[Next-Generation Sequencing Analysis Case] MLST Analysis *Case study collection available
We will introduce a case study that was able to identify strains mixed in environmental DNA!
MLST (Multi-locus sequencing typing) analysis is a method for typing bacteria by patterning mutations in multiple gene regions (usually more than seven). In this analysis case (in-house data), samples were prepared assuming the identification of isolates' genomes and environmental bacteria (samples mixed with environmental DNA and the genomes of isolated strains), and typing was conducted. The prepared strains included Gram-positive and Gram-negative bacteria, and genome sequencing and typing were performed on these samples using Miseq. As a result, this method allowed for the identification of strains mixed with environmental DNA. [Case Overview] - Samples were prepared assuming the identification of isolates' genomes and environmental bacteria, and typing was conducted. - The prepared strains were Bacillus subtilis subsp. subtilis str. 168 (Gram-positive) and Pseudomonas protegens Pf-5 (Gram-negative). - Genome sequencing and typing were performed on these samples using Miseq. *For more details, please refer to the PDF document or feel free to contact us.*
- Company:ファスマック
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