ファスマック List of Products and Services

For those considering next-generation sequencing analysis, we offer a downloadable document that introduces numerous specific analysis examples. Additionally, you can view actual analysis data and related services through links within this document, making it easier to visualize the content. ★You can download the document using the "PDF Download" button below. 〈Contents (excerpt)〉 ■What are the benefits of next-generation sequencing analysis? ■What types of samples can be analyzed? ■What kind of data can be obtained? We provide various plans tailored to our customers' objectives and strive to offer proposals based on your requests. If you are interested, please feel free to contact us.

• Contract Analysis

The "Fruit Detection Primer" is a reagent used to detect DNA in food by PCR method for "apple," "peach," and "kiwifruit," which are designated as "specific raw materials." Please use it for quality control of various foods. Additionally, our company conducts inspections of specific raw materials in food in accordance with the testing methods specified by the Consumer Affairs Agency. We offer a wide range of testing menus, from simple tests to confirmation tests. Our testing system has been consistently evaluated as having high reliability through international testing system evaluation tests. 【Features】 ■ Detection of DNA in food for "apple," "peach," and "kiwifruit" by PCR method ■ Usable for quality control of various foods ■ Free samples for trial are available *For more details, please refer to the PDF document or feel free to contact us.

• Testing Equipment and Devices
• Contract Inspection

This product is a primer set for identifying foreign substances derived from insects. Unlike the current mainstream identification based on morphological characteristics, this method uses DNA sequences as indicators, allowing for accurate insect species identification without the need for skilled techniques. Please use it as one of your quality control tools. In recent years, consumer interest in food safety and security has increased, and particularly, foreign substances mixed into food have become a major factor causing consumers to have a negative image of food. To ensure reliability and improve quality for our customers, we provide higher quality foreign substance identification testing. 【Features】 - Primers are designed to amplify regions that reflect differences in insect species within the cytochrome c oxidase subunit I gene and the 16S ribosomal RNA gene. - Since it uses short DNA sequences as indicators, it can also identify insect foreign substances that have undergone heat processing. - By combining three primer sets, more accurate determinations can be made. - We offer free samples for testing. *For more details, please refer to the PDF document or feel free to contact us.

• Testing Equipment and Devices
• Contract Inspection

This product is a primer set for identifying foreign substances derived from plants. Unlike the current mainstream identification based on morphological characteristics, this method uses DNA sequences for a more objective identification approach. Please use it as one of your quality control tools. In recent years, consumer interest in food safety and security has increased, and particularly, foreign substances mixed into food are a major factor contributing to negative perceptions among consumers. To ensure reliability and improve quality for our customers, we provide higher quality foreign substance identification testing. 【Features】 - Primers are designed to amplify specific regions (ITS) for each plant species. - Amplifies a wide range of plants while not amplifying molds or yeasts. - The PCR amplification products are relatively short at about 350–400 bp, making them easier to amplify even if the DNA is damaged due to heating or other factors. - Free samples for testing are available. *For more details, please refer to the PDF document or feel free to contact us.

• Testing Equipment and Devices
• Contract Inspection

"PNA (Peptide Nucleic Acid)" is a non-natural compound with a DNA-like structure. Its structure differs from that of DNA and RNA, as it forms a backbone with peptide bonds instead of phosphate bonds, which is why it is called peptide nucleic acid. It has excellent properties as an antisense and probe. The repulsion caused by the negative charge from phosphate groups found in conventional DNA is eliminated, allowing for stronger hybridization and improved complementary strand recognition. Additionally, "PNA synthesis" is entrusted to the South Korean company Panagene. For a formal estimate, please send us information such as the sequence and desired yield, and we will confirm the feasibility of synthesis with Panagene. *For more details, please refer to the related links or feel free to contact us.*

• Other contract services

"Fragment analysis" involves customers sending PCR products amplified using fluorescent primers, which are then assessed for fragment length through capillary electrophoresis by a sequencer. Our company conducts fragment analysis using a capillary sequencer to assist with DNA variety testing and technological development. We also aim to provide proposals tailored to our customers' needs, so please feel free to contact us. 【Features of DNA Sequencing Service】 - Analysis conducted domestically allows for detailed and prompt responses. - All data is visually checked by specialized staff. - The sequencer has undergone official maintenance by the manufacturer. - Re-electrophoresis is performed on samples expected to show improved data. *For more details, please refer to the PDF document or feel free to contact us.*

• Contract Analysis

The "Cas9 protein" is commonly used in genome editing via CRISPR/Cas and is a double-strand cutting type. The storage temperature is below -20°C. We offer a 100μg version "GE-005-S" and a 100μg x3 version "GE-005-L". We provide the "Genome Craft series," a suitable genome editing tool that achieves highly efficient knock-in/knock-out in CRISPR/Cas-based genome editing technology. 【Features】 ■ Commonly used double-strand cutting type in genome editing via CRISPR/Cas ■ Storage temperature: below -20°C (long-term storage recommended at -80°C) ■ Storage buffer: 10mM Tris-HCl (pH 7.5), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol ■ Protein source: Recombinant E. coli *For more details, please refer to the PDF document or feel free to contact us.

• Software (middle, driver, security, etc.)

Here are examples of the use of the genome editing tool "Genome Craft." We mixed Cas9 protein with sgRNA or crRNA + tracrRNA and substrate DNA fragments, and conducted a DNA cleavage experiment. After incubating at 37°C for one hour, we performed agarose gel electrophoresis. The shift of the bands confirmed that both sgRNA and crRNA + tracrRNA exhibited good DNA cleavage activity. In addition, there are other examples of use such as "In vivo Genome Editing," "double knockout," and "gene knock-in (mouse)." [Examples of Use] ■ In vitro digestion assay ■ In vivo Genome Editing ■ Double knockout (zebrafish) ■ Gene knock-in (mouse) *For more details, please refer to the PDF document or feel free to contact us.

• Software (middle, driver, security, etc.)

<Strengths and Features of Fasmac Co., Ltd.> ■ World-class inspection and manufacturing technology ISO17025 certification, ISTA certification, ISO9001 certification ■ Commitment to research and development Standard analysis methods for GM foods, GM seed screening technology, and many others ■ Robust infrastructure and sales channels Various DNA analysis devices, numerous skilled technicians Established sales network to various research institutions <Research and Development Achievements (Partial)> ■ Improvement of genome editing technology CRISPR/Cas method Joint research with Tokyo Medical and Dental University, Hiroshima University, and Keio University ■ Random integration analysis Joint research with the National Institute of Infectious Diseases ■ Molecular quantity guaranteed DNA standard materials Joint research with Ricoh Company, Ltd. ■ Development of a skin resident bacteria detection kit using DNA chromatography methods Joint research with Menard Co., Ltd. *If you have any concerns, please feel free to contact us. We aim to add new value to existing platforms and hope to collaborate with all of you.

• Contract Analysis
• Contract Inspection
• others

【Features】 ■ Achieves output of up to 640G of sequence data per run ■ Data quality comparable to Illumina's sequencers ■ Realizes low cost, increased data volume, and multi-sample analysis Comparison data between Illumina's Next-seq and DNB-SEQ (RNA-seq) is available on our website.

• Contract Analysis

We would like to introduce a case study of our random integration analysis service using the RAIS method. We performed the RAIS method on T-DNA taglines inserted into Arabidopsis thaliana using the Agrobacterium method, as well as on mouse cultured cells with retrovirus vectors inserted. We confirmed the insertion site sequences using Sanger sequencing and mapped the decoded sequences obtained from next-generation sequencing analysis to the genome sequence. As a result, it was confirmed that this method is effective for determining insertion sites in animal cultured cells as well as in plant cells. 【Random Integration Analysis Service using the RAIS Method】 ■ Sanger Sequencing Plan ■ Next-Generation Sequencing (NGS) Plan * For more details, please refer to the PDF document or feel free to contact us. * For case studies, please refer to the URL below.

• Contract Analysis
• Contract Analysis
• Analysis Services

This document includes the purpose of working on the JFS standards and the process for obtaining conformity certification. It provides detailed information using diagrams and illustrations about the implementation schedule for the HACCP system, the types of JFS standards, and the process for obtaining conformity certification for JFS-A/B standards. Please feel free to utilize this information in your efforts related to HACCP. 【Contents (partial)】 ■ About HACCP - Implementation schedule for HACCP system - Image of HACCP - PDCA cycle for hygiene management ■ About JFS standards - Utilization of private certification - Types of JFS standards - Number of organizations for JFS standards conformity certification and accreditation *For more details, please refer to the PDF document or feel free to contact us.

• others

We offer DNA/RNA synthesis services. We support a wide range of nucleic acid synthesis, including cross-linked artificial nucleic acids (LNA), 2’F (fluoro) RNA, 2’MOE (2’-O-methoxyethyl) RNA, and 2’-OMe RNA. These can be utilized for applications such as antisense research and partial incorporation into dual-labeled probes. We can also accommodate insertions into DNA and RNA, as well as combinations with various modifications, so please feel free to consult us. 【Overview】 ■ Nucleic Acid Derivatives ・Artificial Nucleic Acids (LNA) ・2’F (Fluoro) RNA ・2’MOE (2’-O-methoxyethyl) RNA ・2’-OMe RNA ■ Nuclease-Resistant Modifications ・S-Oligo (Phosphorothioate) ・Inverted dT, etc. *For more details, please refer to the PDF document or feel free to contact us.

• others

The classification and identification of bacteria and fungi have traditionally been conducted through methods such as morphological observation under a microscope and isolation culture. However, it is said that identification through culture methods relies heavily on experience. In this context, methods utilizing genetic analysis technology are gaining attention. They offer the advantage of obtaining analysis results with high discrimination ability and reproducibility. Our company has launched a microbial species identification testing service using genetic analysis technology. We hope it will be useful for your quality control. 【Features】 ■ High discrimination ability and reproducibility through sequence analysis ■ High reliability due to a high-precision database * Uses Applied Biosystems' "MicroSeq ID System Database" ■ Supports both bacteria and fungi (molds, yeasts) * For more details, please refer to the PDF materials or feel free to contact us.

• others

In recent years, consumer interest in food safety and security has been increasing, and particularly, foreign substances mixed into food have become a major factor in consumers developing a negative image of food. To ensure reliability and improve quality for our customers, our company offers higher quality foreign substance identification testing. 【Testing Details】 ■ Screening Tests ■ Plant Foreign Substance DNA Testing ■ Insect Foreign Substance DNA Testing *For more details, please refer to the PDF document or feel free to contact us.

• others

Our company conducts inspections of specific ingredients in food in accordance with the inspection methods specified by the Consumer Affairs Agency notification law. We offer a wide range of testing menus, from simple tests to confirmation tests. Our testing system has been consistently evaluated as high quality through international testing system evaluation tests. Additionally, in response to customer requests, we also provide our own unique optional tests that are not listed in the notification law. Please feel free to consult with us. 【Features】 ■ Extensive testing experience and high technical capabilities ■ A wide range of testing menus from simple tests to confirmation tests ■ Flexible services including proposals for testing content and preparation of English reports ■ Support for many items corresponding to specific ingredients ■ ISO/IEC 17025 laboratory accreditation obtained *For more details, please refer to the PDF materials or feel free to contact us.

• others

Our "Seed Inspection" is conducted in accordance with the inspection methods established by international organizations. In pathological testing, we adopt the inspection methods published by ISTA, ISHI, and IPPC. We have a lineup focused on plant pathogenic microorganisms designated as "quarantine harmful plants and animals" under the Plant Protection Law. Please use it as an external inspection and external certification in conjunction with your company's quality inspection. 【Features】 ■ Adoption of inspection methods published by ISTA, ISHI, and IPPC ■ Lineup focused on plant pathogenic microorganisms ■ Conducted in accordance with international organizations' inspection methods ■ Available for use as external inspection and external certification alongside quality inspection ■ Issuance of ISTA International Seed Testing Certificates (Blue Certificates) is possible *For more details, please refer to the PDF materials or feel free to contact us.

• others

This product is a kit for testing pathogens (TYLCV, Aac, MYSV) using antibodies through a gold colloid immunochromatography method, designed to examine suspected infected areas. The Aac detection kit also allows testing from cultured strains (colonies). A sample of the test specimen and the extracted liquid are dropped onto the test strip, with the required time for results being 15 to 30 minutes for TYLCV and MYSV, and 10 to 15 minutes for Aac. ★ Due to popular demand, the campaign period has been extended! Until the end of March 2022, 10,000 yen per kit. 【Product Lineup】 ■ Tomato yellow leaf curl virus ■ Bacterial fruit blotch of cucurbit vegetables ■ Melon yellowing virus * For more details, please refer to the PDF document or feel free to contact us.

• others

"Nano Trap Easy" is a food allergen testing kit specifically designed for wipe solutions and rinse water. It detects individual allergens with high sensitivity. Switching from or combining with protein residue testing increases the reliability of cleaning verification. Additionally, since it does not use extraction or dilution solutions, it can be used for testing in manufacturing environments. 【Features】 ■ No pre-treatment required, easy and quick ■ No reagents used ■ Easy to handle ■ Detects allergenic proteins from 0.5 ppm ■ Individually packaged & contains 10 units *For more details, please refer to the PDF document or feel free to contact us.

• others

【Features】 ■ Inspections can be conducted regardless of whether processing such as heating or pressurization has occurred. ■ Applicable to a wide range of samples (products, semi-finished products, raw materials, wipe samples, etc.). ■ Fewer false positive reactions lead to more accurate test results. ■ Testing is possible for samples with high viscosity. ■ The contamination of specific raw materials can be quickly determined within 15 minutes after dropping the test solution. (30 to 60 minutes from preparation to determination) ■ Results can be visually assessed without the need for special equipment.

• others

The "Morinaga FASPEK Eliza II" is an improved testing kit that meets the standards set forth in the Food Safety Notification No. 286 dated September 10, 2010, regarding "Testing Methods for Foods Containing Allergenic Substances." Compared to conventional kits, it does not use toxic substances in the extraction solution, thereby reducing reagent management and environmental impact. Additionally, since the simple testing kit "Nano Trap II R" shares the same extraction solution, it is possible to quantify the extraction solution of samples that tested positive with Nano Trap IIR directly with this product, significantly shortening the time required. 【Features】 ■ Efficiently extracts proteins ■ Shows excellent reproducibility down to around 1 ppm ■ Short reaction time of 1 hour and 50 minutes ■ Quantifiable from 0.31 ppm as specific raw material protein ■ Adopted in ELISA kits compliant with notifications from various companies *For more details, please refer to the PDF materials or feel free to contact us.

• others

We provide "Audit and Consulting (Support for the Institutionalization of HACCP)." Starting with a diagnosis of the current status regarding HACCP compliance, we offer support tailored to our customers' needs and situations, including assistance with obtaining JFS standards and JFS standard audits. "If you don't know how to respond to the institutionalization of HACCP" "I am worried whether our current facilities and equipment can comply with HACCP institutionalization" If you have such concerns, please contact us. 【Support for HACCP Institutionalization】 ■ Assistance with HACCP institutionalization - Diagnosis of the current status regarding HACCP compliance - Employee training on HACCP - Assistance in creating HACCP plans, etc. ■ Support for obtaining JFS standards ■ JFS standard audits ■ Contract testing related to food hygiene ■ Sale of testing kits *For more details, please refer to the PDF document or feel free to contact us.

• Audit/Accounting

This product is a 2× premix type PCR reagent. It contains Taq DNA Polymerase, dNTPs, Mg2+, etc., making it possible to perform PCR by simply adding template DNA and primer pairs. The Taq DNA Polymerase included in this product is designed for hot start PCR, preventing the amplification of products caused by misannealed primers during the temperature ramp-up process at the start of PCR. 【Features】 - High specificity PCR is possible due to optimized buffer composition. - As a premix type, it eliminates the need for complicated reagent preparation. - It has TdT activity, allowing PCR products to be used for TA cloning. *For more details, please refer to the PDF document or feel free to contact us.

• others

This product is a reagent for easily extracting DNA from various samples such as blood, animal tissues, plants, bacteria, fungi, and food. It allows for DNA extraction in a short operation time of approximately 15 minutes. The obtained DNA solution can be used as a template for PCR and real-time PCR, among other applications. 【Features】 ■ Extraction possible in one tube ■ The process involves only heat treatment and centrifugation ■ Operation time is about 15 minutes ■ Extracted DNA can be used as a template for genetic testing *For more details, please refer to the PDF document or feel free to contact us.

• others

This product is a 2× premix type reagent for real-time PCR. It contains heat-stable DNA polymerase, Mg2+, Passive Reference Dye, and optimized buffers necessary for real-time PCR (fluorescent probe method), allowing it to be used simply by adding template DNA, primer pairs, and probes. The heat-stable DNA polymerase included in this product is prepared for hot start PCR and is activated by a heat treatment of 95°C for 10 minutes. 【Features】 ■ Enables highly specific real-time PCR ■ No complicated reagent preparation required ■ Compatible with various real-time PCR devices (plate type) ■ Passive reference dye is pre-added ■ By adding UNG (Uracil-N-Glycosylase) separately, carryover prevention treatment is possible (UNG is not included in this product) *For more details, please refer to the PDF document or feel free to contact us.

• others

This product is a kit for extracting DNA from soil and fecal samples. It combines chemical lysis using surfactants with physical cell disruption through Beads Beating. The method employs a spin column equipped with a DNA recovery membrane, allowing for simple and rapid DNA extraction. 【Features】 ■ Combination of chemical lysis and physical cell disruption ■ Capable of extracting DNA from microorganisms with robust cell walls using Beads Beating ■ No need for nucleic acid preparation through ethanol precipitation, enabling simple and rapid DNA extraction *For more details, please refer to the PDF document or feel free to contact us.

• others

【Features】 ■ Short delivery time ... Next-day delivery anywhere in the country ■ Convenience ... 10nmol guarantee with no dilution needed for immediate use ■ High quality ... Reverse phase column purification is standard grade We can deliver the next day anywhere in the country after you place your order! All synthesis is handled in domestic labs, and there are no shipping fees for orders of one bottle. We standardly support ready-to-use concentrations such as 5μM and 10μM. After delivery, you can use it directly in experiments without any dilution, making it very convenient. We adopt reverse phase column purification for standard purification grade, and quality inspections are conducted for all products using "TOF-MASS" or "capillary electrophoresis"! *For more details, please refer to the PDF document or feel free to contact us.

• Contract manufacturing

We would like to introduce our in-house data on soil samples. This data consists of the results of analyzing four samples from four locations. Approximately 100,000 reads have been obtained for each sample using MiSeq. The delivered data includes files in HTML format, and graphs can be viewed in a web browser, so please check them out via the related links. 【Overview】 ■ Results of microbial community structure analysis using the V4 region of 16S rRNA (classification at the phylum level) ■ OTU heatmap ■ Principal Coordinate Analysis (PCoA) *Optional ■ Rarefaction curve *Optional *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Analysis

We will introduce community structure analysis using the selective membrane permeable dye EMA (in-house data). For environmental samples dominated by E. coli, we prepared samples that were heat-treated and sterilized, as well as untreated samples, and performed EMA treatment. Subsequently, DNA extraction was conducted for each sample, followed by 16S rRNA gene amplicon analysis. As a result, in the community structure analysis, E. coli, which had become dominant, was hardly detected in the heat-treated samples due to its death. Additionally, in the related links, we also introduce community structure analysis using reverse transcription cDNA from RNA (in-house data), so please take a look. 【Workflow】 ■ Prepare environmental samples (heat-treated, untreated) ■ EMA treatment ■ Light irradiation ■ DNA extraction ■ Creation of adapter library via EMA-PCR *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

At the Kyoto University Center for Ecological Research, research is being conducted on the diversification of the genus Acer and the processes involved in coexistence among species. Our company has been commissioned to conduct part of the analysis, which we would like to introduce. In this analysis, we utilized host-specific blocking primers to suppress the amplification of host-derived genes, allowing for the efficient identification of the host, the leafroller moth, and a comprehensive exploration of parasites within it. We found that the analytical method using blocking primers is an effective means of elucidating the relationships between hosts and parasites that are difficult to detect visually. 【Case Overview】 ■ PCR was conducted in the presence and absence of blocking primers. ■ COI amplicon analysis was performed on 274 samples of leafroller moth larvae using Miseq. ■ From the obtained reads, we identified 10 species of leafroller moths and 13 species of parasitic wasps, clarifying the host-parasite relationships. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce an example analyzed using MiFish, which is used for DNA metabarcoding of fish. Water samples were collected from multiple locations in the Sagami River system and pooled in a plastic tank. To prevent DNA degradation, 1 ml/L of Osban disinfectant was added immediately after sampling. The samples were brought back at room temperature, and filtration and DNA extraction were performed in the lab. The obtained data was provided to MitoFish for homology search. As a result, it was observed that the number of fish species detected increased, suggesting that PCR repetition is effective in increasing the number of detected fish species. [Case Overview] - Filtration and DNA extraction were performed in the lab. - NGS library preparation was conducted using the extracted DNA. - Library preparation was performed in 8 repetitions. - The prepared library was sequenced using Illumina's MiSeq. - The obtained data was provided to MitoFish for homology search. *For more details, please refer to the PDF document or feel free to contact us.*

• Contract Analysis

Genome editing technology is a powerful tool that enables free genetic modification, but genotype analysis (genotyping) is an important step in selecting modified individuals. By evaluating allele frequencies in the F0 generation through next-generation sequencing, it is possible to select individuals for breeding. Additionally, in genome editing experiments with cultured cells, a diverse population of cells with various mutations can be obtained from a single experiment, and the nature of the mutations varies depending on the experimental conditions. Next-generation sequencing allows for detailed evaluation of the mutations in the cell population. [Case Overview] - Genotyping of cultured cells using CrispRvariant - After extracting DNA from cultured cell lines edited with CRISPR/Cas, target amplicon sequencing was performed using Miseq - The obtained data was analyzed using CrispRvariant *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

In our company, when preparing amplicon samples aimed at analyzing the microbial community structure present in plant leaves, we add PNA to suppress amplification from plant mitochondrial and chloroplast origins, resulting in favorable outcomes. Without the addition of PNA, approximately 90% of the acquired data consists of amplification products derived from the host. By adding PNA, we can reduce this to about 10%, allowing for a higher resolution estimation of the target organisms. [Results of condition testing with 4 sample units] ■ Ct1-Ct4: No PNA added ■ Mt1-Mt4: PNA added for mitochondrial blocking ■ Pl1-Pl4: PNA added for chloroplast blocking ■ PM1-PM4: PNA added for both mitochondrial and chloroplast blocking *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

From the Ocean Research Institute, we received new marine proteobacteria from the Deep Sea and Crustal Biosphere Research Division, and we would like to introduce the results of our analysis. Using a next-generation sequencer (MiSeq), we performed sequencing analysis and obtained paired-end reads. After quality filtering the raw reads, we conducted assembly and further predicted genes from the draft genome sequence. 【Case Summary】 ■ Raw Reads: 7.3M paired-end reads (2x300bp) ■ Annotation Data - Number of Genes: 2,417 - rRNA: 6 - tRNA: 49 *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

MLST (Multi-locus sequencing typing) analysis is a method for typing bacteria by patterning mutations in multiple gene regions (usually more than seven). In this analysis case (in-house data), samples were prepared assuming the identification of isolates' genomes and environmental bacteria (samples mixed with environmental DNA and the genomes of isolated strains), and typing was conducted. The prepared strains included Gram-positive and Gram-negative bacteria, and genome sequencing and typing were performed on these samples using Miseq. As a result, this method allowed for the identification of strains mixed with environmental DNA. [Case Overview] - Samples were prepared assuming the identification of isolates' genomes and environmental bacteria, and typing was conducted. - The prepared strains were Bacillus subtilis subsp. subtilis str. 168 (Gram-positive) and Pseudomonas protegens Pf-5 (Gram-negative). - Genome sequencing and typing were performed on these samples using Miseq. *For more details, please refer to the PDF document or feel free to contact us.*

• Contract Analysis

"Target Amplicon Analysis" is a service that can be used reasonably by packaging it. The target regions for analysis include 16S V4, 18S V9, ITS (gITS7-ITS4), COI (mlCOIintF-HCO2198), and MiFish (12S V5), among others. We provide primers for sample preparation targeting the amplification regions free of charge, and you will send us your samples. After that, we will use QIIME and other tools to visualize the data as "microbial community composition graphs" and "heat maps." [Optional Services (Excerpt)] - Change of target region length (approximately 400-500 bp) - Library mix preparation - Rarefaction curve and principal coordinate analysis (PCoA) - OTU BLAST analysis *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

The "Microbial Genome Draft Analysis Package" is a service that can be used reasonably by being packaged. Acceptable samples include bacteria and archaea. As optional services, we offer auto-annotation using Prokka, BLAST analysis of contigs, and sequence type typing. Please feel free to contact us when you need our services. 【Analysis Overview】 (1) Customer: Send the extracted genomic DNA to us. (2) Our company: Perform QC work on the received samples. (3) Our company: Prepare the adapter library for MiSeq analysis. (4) Our company: Conduct a 2×300 bp run on MiSeq. (5) Our company: Assemble the acquired data and create contigs. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

In our "Next Generation Sequencing" services, we offer various plans tailored to meet our customers' objectives, and we strive to provide proposals that align with their requests. In the example of "Wildlife Diet Analysis," we directly analyze PCR products using MiSeq, similar to the analysis of gut microbiota. Specifically, we analyze the COI gene, which is used for animal barcoding in the Japan Barcode of Life Initiative, using digestive tract contents or feces as samples to estimate the prey organisms. [Case Overview] ■ In the analysis using MiSeq, we directly analyze PCR products, similar to the analysis of gut microbiota. ■ Specifically, we conduct COI gene analysis using digestive tract contents or feces as samples to estimate the prey organisms. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

In our "Next Generation Sequencing" services, we offer various plans tailored to our customers' objectives and strive to provide proposals that meet their requests. In the example of "microbiome analysis," we directly determine the nucleotide sequence of PCR products as the target for analysis, typically yielding around 10,000 data points. Furthermore, we can obtain data from minor groups that are very difficult to acquire using the clone library method. [Case Overview] ■ Direct determination of nucleotide sequences using PCR products as the target for analysis ■ The data obtained usually exceeds 10,000 nucleotide sequences ■ Data from minor groups can be obtained *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Analysis

Here are some examples of the use of the genome editing tool "GenomeCraft." In the case of "double knockout (zebrafish)," Cas9 protein was mixed with sgRNA targeting two genes or GENOME CRAFT Type CT, and injected into zebrafish embryos to observe the development. One day after fertilization, abnormalities in heart formation stained with GFP were observed, and two days later, abnormalities in eye pigment formation were noted. Additionally, there are examples of use in "in vitro digestion assay," "in vivo genome editing," and "gene knock-in (mice)." [In vitro digestion assay use case] ■ Experiment details - Cas9 protein was mixed with sgRNA or crRNA + tracrRNA, along with substrate DNA fragments, to conduct DNA cleavage experiments. ■ Results: A shift in the band was confirmed, indicating that both sgRNA and crRNA + tracrRNA exhibited good DNA cleavage activity. *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services

At Fasmac, since our establishment in 2001, we have been advancing the development of "Japanese Standard Analytical Methods" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare, utilizing molecular biological techniques. The developed testing technologies are available not only in Japan but also in the United States, China, and other countries. Additionally, since our establishment, we have been actively engaged in activities such as the international standardization of "food inspection methods using molecular biological techniques," and our technical capabilities are internationally recognized. 【Business Activities】 ■ Food inspection and various contract services ■ Manufacturing and sales of food inspection reagents, manufacturing and sales of reagents for genetic engineering *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Inspection
• Other contract services

"GenomeCraft Cas9" is a Cas9 protein derived from Streptococcus pyogenes, equipped with nuclear localization signals at both the N-terminus and C-terminus. We offer two types of Cas9 proteins commonly used in genome editing via CRISPR/Cas: the double-strand cutting "Cas9 protein" and the single-strand cutting "Cas9 D10A nickase," which only cleaves the antisense strand of the target sequence (the complementary strand of PAM nGG). These can be used for genome editing through microinjection, electroporation, lipofection, and other methods, as well as for in vitro DNA cleavage experiments. 【Product Lineup】 ■ Cas9 Protein ・GE-005-S (Quantity: 100μg) ・GE-005-L (Quantity: 100μg x3) ■ Cas9 D10A Nickase ・GE-006-S (Quantity: 100μg) ・GE-006-L (Quantity: 100μg x3) *For more details, please refer to the PDF document or feel free to contact us.

• Other information systems

The CRISPR/Cas9 system, which is rapidly spreading as a genome editing technology, requires specific RNA for each target sequence. With Phasmac's "GENOME CRAFT RNA," you can easily achieve genome editing by simply mixing it with your existing Cas9 and introducing it into cells. There is no need for cumbersome expression vector construction or careful RNA preparation tasks anymore. Please make use of this to improve the efficiency of your genome editing work. 【Features】 ■ Approach more targets more easily ■ No need for expression vector construction or careful RNA preparation tasks ■ Simply mix with your existing Cas9 and introduce it into cells for easy genome editing ■ Functions as guide RNA for the CRISPR/Cas system using chemically synthesized crRNA and tracrRNA *For more details, please refer to the PDF materials or feel free to contact us.

• Other information systems

At Fasmac, we offer STR analysis (for cell authentication). We conduct STR analysis using the GenePrint24System (from Promega). After performing multiplex PCR with the kit, we carry out capillary electrophoresis to determine the STR genotypes at 24 specific locations, including CODIS and ESS loci, as well as Y chromosome loci. The allele data we report can be used for cell authentication by cross-referencing with public databases. 【Details】 ■ Delivery time: 1 week ■ Items to be sent - DNA sample (20ng or more) *Please send it via refrigerated or frozen shipping - Printed order sheet ■ Deliverables - Allele data (Excel), waveform data (PDF/fsa file) *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services