テクノスルガ・ラボ List of Products and Services

■Features - Feces, skin swabs, and saliva can be stably maintained at room temperature for one month. - A wide variety of options for intestinal environment analysis, skin microbiome analysis, oral microbiome analysis, etc. - Amplicon sequencing analysis can be performed with the collected samples. - Kits are available for analyzing organic acids (short-chain fatty acids) in feces. - The preservation solution has been confirmed to inactivate coronaviruses. - Collection containers, instruction manuals, and fecal collection sheets are individually packaged, making them easy to distribute to subjects. - For intestinal environment analysis, there are easy-to-use brush-type and spoon-type collection tools. - For skin microbiome analysis, there is a swab type that makes it easy to collect skin swabs. - For oral microbiome analysis, there is a straw-integrated design that is convenient for saliva collection. ■Free Samples Requests for free samples can be made through our inquiry form. https://www.tecsrg.co.jp/services/inquiry/ 【For more details, please check the related links】

• Other laboratory equipment and containers

● Contracted microbiome analysis of various samples such as intestinal microbiota, skin microbiota, and oral microbiota ● Amplicon sequencing analysis using next-generation sequencers (MiSeq, Illumina) ● Comprehensive support for DNA extraction, PCR, sequencing, and analysis in-house ● Options available for α and β diversity analysis, principal component analysis, cluster analysis, etc. ● Delivered data can also be used for academic papers ● Specimens can be stored at room temperature using our fecal collection kit Overview: Amplicon sequencing analysis is an excellent method for analyzing biological communities in samples containing diverse species. PCR amplification and sequencing analysis are performed using primers with barcode sequences added to the mixed DNA extracted from the samples to identify them, yielding tens of thousands to hundreds of thousands of sequences at once. A large amount of nucleotide sequences can be obtained in a single analysis, and comprehensive detection of bacteria (including fungi and yeasts), including minor bacteria, is possible. 【For more details, please check the related links】

• Contract Inspection

This is a test to analyze various components in feces. In relation to research and development in medicine, anti-aging, probiotics, etc., the intestinal environment is gaining attention. Additionally, to clarify the relationship between the intestinal environment and health, analysis of fecal components and intestinal microbiota analysis are useful. Our company offers analysis of components in the intestinal environment, such as organic acids (short-chain fatty acids), bile acids, and IgA. ■ Analysis Items ● Analysis of organic acids (short-chain fatty acids) in feces ● Analysis of bile acids in feces ● Analysis of putrefactive products in feces ● Analysis of ammonia in feces ● Measurement of fecal pH ● Measurement of fecal moisture ● Analysis of IgA in feces (human) ■ Sample Acceptance Records Feces and intestinal contents from humans, mice, rats, pigs, cows, horses, chickens, dogs, cats, and other pets. 【Please check the related links for more details】

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One method for evaluating the functionality of food is the analysis of the intestinal environment, which has garnered attention. For example, by examining organic acids (short-chain fatty acids) in feces and the intestinal microbiota, changes in the intestinal environment can be captured. Our company offers analysis services for organic acids (short-chain fatty acids) in feces and intestinal microbiota. In intestinal environment analysis, feces are often used as a non-invasive sample that can be collected. It is recommended that feces be quickly frozen using liquid nitrogen or similar methods after collection and stored at -80°C. Maintaining this frozen state is particularly one of the hurdles in sample collection for intestinal environment analysis involving humans. Therefore, our company has developed and sells a collection kit that allows for the preservation of short-chain fatty acids and intestinal microbiota without freezing the feces. [For more details, please check the related links.]

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■ Analysis of intestinal microbiota and organic acids (short-chain fatty acids) in feces can be performed without freezing. ■ The upper part of the container is filled with a preservation solution, allowing for quantitative concentration correction using internal standard substances in the preservation solution. ■ This is a kit sold in combination with the Rakuryu Cup WIDE. ◎ Available analysis items - Amplicon sequencing analysis - T-RFLP analysis - Real-time PCR analysis *Organic acid analysis is required - Organic acid analysis (short-chain fatty acids) ◎ Features - The microbiota in feces can be stably maintained at room temperature (1°C to 30°C) for about one month. - Samples can be collected even in places without freezing facilities due to room temperature storage. - A stool collection container with a spoon and a three-dimensional stool collection sheet (Rakuryu Cup WIDE) are included, making it easy for subjects to collect samples. ◎ Free samples available Requests for free samples can be made through our inquiry form on the website. Please feel free to contact us. https://www.tecsrg.co.jp/services/inquiry/

• Other laboratory equipment and containers

■Quantitative Analysis of Organic Acids (Short-Chain Fatty Acids) in Feces ● Features 1. Quantitative analysis of various organic acids contained in feces. 2. Can accept samples from a single specimen. ● Analysis Items Acetic acid, propionic acid, succinic acid, lactic acid, iso-butyric acid, iso-valeric acid, n-butyric acid, n-valeric acid, formic acid ■Analysis of Gut Microbiota ● Features 1. Analysis of the bacterial community in feces. 2. The microbiota is analyzed at the phylum to species level through amplicon sequencing analysis. ◎ Analysis items: Identification of reads, α and β diversity analysis using QIIME2, statistical analysis using R, predictive metagenomic analysis, etc. 3. Comprehensive support from DNA extraction to microbiota analysis. 4. Capable of real-time PCR analysis ◎ Target bacterial groups: Akkermansia muciniphila, Faecalibacterium prausnitzii, Bifidobacterium genus, etc. ■Room Temperature Storage Fecal Collection Kit "Metabolokeeper (Feces)" ● Features 1. Fecal samples can be stored at room temperature (1-30°C) for one month without freezing. 2. Preserves organic acids (short-chain fatty acids) and gut microbiota in feces.

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This catalog introduces the "Biologically Related Microbial Community Structure Analysis / Microbiome Analysis Related Kits" provided by Technosuruga Lab Co., Ltd., a company specializing in microbial identification contract testing services. It includes various items such as the "Bacterial Isolation Fecal Collection Kit" with preservation solution for isolating anaerobic bacteria from feces, and the simple assembly "Complete Fecal Collection Kit." [Contents] ■ Information and Common Guide ■ Sale of Biologically Related Collection Containers and Kits ■ Microbial Community Structure Analysis (Microbiome Analysis) and Physicochemical Analysis ■ Data Analysis ■ Other Microbial Tests and Products *For more details, please download the PDF or contact us.

• Food Testing/Analysis/Measuring Equipment
• Contract Analysis
• Other safety and hygiene products

This catalog introduces the "Microbial Identification Tests for Researchers" provided by Technosulga Lab Co., Ltd., a company specializing in microbial identification contract testing services. It includes various tests such as the "MALDI Microbial Identification Test (Biotyper)" that supports identification in all categories of bacteria, molds (filamentous fungi), and yeasts, as well as the "Rapid Analysis - Mold Morphology Rapid," which can report with biosafety levels for assigned species and closely related species. [Contents] ■ Introduction and Common Guide ■ <Guide> Microbial Identification Tests for Researchers ■ Rapid Identification (MALDI & Rapid) ■ Bacterial Genetic Analysis, Morphological Observation, Physiological Testing ■ Fungal Genetic Analysis, Morphological Observation, Physiological Testing, etc. *For more details, please download the PDF or contact us.

• Food Testing/Analysis/Measuring Equipment
• Other safety and hygiene products
• Contract Inspection

This catalog introduces the "Environmental Microbial Community Structure Analysis / Microbiome Analysis Kits" provided by Technosulga Lab Co., Ltd., a company specializing in microbial identification testing services. It includes various services such as "DNA extraction" to extract DNA from different types of environmental samples, and "Amplicon Sequencing Analysis (Bacteria and Archaea)" that can accommodate a wide range of samples including culture media from soil and environmental water, as well as fermented foods. [Contents] ■ Introduction and Common Guide ■ Microbial Community Structure Analysis (Microbiome Analysis) ■ Data Analysis ■ Other Microbial Tests and Products *For more details, please download the PDF or contact us.

• Food Testing/Analysis/Measuring Equipment
• Contract Analysis
• Other safety and hygiene products

This catalog introduces the "Microbial Identification Tests for Hygiene Management" provided by Technosulga Lab Co., Ltd., a company specializing in microbial identification testing services. It features a variety of tests, including the "Microbial Confirmation Test," which allows for the confirmation of product deterioration and appearance abnormalities under a microscope, and "Microbial Isolation," which accommodates a wide range of samples such as food, feed, water, soil, and leather products. Additionally, it includes a basic flow for requesting tests and guidance on sample submission. [Contents] ■ Information and Common Guide ■ Microbial Tests for Hygiene Management ■ Other Microbial Tests, Products, and Services *For more details, please download the PDF or contact us.

• Food Testing/Analysis/Measuring Equipment
• Other safety and hygiene products
• Other contract services

【Features】 - Detailed identification tests for yeast - Set menu with comprehensive discussion of genetic analysis results and morphological observations - Content designed for data use during academic papers and patent deposits - Reports include microscopic photographs of colony morphology and cell morphology observations - Identification results of the assigned species and closely related species, along with biosafety levels 【Overview】 Yeast Premium is a menu that combines "DNA sequencing analysis" with trait tests such as "morphological observations" and "physiological and biochemical property tests." Through comprehensive discussions, it enables more detailed estimation of assigned species and closely related species, as well as taxonomic characterization. This service is effective for customers who require more detailed information about research, product development, and microbial strains, such as for conference presentations and patent applications. This test is a service for detailed examination and analysis of your existing strains. For rapid identification, please check our "Rapid Analysis."

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【Features】 - Contracted detailed microbial testing including genetic analysis, morphological observation, and physiological/biochemical property tests. - Selection of physiological/biochemical property test items effective for taxonomic characterization based on genetic analysis results. - Reporting with biosafety levels for assigned species and closely related species. 【Overview】 This is a menu that combines "16S rDNA-Full (with discussion)," which is a full-length analysis of 16S rDNA, with property tests such as "morphological observation" and "physiological/biochemical property tests." Through comprehensive discussion, it is expected to provide a more detailed estimation of assigned species and closely related species compared to tests that only analyze DNA sequences. This service is effective for customers who require more detailed information about microbial strains for academic presentations or patent applications, enabling taxonomic characterization of the relevant microbial strains. 【Analysis Flow】 1. Cultivation and purity confirmation 2. DNA extraction 3. PCR amplification 4. Sequencing 5. Sequence determination 6. Homology search 7. Phylogenetic analysis 8. Literature review 9. Bacterial first-stage testing 10. Bacterial second-stage testing 11. Comprehensive discussion This test is a service for detailed examination and analysis of your bacterial strains. For rapid identification, please check our "Rapid Analysis" service.

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**Overview** For analysis methods, you can choose from two types of analysis software (Metagenome@KIN and QIIME2). 〇 Analysis using Metagenome@KIN - The 16S rDNA of bacteria and archaea is analyzed using two types of databases: RDP (Ribosomal Database Project) and a microbial identification database. - The microbial identification database allows you to change the BLAST cut-off criteria from a homology rate of 97% to a specified value. - For three or more samples, cluster analysis and principal component analysis will be conducted. 〇 Analysis using QIIME2 - The Amplicon Sequence Variant (ASV) method allows for the identification of closely related species with high homology rates. - Secondary analyses required for academic papers (such as diversity analysis and statistical analysis functions) are well-equipped. **Acceptable Data** Sequence data from next-generation sequencers (fastq files) can be analyzed. Sequence data obtained from various samples such as gut microbiota, skin microbiota, oral microbiota, soil, and activated sludge can be analyzed. Additionally, data analysis using the statistical analysis software R and predictive metagenomic analysis using PICRUSt2 are also accepted.

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The skin microbiome is gaining attention in fields such as skin diseases, cosmetic development, and anti-aging. This kit is designed for the collection and preservation of skin resident bacteria, allowing for easy sampling. Utilizing the specimen preservation techniques developed for gut microbiome analysis, it enables the maintenance of the microbiome even at room temperature. It can be used for microbiome analysis through amplicon sequencing. In this kit, skin resident bacteria are collected by rotating a cotton swab soaked in saline on the skin, and the swab is then immersed in a preservation solution to minimize changes in the microbiome. Unlike adhesive tape, it causes less damage to the stratum corneum during collection. If you send the kit with the collected samples to us, you can also utilize our contracted analysis for microbiome analysis (amplicon sequencing). Please feel free to contact us for information on usage and other inquiries.

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• Other laboratory equipment and containers
• Contract Analysis

【Overview】 ○ This is a contract service for extracting DNA from feces, saliva, skin swabs, etc., and delivering the extracted DNA. Even if you do not have the know-how for DNA extraction in-house, you can obtain extracted DNA through outsourcing. ○ It can be used as template DNA for PCR. It can be utilized for microbial community analysis of gut flora, oral cavity, skin, and more. 【Acceptable Samples】 〇 Category A Feces and intestinal contents from humans, mice, rats, pigs, cattle, horses, chickens, dogs, cats, fish, insects, etc. 〇 Category B Human saliva, plaque, skin swabs, vaginal swabs 【Delivery Contents】 - DNA extract (0.5 mL tube) - DNA concentration measurement results (Excel file) We will deliver at least 70μL of DNA dissolved in sterilized water or TE buffer. We will report the DNA concentration measurement results obtained by absorbance. We also accept DNA extraction from various samples such as soil and activated sludge. Please feel free to contact us if you have any questions.

• Other services
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【Overview】 This is a contract testing analysis service that analyzes the physicochemical components in feces. The intestinal environment is gaining attention in relation to research and development in fields such as medicine, anti-aging, and probiotics. To clarify the relationship between the intestinal environment and host health, it is important to deeply understand the relationship between the gut microbiota and its metabolites. In addition to gut microbiota analysis, we offer contract analysis of intestinal metabolites such as organic acids (short-chain fatty acids) and physicochemical analysis of the intestinal environment, including IgA production and bile acids. 【Analysis Items】 - Analysis of organic acids in feces (short-chain fatty acids) - Analysis of bile acids in feces - Analysis of putrefactive products in feces - Analysis of ammonia in feces - Measurement of fecal pH - Measurement of fecal moisture - Analysis of IgA in feces (human) 【Sample Acceptance Records】 Feces and intestinal contents from humans, mice, rats, pigs, cows, horses, chickens, dogs, cats, as well as fish and insects. 【Sample Submission Method】 - Please send frozen feces. - For organic acids (short-chain fatty acids), the sample collection kit "MetaboloKeeper" is convenient. Samples can be stored at room temperature. Please feel free to contact us for details on how to request services and analysis.

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**Features** - Contracted service for mold morphology observation - High-precision mold morphology observation by experienced technicians - Reports include microscope images of colony morphology and cellular morphology observation results - Conducting as an additional test for genetic analysis allows for comparative discussion with related and affiliated species **Overview** - By observing mold morphology, it is possible to investigate taxonomically important features. - In mold morphology observation, basic forms such as colony morphology and coloration are examined. The results, including microscope images, are delivered as a report. - In the first-stage mold test, the affiliated taxon at the genus level is identified through macroscopic and microscopic morphology observation. The classification system generally adheres to "Index fungorum" or "MycoBank." - In the second-stage mold test, a more detailed morphology observation is conducted as an additional test to the first-stage mold test or genetic analysis, comparing with literature data to comprehensively identify the affiliated taxon at the species level. **Additional Tests** By conducting DNA sequencing analysis as an additional test, more detailed and multilayered identification is possible.

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- I want to estimate whether it belongs to B. cereus or B. wiedmannii (both BSL2) or to other species → Multiplex PCR test / Multiplex PCR Rapid test* - I want to confirm whether it produces diarrhea toxin or not → Diarrhea toxin detection test* *Items marked with an asterisk are only conducted for strains where B. anthracis has been ruled out. The Bacillus cereus group is difficult to distinguish among 18 species based on 16S rDNA sequence analysis. The Bacillus cereus group includes species that are extremely important from the perspective of biosafety, infection prevention, and medical laws concerning patients with infectious diseases, such as B. anthracis (anthrax) and B. cereus (cereus). Please use this when strains suspected to belong to the Bacillus cereus group are detected.

• Contract Inspection
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- Free trial currently available (1 month) - 【Features】 - Significantly improves the efficiency of microbial identification using DNA sequencing - Equipped with an automatic analysis function that does not require specialized knowledge (including molecular phylogenetic analysis) - Utilizes a "microbial identification database" composed solely of effective sequence data for microbial identification, co-developed with the National Institute of Genetics, Life Information and DDBJ Research Center - Provides two types of databases for bacteria and fungi (molds and yeasts). Regular updates accommodate the registration of new species and changes in scientific names due to transfers - Includes a user database function that allows for comparative analysis with previously analyzed data - Displays biosafety levels for easy safety assessment 【Overview】 In DNA sequence analysis, the use of public databases is common, but due to the vast amount of sequence data accumulated, selecting effective data for microbial identification requires significant effort and time. Additionally, there are many cases where identification is difficult without specialized knowledge in microbial taxonomy. With "ENKI," you can easily perform similarity searches and phylogenetic analyses. The analysis menu buttons are arranged according to the analysis steps, making it easy for customers without specialized knowledge to operate. Furthermore, these tasks can also be performed automatically.

• Analytical Equipment and Devices
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This is a contract service for producing ampoules that can be stored long-term by sending microbial strains. 【Low Cost and Space Saving】 The storage ampoules are suitable for long-term preservation of microbial strains at low cost and with minimal space requirements. The ampoules are small glass tubes measuring 8 cm in length and 9 mm in diameter, taking up little storage space. They can be stored in a refrigerator (4°C), eliminating the need for freezing equipment like deep freezers. Since subculturing is not required, costs associated with media and maintenance work can be reduced. 【Stable Long-term Preservation】 We produce storage ampoules using the L-drying method, which excels in preservation and cost. The storage ampoules made by the L-drying method are adopted by deposit institutions for patented microbial strains as a method that meets the conditions for maintaining patented microbial strains during the 20-year patent period. 【Compliance with NPMD Standards】 The storage ampoules produced by our company conform to the standard sizes of the National Institute of Technology and Evaluation (NPMD), which is the deposit site for patented microbial strains in Japan. 【Backup for Useful Strain Loss】 We also offer storage services for ampoules. By distributing valuable microorganisms across multiple facilities and regions, we reduce the risk of loss of microbial strains due to disasters and other events.

• Other contract services

【Please use this at such times】 We will check at the microscopic level whether there are microorganisms in the collected samples, such as discoloration of building materials, foreign substances in factory water, slime in pipes, discoloration of food, and foreign substances in raw materials. If microorganisms are present, we will determine and report the category of the microorganisms, such as bacteria, mold, or yeast, as a contracted service. The received report can be used for internal investigation reports or reports to third parties. 【Is this foreign substance a microorganism?】 When foreign substances, strange odors, discoloration, swelling, or other abnormalities occur in products or manufacturing sites, you may wonder, "Something is wrong, but I don't know if the cause is microorganisms (bacteria, mold, yeast)." Without specialized knowledge or equipment, it becomes very difficult to determine the presence of microorganisms. 【As the first step in confirming foreign substances】 Please use this as the first step in confirming foreign substances, such as "I don't need identification, but I want to first check if there are microorganisms in the foreign substance." If you wish to identify the type of microorganism, we will propose additional tests for isolation and identification according to your purpose and budget.

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【Overview】 〇 This is a contract analysis service that quantitatively measures the DNA of bacteria, archaea, and fungi contained in samples. It can be used to compare microbial quantities between samples. 〇 The quantity of DNA that is exponentially amplified in real-time PCR for specific taxonomic groups present in the sample is monitored in real-time. 〇 For certain types of microorganisms, it is also possible to convert copy numbers to cell counts. 【Features】 〇 Quantification of microorganisms (bacteria, fungi, yeast, archaea) is possible. 〇 The absolute amount (copy number) of target microbial template DNA is estimated using our proprietary primer set. 〇 We possess many primers, including original primers for intestinal bacteria, oral bacteria, skin resident bacteria, and microorganisms in environmental and food samples. 〇 Accurate estimation is possible even from minute samples. 【Examples of Acceptable Samples】 〇 Animal feces and intestinal contents from mice, rats, pigs, cattle, horses, pets, etc. 〇 Saliva, plaque, skin swabs, vaginal swabs. 【Target Bacterial Groups】 Please refer to the related links. For other inquiries regarding testing methods or acceptable samples, please feel free to contact us.

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【Features】 - Contract analysis service to quantify the amount (copy number) of target microbial template DNA using our company's proprietary primer set. - Possession of many original primers capable of detecting environmental and food-related microorganisms. - Accurate quantification is possible even from trace samples. 【Overview】 Quantification of genes from specific taxonomic groups present in the sample. The amount of DNA, which is exponentially amplified, is monitored in real-time using a real-time PCR device. By comparing the DNA copy numbers of total bacteria and total archaea, it is also possible to estimate the proportion of target microbial groups in the sample. 【Acceptable Sample Examples】 Food samples such as fermented foods, activated sludge samples, septic tank samples, soil and soil-related samples such as volcanic ash, compost, fermentation samples such as culture media, water samples from seawater and lakes, contents of fermentation tanks, DNA extracts where PCR amplification has been confirmed, etc. 【Target Microbial Groups】 Many microbial groups can be detected and quantified. Please refer to the related links. Feel free to contact us regarding testing methods and detectable microbial groups. You can also check the details from the related links.

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Identifies strain-level differences that cannot be distinguished at the species level. It is a contract service that rapidly and cost-effectively identifies microbial strain differences. For example, it is useful for distinguishing between multiple strains of lactic acid bacteria that are the same species and are held as beneficial strains. It can also be used to identify beneficial strains of microorganisms contained in health foods such as probiotics. For instance, if you have many strains of the same type of microorganisms, such as lactic acid bacteria or yeast, it can be utilized for rapid and cost-effective grouping of those microbial strains. It can be conducted more quickly and cheaply compared to traditional identification methods such as PFGE analysis, MLST analysis, and whole genome analysis. It identifies differences in microbial strains using the latest IR Biotyper. [About IR Biotyper] A new identification method for microbial strains has been proposed by Bruker using the IR Biotyper. The IR Biotyper is a system that acquires Fourier Transform Infrared (FT-IR) spectral data of carbohydrates and polysaccharides from different microbial strains cultured under the same conditions, and performs comparative analysis of the spectral data between microbial strains to achieve strain-level identification.

• Contract Analysis

- A service that conducts analysis of the microbiome (bacterial, archaeal, and fungal communities) in samples. - Supports various samples such as soil, water, biofilms, compost, contents of fermentation tanks, activated sludge, contents of septic tanks, fermented foods, probiotics, and spoiled foods. - Amplicon sequencing analysis using next-generation sequencers (MiSeq, Illumina). - We can handle the extraction of DNA and RNA, so you can send the samples directly to us when making a request. - The delivered data can be widely used, from R&D to academic papers. - Service highlights: - Excellent cost performance, starting from 22,000 yen per sample. - Comprehensive analysis of bacterial/archaeal microbiomes. - Analysis of sequences approximately 430 bp long for bacteria and approximately 385 bp long (paired-end) for archaea. - Analysis of fungal communities using the ITS region. - Acquisition of about 10,000 reads, allowing detection of low-abundance taxa. - Support for RNA analysis as well. - We also accept requests for analysis only from already acquired sequence data. Please feel free to contact us regarding analysis methods and other inquiries.

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This is a technical training program to learn basic techniques for handling microbial cultures, as well as fundamental techniques for microbial identification through DNA sequence analysis, including DNA extraction, PCR, and DNA sequencing analysis. In addition to seminar-style lectures, practical sessions are included to deepen understanding of microorganisms. Due to the small class size, participants can ask questions about anything they do not understand or are interested in at that moment. The instructors are researchers with extensive experience in handling microorganisms who are responsible for our contract testing. For the latest information on scheduled dates and participant recruitment, please refer to our company website. The application deadline is two weeks before the event date. Participants will receive a "Certificate of Completion," making it ideal for new employee training and staff education. If two or more participants from the same organization attend the same seminar, the fee for the additional participants will be discounted by 20%. Feedback from participants in post-training surveys has been positive, with comments such as "It was easy to understand as we could conduct experiments simultaneously with the instructor" and "All my questions were answered thoroughly, resolving my doubts."

• Technical Seminar

【Overview】 This is a contracted testing and analysis service that observes iron bacteria and reports their types. In this test, the presence of iron bacteria is observed using an optical microscope, primarily focusing on water samples from the environment (including samples with brownish precipitates), and results are reported with images and identification results. Observations are conducted using Gram staining and unstained methods (such as differential interference observation), referencing sewage testing methods (2012 edition) and ASTM D932-20 (2020). Microscopic observation of iron bacteria in accordance with the international standard ASTM International's "Standard Test Method for Iron Bacteria in Water and Water-Formed Deposits (D932-20 (2020))" is also available. 【Iron Bacteria】 A group of bacteria that have the ability to oxidize dissolved iron and dissolved manganese in water, depositing them both inside and outside their cells, are referred to as iron bacteria. Filamentous microorganisms, including iron bacteria (primarily filamentous bacteria), are detected in places where groundwater containing dissolved iron emerges, and they can cause issues such as microbial corrosion, bulking of activated sludge, and clogging of biofilms, which are problematic for the maintenance and management of biological treatment facilities for wastewater and similar applications.

• Contract Inspection

This is a commissioned service to investigate information regarding the properties of microorganisms from academic papers, journals, specialized books, and textbooks related to the specified microbial species. To examine characteristics such as scientific name, Gram staining properties, isolation source, optimal growth temperature, reports of isolation sources, antibiotic resistance, and pathogenicity of the species, it is necessary to research many information sources like papers and specialized books, which requires time and effort. By outsourcing this investigation, efficiency in internal operations is expected. The subjects of the investigation are microorganisms (bacteria, fungi, yeast). You can use this service if the species name of the microorganism is specified. Please provide the species name (scientific name) when making your request (the genus name alone is insufficient for investigation). Specialized researchers will conduct the investigation and report reliable information with clear citations. This service can be utilized to understand the general characteristics of microbial strains identified by methods such as DNA analysis.

• Contract Inspection

If you send us the samples, we offer contract testing and analysis services to isolate and separate bacteria, molds, and yeasts contained in the samples. Various types of samples can be accepted, including fermented foods, spoiled foods, biofilms, environmental samples such as feed, water, and soil, as well as contaminated items (daily necessities, leather products, etc.). You can also use our services for screening beneficial microorganisms such as lactic acid bacteria and yeasts. For the isolation of microorganisms present in special environments (such as sulfate-reducing bacteria and iron-oxidizing bacteria), please contact us separately. You can specify the culture conditions (aerobic, anaerobic, high temperature, low temperature, etc.) and target microorganisms such as spore-forming bacteria, E. coli, lactic acid bacteria, acetic acid bacteria, and actinomycetes to isolate the desired strains. The isolated and separated microbial colonies can be preserved as pure cultures, and identification tests can be conducted as an additional service (additional fees apply). We also have a variety of additional tests available for identifying the types of microbial strains after isolation. Please feel free to contact us. We distinguish colonies using a compound microscope or an optical microscope.

• Contract Inspection

This is an amplicon sequencing analysis using next-generation sequencers (MiSeq, Illumina). It is a service that offers microbiome analysis of various samples, including intestinal microbiota (gut flora) analysis, skin resident microbiota analysis, and oral microbiota analysis. We handle DNA and RNA extraction, PCR, sequencing, and analysis all in one package. Please send us samples such as feces, intestinal contents, or saliva when making a request. The delivered data can also be used for academic papers. By using our collection kit, it is possible to store samples at room temperature. The results obtained from microbiota analysis represent the relative abundance of specific microbial groups, not absolute quantities. By combining with real-time PCR, the absolute quantity of microorganisms can also be quantified in terms of DNA amount. Service highlights: - Excellent cost performance - Comprehensive analysis of bacterial/archaeal microbiota - Analysis of sequences approximately 430 bp long for bacteria and approximately 385 bp long (paired-end) for archaea - Analysis of fungal communities using the ITS region - Acquisition of about 10,000 reads, allowing detection of low-abundance taxa - Support for RNA analysis - We also accommodate requests for analysis only from already obtained sequence data - Options for α and β diversity analysis, principal component analysis, cluster analysis, etc.

• Contract Inspection

- Rapidly identifies the names and types (scientific names) of microorganisms. - Recommended for customers in pharmaceutical and food & beverage manufacturing who are responsible for hygiene management, testing, and inspection, particularly those requiring rapid and accurate identification. - The Rapid analysis using DNA sequencing compares with the International Nucleotide Sequence Database (INSD: DDBJ/ENA/GenBank) and the microbial identification DNA database & phylogenetic analysis system "ENKI" to estimate the assigned species and closely related species. The bacterial database covers over 80% of known reference strains. - The report summarizes the analysis results, the assigned species or closely related species names, biosafety levels, and other information. - Comprehensive service from DNA extraction, PCR, sequencing, to identification. The protocol is based on the Japanese Pharmacopoeia, ensuring that pharmaceutical manufacturers can use it with confidence. 【Features】 - Reliable identification results based on academic evidence and experience. - High repeatability due to the rapid and reliable identification results. - Rapid reporting within 2 to 4 business days. - DNA sequencing analysis: Bacterial 16S rDNA region / Fungal ITS region / Yeast D1/D2 region. - Understanding phylogenetic relationships with pathogenic bacteria and closely related species.

• Contract Inspection

**Service Features** - Rapid identification contract testing, analysis, and inspection services. - Simply send agar plates with microbial colonies. - For bacteria and yeast, identification results are delivered on the same day for samples arriving by noon. For molds, results are delivered in as little as 4 business days. - Identification results are at the species level. The report includes the species name and its biosafety level. - MALDI microbial identification testing is faster and more cost-effective compared to DNA sequencing analysis. - It can identify with higher accuracy than traditional morphological observation or simple identification kits, identifying a greater number of species. - Outsourcing microbial identification reduces costs for equipment maintenance and reagent purchases, lowering the expenses of the testing department. - Ideal for rapid and inexpensive identification for hygiene management purposes. - Effective for cost-effective identification of microbial strains detected in large quantities, such as in bio-burden studies. **Report Contents** - Candidate species (identification results) inferred from the database matching results and confidence values (scores). - Biosafety level (BSL) of the candidate species. - Reference data: Results report showing the top 10 database-registered microbial strains with high homology in matching results. Please feel free to contact us regarding testing methods or the content of the results.

• Contract measurement

All industries are paying attention to microbiological testing! For example, pharmaceuticals, food, industrial products, energy, waste treatment, and environmental purification. Our company addresses the safety of food, environment, and products with specialized staff in microbial taxonomy. We also host seminars on microbial identification techniques. ■ Microbial identification testing ■ Microbial community structure analysis ■ Microbial taxonomy research

• Contract Inspection