact us for more details.

The "Reacti-Therm" is a revolutionary system that combines the Thermo Scientific Reacti-Ther heating/stirring module and the Thermo Scientific Reacti-Vap evaporator, allowing for heating, stirring, and concentration drying all in one device. Additionally, it allows for easy exchange of aluminum blocks compatible with various test tubes, microcentrifuge tubes, and vials. - Derivatization of HPLC and GC samples - Hydrolysis of proteins - Small-scale reactions - Incubation - Hydrolysis of amino acids - Evaporation For more details, please refer to the PDF materials or feel free to contact us.

The "Practical Guide to Microelution SPE" covers the advantages and specifications of solid-phase extraction (SPE) "SOLAμ" using Embedded Particle Technology (EPT), which requires less filling material, as well as starting points for SOLAμ extraction and troubleshooting. Additionally, it introduces other considerations regarding ion exchange methods, as well as the calculation of SPE recovery rates and matrix effects. [Contents] ■ What are the advantages of SOLAμ Microelution SPE? ■ SOLAμ selectivity ■ Specifications ■ Scaling down from established methods to micro SPE ■ Concentrating large samples to achieve low quantitation limits *For more details, please refer to the PDF document or feel free to contact us.

The "SMART Digest Kit" is a kit that allows for the sample pretreatment of proteins. Thanks to the heat-stable immobilized trypsin design, proteins can be digested quickly, simply, and reproducibly for characterization and quantification applications. Additionally, the sample preparation process is simplified, reducing the required time. This not only accelerates the workflow but also significantly shortens method development time. There is also the "SMART Digest ImmunoAffinity (IA) Kit," designed for the quantification of biomarkers. 【Features】 ■ Fast ■ Simple ■ High reproducibility ■ High sensitivity ■ Automation compatible *For more details, please refer to the PDF materials or feel free to contact us.

The "SOLA SPE Cartridge and Plate Technical Guide" outlines the features of the solid phase extraction (SPE) "SOLA," which enables cleaner and more consistent recovery without frits, achieving excellent reproducibility. It also includes advantages compared to conventional SPE. Additionally, we introduce general extraction procedures and product information for SOLA. [Contents] ■ Technical Information ■ Improvement of Reproducibility and Recovery Rate ■ Increased Sensitivity and Reduced Solvent Consumption ■ Clean Extraction *For more details, please refer to the PDF document or feel free to contact us.

Well plates offer many useful tools to automate the filling, handling, and sealing of samples, making them equipment that can be expected to provide high throughput. However, they are often made of polypropylene (PP), which poses issues such as limitations on usable solvents, contamination of samples due to leachables from the plate caused by solvents, and loss of compounds due to adsorption on the well walls. The Thermo Scientific WebSeal Plate+ addresses these issues by coating the walls with glass, while also retaining the advantages of plastic well plates (such as automation with robots and the use of multi-pipettes).

pH has a significant impact on the ionization state of specific analytes. For example, a base with a pKa of 8.5 is 99% ionized at pH 6.5 using the 2 pH unit rule and 99% non-ionized at pH 10.5. When the analyte is close to its own pKa, unstable chromatograms may be observed, so it is necessary to adjust the pH to achieve the appropriate ionization state. Differences in the surface activity of vial glass and the leaching of hydrolysis products that affect the sample pH can be confirmed by monitoring the pH changes of deionized water stored in the vial. To investigate how the quality of glass, represented by the expansion coefficient, affects the pH of the solution, we measured the fluctuations in the pH of 18 MΩ pure water stored in an autosampler vial for a certain period at room temperature and compared the results with those obtained using polypropylene containers as a control.

Thermo Scientific Acclaim columns are ideal for high-efficiency high-performance liquid chromatography (HPLC) separations. Their unique features enable challenging and complex separations, allowing for the separation of compounds that traditional HPLC columns cannot achieve. The Thermo Scientific Acclaim columns utilize standard surface chemistry features, including standard functionalities such as 120 C18, C8, Phenyl, Polar Advantage, polar embedded phases, HILIC, as well as mixed-mode functionalities (Thermo Scientific Acclaim Trinity P1 and P2 columns), and application-specific phases (such as organic acids, surfactants, pesticides, contaminants, etc.), along with specialized columns like size exclusion columns (SEC). Furthermore, some Acclaim columns are optimized to operate under the ultra-high-performance liquid chromatography (UHPLC) requirements of the Thermo Scientific Vanquish UHPLC system.

Thermo Scientific SOLA products are designed for bioanalysis and clinical research analysts with the mission of providing high-quality analytical results from complex biological samples in high-throughput environments while adhering to strict regulations. Such needs continue to drive the push for more effective drugs and long-acting formulations, lowering the sensitivity requirements for accurate quantification to even lower levels. To meet these needs, bioanalytical methods must have the following: • Robustness – low analytical failure rate • Ability to process low-volume samples • High sensitivity • High reproducibility • Ease of use • High-throughput processing • High-efficiency and fast processes

Adeno-associated virus (AAV) is a suitable vector for many gene therapies. The Empty/Full capsid ratio of AAV is an extremely important quality characteristic that needs to be monitored throughout the entire development cycle. The Thermo Scientific ProPac column series leverages approximately 20 years of experience in ion exchange to continue supporting researchers in the biotherapeutics field. The Thermo Scientific ProPac SAX-10 LC column easily separates the Empty/Full capsid bodies of AAV serotype 6 and AAV serotype 8 in less than 10 minutes, allowing for immediate quantification results with minimal sample pretreatment.

Liquid chromatography aims to achieve good separation ability to obtain meaningful information through peak resolution. In mixed-mode chromatography, numerous parameters can be adjusted to optimize separation efficiency, allowing for effective control of separation performance. The Thermo Scientific Acclaim mixed-mode column features unique surface chemistry that facilitates easy adjustment of analyte elution, enabling maximum separation efficiency, which often leads to successful separation of compounds that cannot be addressed by other conventional high-performance liquid chromatography (HPLC) columns. The combination of ion exchange and reversed-phase, specifically HILIC chemistry, can extract the chemical properties of sample mixtures, allowing for complete separation of components in complex samples or high-sensitivity detection of trace samples. However, it is impossible to retain highly polar or charged analytes using only reversed-phase chromatography. Ion exchange (IEX) chromatography alone is insufficient for separating hydrophobic compounds. By using columns that combine different functionalities, a completely new toolkit is obtained for separating and evaluating samples.

Solid-phase extraction (SPE) is a highly effective modern bioanalytical technique for isolating analytes from complex biological matrices. It improves accuracy and precision, enables detection at very low levels, and reduces matrix effects that can be problematic in mass spectrometry.

Thermo Scientific SOLAμ solid phase extraction (SPE) well plates are designed for researchers engaged in bioanalysis and clinical studies that require cleaner, more reproducible, and robust sample extraction with very low amounts of sample and solvent in high-throughput workflows. The SOLAμ well plates achieve this with their unique innovative fritless SPE technology. The SOLAμ well plates are advanced micro-elution products that faithfully meet the requirements of bioanalysts.

Thermo Scientific SOLA products bring a major revolution to solid phase extraction (SPE). With frit-free SPE, cleaner and more consistent recoveries are achievable, resulting in excellent reproducibility. Compared to traditional phospholipid removal plates and protein removal plates, SOLA products demonstrate unparalleled superior performance. Key features: • Improved reproducibility • Enhanced extract purity • Reduced amount of organic solvent used • Increased sensitivity

The HPLC-CAD system analyzes choline and its counterions, as well as impurities of choline, with high precision without the need for derivatization. The analysis conditions using mixed-mode with hydrophilic interaction chromatography (HILIC) accommodate both charged particle detection and mass spectrometry, enabling the simultaneous analysis of anions and cations, which allows for rapid and reliable detection of choline content and impurities. In this application note, we successfully detected the content of choline derivatives such as choline chloride, choline bitartrate, and choline citrate, along with their impurities, with high precision. Choline chloride, which had poor responsiveness with conventional UV detectors, can also be directly detected without the use of derivatization methods. Furthermore, the mixed-mode using HILIC accurately identifies impurities while avoiding interference from counterions. This serves as a powerful tool for ensuring safety and efficacy in quality control for dietary supplements and other products.

Foods containing sugar often have a high content of sucrose, making it difficult to accurately quantify sucrose and other disaccharides (such as lactose and maltose) in actual samples due to insufficient separation. This technical document adopts a single-loop heart-cut 2D-LC method, where lactose and maltose are fractionated in the sample loop after sucrose elutes in the first dimension, allowing for the separation and analysis of lactose and maltose in the second dimension, thereby avoiding the influence of sucrose. Additionally, the charged aerosol detector (CAD) used in the detection allows for gradient analysis, providing greater flexibility in separating contaminants in a variety of food samples, enabling efficient analysis. This achieves high-sensitivity analysis that was difficult with conventional refractive index detectors (RI). 【Features】 - Accurate analysis by separating lactose and maltose in foods containing sugar - Separation from contaminants possible with the single-loop heart-cut 2D-LC method - Charged aerosol detector (CAD) allows for gradient analysis and accommodates diverse samples - Achieves high-sensitivity analysis that is difficult with conventional refractive index detectors (RI)

This technical document introduces a rapid and quantitative analytical method for the simultaneous detection of seven types of vitamins (vitamin A, vitamin D2, vitamin D3, and four types of vitamin E isomers) in infant formula using a conventional HPLC system with a diode array detector and a fluorescence detector, following offline solid-phase extraction (SPE) and two-dimensional liquid chromatography (2D-LC) analysis. By changing the traditional liquid-liquid extraction method to solid-phase extraction, the time required for sample preparation was reduced from 3 hours to 1 hour. Additionally, by simplifying the sample preparation and optimizing the separation conditions, the influence of the matrix was reduced, allowing for the detection of target substances with higher sensitivity. ■ Simplified sample preparation through two-dimensional liquid chromatography (2D-LC) analysis ■ Simultaneous separation analysis of vitamins A, D, E, and their isomers ■ Developed a method with higher recovery rates and sensitivity than liquid-liquid extraction ■ Simplified the preprocessing process and reduced analysis time by 2.5 times ■ Reduced the influence of the matrix and provided reliable data

TD-GC-MS/MS is a system suitable for the analysis of volatile PFAS in the air. TD concentrates the target substances from hundreds of liters of air and connects to a high-sensitivity triple quadrupole GC-MS/MS to detect concentrations in pg/m3. Since PFAS exist in low concentrations in the air, the system requires a low detection limit. Additionally, because PFAS are widely used in various products, it is crucial to ensure there is no contamination from the system or components. Evaluating background levels is essential for obtaining reliable analytical results. The technical documentation for PFAS analysis in indoor air presents examples of screening analysis using full scan and high-sensitivity analysis using target SRM for 19 PFAS components emitted from indoor air and everyday products. It evaluates the emissions from indoor air in diverse workplace spaces and everyday items. Methods for measuring PFAS released from solid samples, such as everyday products, without pre-treatment are also introduced. The technical documentation for PFAS analysis in the atmosphere introduces high-throughput analysis of neutral and ionic PFAS. It also shares know-how for improving analytical accuracy using samples collected from three locations in an industrial park.

The Charged Aerosol Detector (CAD) is a highly versatile liquid chromatography detector capable of detecting all non-volatile and semi-volatile compounds. In particular, it can sensitively detect compounds that do not absorb UV light, such as lipid nanoparticles (LNPs). Additionally, the consistent responsiveness of the CAD allows for accurate assessment of the relative amounts of each lipid component. Furthermore, it is suitable for monitoring impurities and degradation products, in addition to evaluating the composition of LNPs. This application note presents examples of constructing a fast and robust UHPLC method using the Thermo Scientific Accucore C30 LC column to separate and evaluate lipid components contained in two different formulations, as well as an example using the Thermo Scientific Hypersil GOLD C8 column to effectively profile lipid components and their impurities present in raw materials. For this analysis, we used the bio-compatible Thermo Scientific Vanquish Flex and Thermo Scientific Vanquish Horizon UHPLC systems.

Many amino acids do not absorb ultraviolet (UV) light, making it difficult to improve sensitivity and selectivity with conventional analytical methods, which are also complicated and lack stability. This technical document introduces a case where a custom injection program feature in the auto-sampler of the Thermo Scientific Vanquish Core HPLC system was utilized to derivatize 20 types of amino acids simultaneously using a pre-column derivatization method for simultaneous analysis. This system enables highly accurate quantitative analysis as it is not affected by the degradation after the derivatization reaction. Validation using commercially available beverages showed that selective quantification was possible even in complex sample matrices such as food. 【Features】 ■ Simultaneous derivatization and analysis of 20 types of amino acids ■ High-precision quantitative analysis achieved through pre-column derivatization ■ Provides good linearity and high reproducibility ■ Selective detection even in complex sample matrices ■ Automation by the auto-sampler avoids human error and improves work efficiency

This technical document introduces a post-column analysis system for accurately quantifying organic acids in various beverages. The analysis column uses ion exclusion mode, and by adding BTB solution post-column, it separates and detects ten types of organic acids with good reproducibility. The e-workflow feature, which supports operations in routine analysis, is a significant advantage as it fully automates the process from device startup to measurement and report generation. The HPLC system features a compact design that incorporates a dual pump with two pumps in one module for analysis and post-column solution delivery. Additionally, even in the event of an error, the automatic switching of valves prevents the post-column reaction solution from backflowing into the analysis column, allowing for safe adoption in routine analysis. 【Features】 ■ Accurate quantification of ten types of organic acids with good reproducibility of less than 1% ■ Separation of organic acids using ion exclusion mode columns, allowing measurement without matrix effects ■ Detection limits for organic acids in this method range from a few to 7 μg/mL ■ Automation of the process from device startup to report generation with the e-workflow feature ■ Ensured safety with automatic valve switching in case of errors

This technical document presents a case study of the simultaneous analysis of polyphenols contained in beverages and health foods with high sensitivity, unaffected by other components. A 1.5 µm solid-core particle column was used for the analysis, separating 36 types of polyphenols under gradient conditions within 10 minutes. By using standard samples of each polyphenol, calibration curves and detection limits were confirmed, achieving good linearity with a coefficient of determination of over 0.997 and detection limits in the low ng/mL range. Additionally, in the spiking recovery tests conducted on multiple health foods and beverages, approximately 100% recovery was obtained, confirming accurate quantification without interference from food components. This system reliably separates and detects components with similar structures, such as polyphenols, providing reduced analysis time and high reproducibility. Furthermore, its compact design and ease of operation streamline routine analyses. 【Features】 ■ Simultaneous analysis of 36 polyphenol components ■ High separation using a 1.5 µm solid-core particle column ■ Detection limits in the low ng/mL range and high sensitivity ■ Good calibration curves with a coefficient of determination over 0.997 ■ Accurate quantification with approximately 100% recovery, unaffected by food components

This technical document presents a case study on the highly sensitive quantification of eight components, including monosaccharides, disaccharides, and sugar alcohols, in beverages using the Thermo Scientific ISQ EC single quadrupole mass spectrometer. There are various methods for sugar analysis using HPLC, but traditional differential refractive index detectors have challenges such as sensitivity issues and the inability to perform gradient analysis. Additionally, while fluorescence detectors can detect low concentrations, they require derivatization, making the sample preparation complicated. The ISQ EC single quadrupole mass spectrometer enables the quantification of sugars at low concentrations ranging from a few to several tens of ng/mL, thanks to its high sensitivity and selectivity. Furthermore, its user-friendly device and analysis software allow easy operation for both beginners and experts in mass spectrometry, streamlining routine analyses and enabling the rapid acquisition of reliable quantitative data. 【Features】 ■ High sensitivity: Detection limit of a few to several tens of ng/mL ■ High selectivity: Accurate quantification unaffected by interfering components ■ Improved operability: No complex sample preparation required

Quantitative analysis of gloss agents (saccharin and 2-butin-1,4-diol) in electroplating nickel plating solution using LC-MS helps maintain a consistent composition of the plating solution and greatly aids in the quality control of gloss agents. This method analyzes the components contained in the gloss agents under two different conditions based on their chemical composition. Additionally, samples can be easily measured by simply diluting them with water, and good results ranging from 92% to 108% have been obtained in addition-recovery tests. Furthermore, analytical conditions have been devised to achieve stable measurements over a long period. By using MS, detection is possible even at low concentrations in samples containing high concentrations of impurities, such as plating solutions, and detection is possible with both UV and MS. Measurement of not only gloss agents but also decomposition and by-products is possible, leading to further evaluation of the plating solution. For more details, please download the materials. 【Features】 ■ Quality control of gloss agents achieved through quantitative analysis using LC-MS ■ Measurement of gloss agents and decomposition/by-products is possible ■ Analysis of gloss agent components under two different conditions ■ Samples can be easily measured by simply diluting with water ■ Analytical conditions enable stable measurements over a long period

The Thermo Scientific Vanquish Duo UHPLC system is a dual LC system equipped with two independent flow paths, allowing for parallel condition testing of different separation chemistries, such as the combination of ion exchange chromatography (IEX) and ion-pair reverse phase chromatography (IP-RP), within the same system. This technical document utilizes a method scouting kit to examine various ion-pairing agents, gradient conditions, pH, and column temperatures, achieving good peak shapes for mRNA and high-sensitivity detection of impurities. By simultaneously investigating IEX and IP-RP, it is possible to understand the separation characteristics of the sample, enabling efficient determination of separation conditions. With high separation capability and sensitivity, impurities can be effectively detected during post-transcription purification, allowing for reliable mRNA purification. **Features** - Simultaneous examination of two different separation chemistries (IEX and IP-RP) using the same device. - High-sensitivity detection of impurities in post-transcription purification through mRNA profiling. - Efficient determination of suitable separation conditions using the method scouting kit. - Examination of up to 10 solvents per column type to determine appropriate mobile phase conditions.

This document introduces a method for the high-precision separation and quantification of four types of tocopherols contained in vegetable oils using the single-loop heart-cut 2D-LC method. Under the separation conditions of a reverse-phase system using a general ODS column, the separation of β-tocopherol and γ-tocopherol is difficult; however, the 2D-LC method allows for easy separation. In the first dimension, tocopherols and interfering components in the sample are separated, and in the second dimension, the four types of tocopherols (α, β, γ, δ) are separated. The repeatability of the standard samples achieved a high reproducibility with RSD < 0.1%. Additionally, measurements conducted on multiple vegetable oils showed that quantification was possible without the influence of interfering peaks. The single-loop heart-cut 2D-LC method is the simplest technique among 2D-LC methods, making it applicable for routine analysis and a useful tool for food analysis involving samples with a high content of matrix components. 【Features】 ■ High-precision separation achieved with the single-loop heart-cut 2D-LC method ■ Separation of β-tocopherol and γ-tocopherol, which is difficult in reverse-phase systems ■ Effective separation of interfering substances in food ■ High reproducibility with repeatability of RSD < 0.1%

This technical document introduces an analytical method for sugar alcohols based on official methods. We measured six types of commercially available foods using the Thermo Scientific Syncronis Amino LC column in HILIC mode and the Thermo Scientific HyperREZ XP CarbohydrateCa2+ LC column in ligand exchange mode. Furthermore, the Thermo Scientific Chromeleon Chromatography Data System (CDS) report designer automatically calculates the sugar alcohol content in the food by inputting the sample volume, fixed volume, and dilution ratio during measurement. This helps prevent human errors and allows for the rapid and accurate creation of the final report. 【Features】 ■ High-precision measurement of sugar alcohols based on official methods ■ Use of specific columns in HILIC mode and ligand exchange mode ■ Automatic calculation and accurate result verification by the Chromeleon CDS report designer ■ High-reliability custom report function to prevent transcription errors and rounding errors *For more details, please download the PDF or feel free to contact us.

The development and quality control of LNP formulations require rapid and accurate identification of lipid components and evaluation of molar ratios. This technical document introduces a method for efficiently quantifying the concentration and molar ratio of each lipid in LNP formulations. The Charged Aerosol Detector (CAD) is a versatile detector for liquid chromatography that can detect all non-volatile and semi-volatile compounds. It is particularly sensitive for detecting compounds that do not absorb UV light, such as LNPs. Furthermore, by leveraging the CAD's high sensitivity, consistent responsiveness, and wide dynamic range, we aim to simplify sample preparation and facilitate method development. Additionally, by using the Thermo Scientific Accucore C30 LC column, we can effectively separate lipid components and other excipients in LNP formulations in a short time. This has also confirmed that the measurement results align well with the theoretical molar ratio%. 【Features】 ■ Rapid and accurate identification of lipid components and evaluation of molar ratios ■ Compliance support with Thermo Scientific Chromeleon CDS software ■ Effective separation with Accucore C30 LC column ■ Measurement results that align well with theoretical molar ratio%

We offer the 'Invitrogen PureLink RNA Mini Kit', which is suitable for high-quality, high-purity RNA extraction. It features a spin column format that eliminates the need for phenol-chloroform extraction. Despite its mini column size, it achieves yields comparable to midi kits. It can accommodate a wide range of sample volumes and types. 【Features】 ■ Purifies high-quality total RNA in about 20 minutes ■ Can obtain purified RNA up to 1,000 μg in a single extraction ■ Does not use harmful organic solvents (phenol/chloroform) in the dissolution step *For more details, please download the PDF or feel free to contact us.

This document presents guidelines for smoothly integrating the Thermo Scientific Vanquish HPLC/UHPLC system with Waters Corporation's Empower 3 Chromatography Data System (CDS), along with case studies from Contract Development and Manufacturing Organizations (CDMOs). By introducing a new LC system, we were able to significantly reduce system suitability failures (SSF), extend column life, and improve operational efficiency in a cGMP environment. Additionally, we will provide specific examples of the method transfer process between analytical instruments from different manufacturers. 【Features】 ■ Significant reduction in system suitability failures due to equipment upgrades ■ Intelligent features that greatly simplify the method transfer process ■ Achieving reproducible and robust separations with very high stability, precision, and accuracy ■ Thermo Scientific Vanquish Duo UHPLC system: significantly improved throughput and productivity with two independent flow paths

The Thermo Scientific Vanquish Split Sampler significantly reduces the effort and time associated with traditional manual dilution by allowing users to set up an automated dilution program for samples. This technical note verifies the accuracy and reproducibility of automated dilution of standard solutions through the measurement of DNPH-derivatized formaldehyde. Custom injections using user-defined programs enable easy execution of multi-step dilutions and automation of calibration curve creation. Furthermore, by utilizing the e-workflow of Thermo Scientific Chromeleon CDS, the entire process from analysis to report generation can be greatly streamlined, enhancing the reliability of analyses and increasing laboratory productivity. **Features** - High reproducibility and accuracy through automated dilution - Flexible dilution settings with custom injection programs - Automation of analysis with Chromeleon CDS e-workflow, eliminating human error - Significant reduction in effort and time compared to manual dilution - High-precision preparation capable of up to 20-fold dilution