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Pipette tip type small-scale protein purification 'PhyTip column'

Protein purification at the microscale - Ideal for small-scale multi-sample purification of antibodies, proteins, AAV, plasmids, and more.

PhyTip is a unique micro-scale chromatography that employs the patented dual-flow chromatography (DFC) technology, which is a foundational technology. It enables micro-scale purification of antibodies, AAV, proteins, and plasmids. The resin can be used from as little as 5 µl, allowing for small-scale protein purification, concentration, and high-throughput applications. Due to its chip-type chromatography, it is compatible with major liquid handlers. The use of PhyTip columns requires low-speed pipetting operations, necessitating specialized electric pipettors like the 'ME System' or liquid handler robots. It supports a wide range of resins and custom packing options. Key PhyTip column products include: ▪ Antibody purification: CaptureSelect PhyTip ProPlus LX (MabSelect SuRe) Columns, etc. ▪ AAV purification: CaptureSelect AAVX ▪ Affinity protein purification: IMAC PhyTip Columns, etc. ▪ Gel filtration ▪ Ion exchange ▪ Plasmid purification (mini prep)

Company：バイオタージ・ジャパン
Price：100,000 yen-500,000 yen

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Pipette tip type antibody purification ProPlus PhyTip column

Antibody purification on a micro-scale using a proprietary chip compatible with HTS.

This is the PhyTip column ProPlus/ProPlus LX (MabSelect SuRe) using Proplus resin, which outperforms Protein A and Protein G. ▪️ Equipped Technology It features a unique microscale chromatography that employs the patented dual-flow chromatography (DFC), which is the foundational technology of the PhyTip column. Due to its chip-type chromatography, it is compatible with major liquid handlers. The use of the PhyTip column requires pipetting operations at low speeds, so a dedicated electric pipetter, the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide range of resins and custom packing is also available. It is suitable for processing small volumes of multiple samples and can be adapted to major liquid handler robots. A dedicated electric pipetter, the 'ME System', is available for adjustments before transitioning to a smaller number of samples or liquid handlers. If you wish to use a resin not listed in the product list, we can fill the resin for you.

Company：バイオタージ・ジャパン
Price：Other

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CaptureSelect AAV PhyTip column

Achieve HTS with a small amount of purified recombinant AAV! CaptureSelect AAV Phytip column.

CaptureSelect AAVX Affinity Matrix is specially designed to meet the high selectivity and capacity required for large-scale downstream purification of a wide range of natural and synthetic adeno-associated virus (AAV) serotypes used in gene therapy applications. CaptureSelect AAVX resin has demonstrated binding reactivity against a series of AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, and synthetic serotypes. The PhyTip column is filled with a minimum resin volume of 5 µl, and its unique Dual Flow Chromatography technology allows for the complete process of protein adsorption, washing, and elution to be performed with pipetting operations. It is compatible with major liquid handlers for chip-type chromatography. The use of the PhyTip column requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. It is compatible with a wide variety of resins and custom packing is also available.

Company：バイオタージ・ジャパン
Price：Other

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Pipette tip type His-tagged protein purification PhyTip column

Comprehensive HTS from His tag purification in small amounts! IMAC PhyTip column

The IMAC PhyTip column allows for easy affinity purification in small amounts, starting from a minimum resin volume of 5 µL. If you want to process recombinant protein purification in small quantities with multiple samples, it can be handled using HTS. It is compatible with major liquid handlers for chip-type chromatography. The use of the PhyTip column requires slow pipetting operations, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It supports a wide variety of resins and custom packing is also available.

Company：バイオタージ・ジャパン
Price：Other

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Pipette tip type Strep-tag protein purification PhyTip column

For the purification of micro-scale small amounts of streptavidin fusion proteins! Streptavidin PhyTip column.

Automatic purification of biotin-tagged proteins The Streptavidin PhyTip column filled with a medium that immobilizes streptavidin affinity ligands enables the automatic affinity purification of recombinant biotin-labeled proteins. One of the attractive applications of the Streptavidin PhyTip column is the biotinylated protein assay (immunoprecipitation). It is compatible with major liquid handlers for chip-based chromatography. The use of the PhyTip column requires pipetting operations at low speeds, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide range of resins and custom packing is also available. Custom filling of the desired resin into the chip is possible. *For more details, please visit our website or feel free to contact us.

Company：バイオタージ・ジャパン
Price：Other

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Pipette tip-type small-scale GST fusion protein purification PhyTip column

For micro-scale (small quantity) GST-tagged protein purification! GST PhyTip columns are also ideal for HTS.

It can be used for protein purification of GST-tagged fusion proteins on a microscale. It is compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting at low speeds, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide variety of resins and custom packing is also available.

Company：バイオタージ・ジャパン
Price：Other

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Pipette tip type protein purification gel filtration PhyTip column

Industry-first high-throughput gel filtration PhyTip columns for desalting and buffer exchange at small scale and multiple samples.

The chip-type gel filtration PhyTip column filled with size exclusion chromatography resin is optimal for desalting protein samples and exchanging buffer, and it also enables high-throughput screening (HTS) in a 96-well plate. Desalting using the gel filtration PhyTip column can be set up as a secondary purification step after affinity purification with an affinity PhyTip column, such as the removal of imidazole after IMAC affinity purification, using the same automated protocol. Like other PhyTip columns, the resin is packed between two hydrophilic screens; however, unlike other PhyTip columns, the gel filtration column passes the sample from the top. The gel filtration PhyTip column has size exclusion resins of 5K and 30K, which retain low molecular weight compounds while allowing molecules of 5 kDa and 30 kDa or larger to pass through. Unlike other PhyTip columns, the use of the ME system is not required. Major liquid handling robots can be used for multi-sample processing. *For more details, please visit our website or feel free to contact us.*

Company：バイオタージ・ジャパン
Price：Other

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Pipette tip-type small volume ion exchange PhyTip column

Ideal for ion exchange on a microscale! Ion exchange PhyTip columns compatible with HTS.

Ion exchange chromatography is used as an orthogonal chemistry to obtain high-purity final products, serving as a second polishing step after another purification process or affinity chromatography purification. Ion exchange PhyTip columns are chip-type columns that utilize Dual Flow Chromatography technology, making them suitable for efficient parallel purification of small amounts of protein samples. They are compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. They can accommodate a wide variety of resins and custom packing is also available. Product Information (4 products): Strong Anion exchange Weak Anion exchange Strong Cation exchange Weak Cation exchange

Company：バイオタージ・ジャパン
Price：Other

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[Valve] OPUS Column

Introducing an innovative lightweight single-use valve that can be directly mounted on the OPUS prep pack column!

The "Repligen ProConnex Valve" enhances the usability and efficiency of OPUS pre-packed columns through innovative Artesyn technology. Its lightweight polymer structure allows for direct attachment to both the inlet and outlet ports. This reduces the risk of column damage and simplifies tube attachment, streamlining setup in manufacturing environments. 【Features】 ■ Lightweight polymer valve can be directly connected to OPUS columns ■ Easy to use with clear open/close indicators ■ Three ports: inlet, outlet, and bypass ■ Smooth and sealed flow control with no dead legs ■ Simple usability that does not require additional gaskets *For more details, please download the PDF or feel free to contact us.

Company：レプリジェンジャパン
Price：Other

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Inert LC Column

Eliminate data variability! Start analysis without time-consuming preparation.

Our "Inert LC Column" is a product specialized for the precise analysis of low molecular weight compounds that easily adsorb metals. There is no need for time-consuming conditioning or complex passivation processes. Reproducibility is improved, saving time, which enhances analytical accuracy and achieves high throughput. Additionally, as peak shapes are improved, there is no need for passivation or mobile phase additives, leading to increased sensitivity and recovery rates, with an expected improvement in detection limits. 【Features】 ■ Improvement of peak shape ■ Improvement of recovery rate and sensitivity ■ High accuracy and throughput ■ No conditioning required *For more details, please refer to the PDF document or feel free to contact us.

Company：Restek
Price：Other

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Restek's WAX column series

We propose the most suitable WAX type GC column for your application.

WAX-type fused silica GC columns use highly polar polyethylene glycol (PEG) as the stationary phase, enabling excellent separation of polar compounds that are difficult to achieve with non-polar or low-polarity columns. WAX columns exhibit varying heat resistance, inertness, and selectivity due to the optimization of stationary phase chemistry, allowing them to perform well for specific analytes.

Company：Restek
Price：Other

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Technical Data: Reliable Analysis of Underwater Glycol by Split Injection

Reliable analysis of glycol in water by split injection.

In the analysis of low-concentration glycols in water samples using splitless injection, it is difficult to obtain stable results. This is mainly due to issues such as backflushing, abnormal peak shapes, and shifts in retention time. The split injection method presented here can avoid these problems and ensure good chromatography results. Durability tests confirmed that the Rtx-Wax column produces stable results even after 600 water injections.

Company：Restek
Price：Other

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