Tohoku University Technology:Target specific and non-biased gene amplification: T14-107
TGene expression analysis is possible without changing copy number ratio in a sample!
In comprehensive gene expression analysis such as T cell receptor (TCR) repertoire analysis, it is important that target specific and non-biased gene amplification products are obtained. Conventional methods often ligate adapters with restriction enzyme site to synthesized cDNA ends and treat adapter-ligated cDNAs with said restriction enzyme before specific amplification. In such methods, however, unused and untreated adapters often inhibit target specific and non-biased amplification. This invention performs target specific, non-biased gene amplification by combination of uracil-containing adapter and uracil DNA glycosylase (UNG) treatment (Fig 1).
- Company:Tohoku Techno Arch Co., Ltd.
- Price:Other