ジーンフロンティア List of Products and Services

Antibody artisans conduct selection in vitro against fixed antigens without using animal immunity. This allows for binding reactions to be performed in the state or environment of prepared peptides or proteins, enabling precise antigen design, such as narrowing down the area of interest. Here, we present an example of antibody production using peptide antigens to obtain antibodies that recognize specifically phosphorylated proteins at certain Ser residues. We invite you to read on. [Contents] 1. Preparation of Antigens 2. Selection to Increase Specificity 3. Selection of Antibodies 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

Antibody artisans perform selection in vitro against fixed antigens without using animal immunity. As a result, it is possible to conduct binding reactions in the state or environment where peptides or proteins are prepared, allowing for detailed antigen design, such as narrowing down the area of interest. Here, we will introduce an example of producing an antibody that can distinguish a substitution of one amino acid within a specific sequence. We encourage you to read on. [Contents] 1. Preparation of Antigens - 1-1. Narrowing down the target area - 1-2. Setting up antigens including negative controls - 1-3. Synthesizing peptides 2. Selection to Increase Specificity - 2-1. Excluding antibodies that bind to ends, etc. - 2-2. Increasing selection pressure with multiple antigens 3. Selection of Antibodies - 3-1. Checking the signal-to-noise ratio with ELISA - 3-2. Confirming the nucleotide sequence of the antibody 4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

DJ-1 is a 21 kDa protein, and we present a successful case of producing antibodies that specifically recognize the oxidized form of the Cys residue at position 106 (C106) of DJ-1. We have attempted to obtain antibodies that specifically recognize the oxidized C106 through the production of polyclonal antibodies via immunization in rabbits and monoclonal antibodies via immunization in mice, but we were unable to obtain the desired antibodies in either case. Here, we introduce a method for producing antibodies that do not involve animal immunization and are selected entirely in vitro, along with the results of verifying the specificity of the obtained antibodies. 【Contents】 ■1. What is DJ-1 ■2. Strategic Screening 　・2-1. Antigen Design 　・2-2. Panning and ELISA Screening 　・2-3. Cross-reactivity Confirmation by ELISA ■3. Western Blotting and Immunoprecipitation ■4. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF document or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

Antibody artisans produce antibodies entirely in vitro without using animal immunization, allowing for detailed antigen design, such as narrowing down sequences and confirming cross-reactivity with different antigens during selection. In this case, we will introduce the antigen setup and selection method for producing antibodies that belong to a family of target proteins, binding only to the desired protein and not to other proteins within the same family. 【Contents】 ■1. Specificity of antibodies ■2. Preparation of antigens 　・2-1. Design of antigens 　・2-2. Production of conjugates ■3. Methods of selection 　・3-1. Panning 　・3-2. Screening ELISA ■4. Key points of selection 　・4-1. Design of antigens 　・4-2. Enhancing antibody specificity ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

When proteins undergo processing and are divided into multiple fragments, it becomes very important to set the antigens for producing antibodies that recognize each fragment. Antibody artisans perform all selections in vitro without using animal immunization, allowing them to conduct binding reactions with peptides or proteins in their prepared state, without the steps of fragmenting proteins for antigen presentation. In this article, we will introduce the antigen setting and selection methods for producing antibodies that can distinguish between fragments before and after cleavage by a protease of a certain protein. 【Contents】 ■1. Specificity of antibodies ■2. Design of antigens ■3. Screening methods 　・3-1. Panning 　・3-2. ELISA screening ■4. Protein antigens and peptide antigens 　・4-1. Which one should be chosen? 　・4-2. Innovations in selection ■5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

In cases where a protein with a single amino acid mutation exists in a mixed state alongside the wild-type sequence, there may be a need for antibodies that can specifically distinguish this mutated protein. Specifically, as an antigen, a peptide synthesized from the mutated sequence portion is used, and through panning via phage display and ELISA screening, clones that are negative for the wild-type and positive for the peptide with the single amino acid mutation are selected. I will introduce each step. [Contents] 1. Design and coupling of peptide antigens 2. Panning 3. ELISA screening 4. Sequencing 5. Selection of candidate antibodies *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

We will introduce a case of obtaining antibodies that inhibit activity, which could not be achieved through animal immunization, using the phage display method called "Antibody Craftsman," specifically in the case of autotaxin. The reason why inhibitory antibodies against autotaxin (ATX) could not be obtained through animal immunization is thought to be due to the high conservation of ATX across species, making it difficult to establish inhibitory antibodies targeting the catalytic active site. Therefore, in the Antibody Craftsman approach, we intentionally selected antibodies that recognize both human and mouse autotaxin (ATX) and developed a strategy to select inhibitory antibodies from among them. 【Contents】 1. What is Autotaxin 2. Structure of Autotaxin 3. Strategic Screening - 3-1. Panning - 3-2. ELSIA Screening - 3-3. Enzyme Activity Inhibition Screening 4. Activity Measurement Using Antibodies 5. Summary *For detailed content of the article, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

The monoclonal antibody production service by Antibody Craftsman utilizes a unique antibody production technology that does not rely on animals, allowing for in vitro selection to narrow down the regions of antigens that antibodies bind to and to create antibodies that can distinguish slight differences in antigens. Here, we introduce some of the selection methods used to obtain the desired Fab antibodies. Additionally, please take a look at our achievements in antibody production. [Content Included] ■ Antibodies that identify post-translational modifications ■ Antibodies that specifically recognize homologous regions ■ Antibodies against membrane proteins ■ Antibodies that distinguish amino acid substitutions and deletions ■ Antibodies that recognize three-dimensional structures ■ Antibodies with strict specificity *For detailed content, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

This article introduces the use of immunoglobulins for drug and diagnostic development. It includes topics such as "Use as Human Antibody Reference and Positive Control," "Chimeric Surrogate Antibodies," "Pharmacokinetic Analysis Tools," and "Human IgG Standard Samples for Immunogenicity Testing." Please take a moment to read it. [Contents] ■ Use as Human Antibody Reference and Positive Control - IgA for HuCAL reference samples in anti-phospholipid antibody syndrome ELISA ■ Chimeric Surrogate Antibodies ■ Pharmacokinetic Analysis Tools - High-sensitivity pharmacokinetic assay systems using HuCAL antibodies for drug discovery ■ Human IgG Standard Samples for Immunogenicity Testing - HuCAL IgG1 standard samples in homogeneous bridging assays *For more details, you can view the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

On this homepage, we introduce the use of HuCAL antibodies. Antibodies produced using HuCAL technology (HuCAL antibodies) can be used in various applications just like antibodies produced by conventional methods. Furthermore, as they are Fab antibodies, they possess properties that can provide additional advantages in certain assay systems, such as preventing the binding of the Fc region to receptors and eliminating the need to remove the Fc region to minimize human anti-mouse antibody (HAMA) reactions. Please take a look at our homepage. 【Features】 ■ Can be used similarly to antibodies produced by conventional methods ■ Prevents the binding of the Fc region to receptors ■ No need to remove the Fc region to minimize human anti-mouse antibody reactions ■ Possesses properties that can provide additional advantages in certain assay systems ■ Tag-specific detection antibodies are very useful in many applications *For detailed information, please refer to the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

This article introduces examples of monoclonal antibody production. The antibody craftsman's monoclonal antibody production service utilizes a unique antibody production technology that does not rely on animals, allowing for a high degree of flexibility in setting antigens. This enables the production of antibodies that can recognize slight differences in antigens or have specific binding sites tailored to the purpose, resulting in antibodies with special specificities that meet specific needs. HuCAL technology has over 10 years of proven results worldwide, having produced 14,000 antibodies. Its success rate over the past three years boasts over 90%, and it is adopted by many pharmaceutical companies and others. [Contents] ■ Examples of monoclonal antibody production (with work overview) ■ Other antibody production examples *Detailed content of the article can be viewed via the related links. *For detailed specifications, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services
• Contract manufacturing

Question: How long does it take to produce antibodies? Answer: It takes at least 7 weeks from the start of antigen screening. It is possible to produce them in a shorter period compared to the monoclonal antibody production method using mice. *For more detailed information about the service, please refer to the PDF document or feel free to contact us.*

• Other services
• Other contract services

Question: What is the difference from other phage display methods? Answer: The concept and methods for creating the library are different. We analyze the structure and sequences of human antibodies and construct a high-quality library artificially synthesized from DNA according to the rules of human antibodies. *For more detailed information about the service, please refer to the PDF document or feel free to contact us.*

• Other contract services
• Other services

Question: Does the phage display method for antibody production use animals? Answer: Yes, it does not. The phage display method uses E. coli and phages that infect it to produce antibodies. AbD Serotec, A Bio-Rad Company, uses phage display technology to artificially create human-like monoclonal Fab antibodies. *For more detailed information about the service, please refer to the PDF document or feel free to contact us.*

• Other services
• Other contract services

Production of two types of antibodies (sandwich pair) with different antigen determinants (epitopes). The antibodies obtained through the Antibody Artisan's contract services come with various optional services. The sandwich method serves as the foundation for many research and diagnostic assay systems. At Antibody Artisan, we provide methods to discover the best-matching antibody pairs. For pricing, please inquire for confirmation. *This is an optional service for antibodies produced by Antibody Artisan. *The menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services

Format Conversion The antibodies obtained through the Antibody Artisan's contract services come with various optional services. If an Fc region is required, we can convert the candidate antibody's Fab into full-length human, human-mouse chimeric, or human-rat chimeric forms, and deliver purified IgG molecules within 10 weeks. After cloning the genes for the VH and VL regions along with the desired constant region into a vector, we simultaneously introduce the light chain and heavy chain vectors into mammalian cells for expression. For more details, please also refer to the selection of antibody formats in the service flow. For pricing, please inquire for confirmation. *This is an optional service for antibodies produced through the Antibody Artisan service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

• Other contract services
• Other services

Production of various tags and fusion proteins The antibodies obtained through the contract services of Antibody Craftsman come with various optional services. Since the HuCAL gene is modular, it allows for a wide range of antibody formats to be selected, and it is also possible to add various tags or produce fusion proteins with different partners. Once candidate antibodies are determined, it is easy to change the Fab format or convert to another immunoglobulin isotype. For combinations of formats and tags, please refer to the product manual "Fab Antibody Format and Tag Combinations" available on the download site. For pricing, please inquire for confirmation. *These are optional services for antibodies produced through the Antibody Craftsman service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services

Improvement of Affinity After Antibody Production The antibodies obtained through the Antibody Artisan's contract services come with various optional services. The Antibody Artisan offers services to further enhance the affinity of the produced antibodies. In the HuCAL PLATINUM library, each CDR region can be freely replaced like a cassette, and by combining this with high-speed screening technology, it is possible to rapidly improve the affinity of antibodies by 10 to 1,000 times. Please inquire for pricing details. *This is an optional service for antibodies produced through the Antibody Artisan service. *This menu is not applicable to antibodies you already possess. *For more details, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services

In addition to the menu below, we can provide proposals tailored to your requests, so please feel free to consult with us. List of Optional Services ■ Affinity enhancement after antibody production ■ Production of various tags and fusion proteins ■ Format conversion ■ Production of sandwich pairs Please inquire for confirmation regarding the prices listed above. *For more details, please refer to the PDF document or feel free to contact us.

• Other services
• Other contract services

The candidate antibodies and final antibody clones produced by the antibody craftsman will be stored for 5 years (extendable). We will accept additional production of the selected antibodies at the following prices (from mg to g orders). Antibody production setup Desired selling price for His-tag antibody: ¥80,000 Desired selling price for Strep-tag antibody: ¥114,000 Per 1 mg of antibody production Desired selling price for His-tag antibody: ¥70,000 Desired selling price for Strep-tag antibody: ¥70,000 *For more details, please refer to the PDF document or feel free to contact us.

• Other services
• Other contract services

Antibody Production Pricing Standard Antibody Production = ¥1,800,000 per antigen Provision of 1 to 5 candidate antibodies (200μg each) from which the customer can select the final antibody, provided at 1mg (Myc-His tagged, ELISA positive). Additional Sequencing = ¥50,000 per 10 clones Additional sequencing for the same antigen. *The standard price includes sequencing for 10 clones. Additional Candidate Antibodies = ¥50,000 per candidate antibody Additional candidate antibodies for customer confirmation against the same antigen (200μg each). *The standard price includes delivery of up to 5 clones. Additional Final Antibody Production = ¥70,000 per 1mg Additional production of final antibodies for the same antigen simultaneously. *The standard price includes delivery of 1 clone (1mg). *For more details, please refer to the PDF document or feel free to contact us.

• Other contract services
• Other services

The Antibody Craftsman's monoclonal antibody production service utilizes unique technologies such as phage display, strategic antibody selection, and high-throughput processes to select specific Fab antibodies against various antigens. This process enables us to provide antibodies with specialized specificities tailored to our customers' needs and to prepare antibody formats in a short period. HuCAL PLATINUM Library The HuCAL PLATINUM, consisting of approximately 45 billion patterns, is the world's largest high-quality antibody library. This library is based on a combination of genes for complementarity-determining regions (CDRs) with high diversity and framework master genes, optimized for expression in E. coli. *For more details, please refer to the PDF materials or feel free to contact us.

• Other services
• Other contract services

HuCAL technology is an antibody production technique that does not rely on animals and conducts all selections in vitro. Specifically, antibodies are presented using phages that incorporate human antibody genes, allowing them to bind to antigens. Since the presented antibodies (Fab antibodies) correspond one-to-one with the genes that code for them in the form of phage particles, it is easy to identify the genes of the Fab antibodies that bind to the antigen from among many Fab antibodies (phage display). A solution containing phages is added to a plate with immobilized antigens, allowing the phages to bind through the presented antibodies. Phages that did not bind to the antigens are washed away, and the bound phages are then eluted and collected. By repeating this process several times, phages that bind more strongly to the antigen can be concentrated (panning). The antibody genes encoded by the phages obtained through panning are expressed in E. coli, and Fab antibodies are purified. *For more details, please refer to the PDF materials or feel free to contact us.*

• Other services
• Other contract services

In the antibody artisan's monoclonal antibody production service, we produce monoclonal antibodies using the HuCAL technology developed by MorphoSys and owned by Bio-Rad (formerly AbD Serotec, A Bio-Rad Company). HuCAL (Human Combinatorial Antibody Library) is a unique library of recombinant Fab antibodies that encompasses the diversity of human antibodies and is developed as a high-quality library to produce highly specific artificial antibodies against various types of antigens. The HuCAL technology is based on the HuCAL PLATINUM library and CysDisplay technology developed by MorphoSys, and it is utilized by many customers worldwide as a very powerful tool for the production of fully human antibodies for research, diagnostics, and therapeutic purposes. *For more details, please refer to the PDF document or feel free to contact us.*

• Other contract services
• Other services

For features 1 to 3, please refer to the seven characteristics of our monoclonal antibody production contract service. 4. Customization through molecular engineering It is possible to add various tags to antibodies or produce various fusion proteins. Additionally, dimerization can be achieved using a helix-turn-helix motif, which can enhance antigen cross-linking ability and improve affinity. 5. Abundant options As an option, we can also handle the preparation of antigens. Conversions to human IgG, IgA, or mouse chimeric IgG, as well as affinity enhancement after antibody production, increasing the number of candidate antibodies, and additional antibody preparations are possible. 6. No cost if ELISA-positive antibodies are not obtained Our service boasts a success rate of over 90%. Based on this confidence backed by our success rate, there will be no charge if ELISA-positive antibody clones are not obtained against the antigen. 7. Continuous supply of antibodies Antibodies are backed up in three formats: whole cells, plasmid DNA, and DNA sequence information, ensuring that all antibodies can be rapidly reproduced from these backups, guaranteeing a continuous supply. *Please feel free to contact us for more details.

• Other contract services
• Other services

1. Strategic Selection By using control antigens for strategic selection, it is possible to produce antibodies with special specificities, such as those that recognize the presence or absence of post-translational modifications, or antibodies that recognize common parts of homologous proteins. 2. Production of Antibodies Difficult to Obtain through Animal Immunization Since it does not rely on animal immunization, it is possible to produce antibodies against human or mouse antigens regardless of immune tolerance. Additionally, it is possible to produce antibodies against toxic antigens or immunosuppressive substances. Furthermore, since there is no antigen presentation step, it is also possible to produce antibodies that recognize natural three-dimensional structures. 3. Delivery of Candidate Antibodies in Just 7 Weeks Thanks to automated screening of antibody libraries, automated sequencing, and automated ELISA analysis, the time required from receipt of the antigen to delivery of candidate antibodies (up to 5) is just 7 weeks. For points 4 to 7, please refer to the seven features of the monoclonal antibody production contract service in Feature 2. *For more details, please refer to the PDF document or feel free to contact us.

• Other contract services
• Other services

The custom monoclonal antibody production service "Antibody Craftsman" provided by Gene Frontier utilizes a unique antibody production technology (human Fab antibody library and phage display) that does not rely on animals, allowing for the creation of antibodies that can recognize the presence or absence of post-translational modifications and differences in amino acids, which is a significant feature. The HuCAL technology, which uses the human Fab antibody library and phage display, has over 10 years of proven results worldwide, having produced 14,000 antibodies. In the past three years, the success rate has boasted over 90%, and it has been adopted by many pharmaceutical companies and others. For example, we can produce antibodies such as: ■ Antibodies that identify post-translational modifications ■ Antibodies that specifically recognize homologous regions ■ Antibodies that identify substitutions or deletions of a few amino acids ■ Antibodies that recognize denatured proteins If you consult us about the specificity and usage of the desired antibody, we will propose a planning strategy for antibody production, including antigen design, selection methods tailored to the type and application of the antigen, and choices regarding the format and tags of the antibody. *For more details, please refer to the PDF materials or feel free to contact us.

• Other contract services
• Other services

Among the three Cys residues present in the DJ-1 protein, which is said to be involved in Parkinson's disease, we attempted to obtain antibodies that specifically recognize only the oxidized DJ-1 with the 106th Cys. This was done through the production of polyclonal antibodies via immunization in rabbits and monoclonal antibodies via immunization in mice, but we were unable to obtain the desired antibodies. Therefore, we used a peptide synthesized based on the surrounding sequence that includes the oxidized C106 as the antigen, and created conjugates with different carrier proteins and cross-linkers. As a result, we were able to identify clones that showed positive results for the oxidized C106 peptide and negative results for the reduced C106 peptide. [Case Summary] <Problem> ■ The methods of producing polyclonal antibodies through immunization in rabbits and monoclonal antibodies through immunization in mice did not yield the desired antibodies. <Result> ■ We were able to identify clones that showed positive results for the oxidized C106 peptide and negative results for the reduced C106 peptide. *For more details, please refer to the PDF document or feel free to contact us.

• Other services
• Other contract services

With "Antibody Craftsman," selection is performed in vitro against fixed antigens without using animal immunization. This allows for binding reactions to be conducted in the state or environment of prepared peptides or proteins, enabling detailed antigen design, such as narrowing down specific areas. This document is a must-read for those who want to learn more about the selection of antibodies that can distinguish a single amino acid substitution, so please feel free to take a look. [Contents (excerpt)] - Preparation of antigens - Selection to increase specificity - Selection of antibodies *For more details, please refer to the PDF document or feel free to contact us.

• Research Antibodies

This document is about the contract service for producing monoclonal antibodies called "Antibody Craftsman," which conducts all selections in vitro without using animal immunity. It allows for binding reactions to be performed with peptides or proteins in their prepared state, without processes such as fragmenting proteins for antigen presentation. As a result, it enables detailed antigen design, such as narrowing down sequences. In this instance, we will introduce the antigen setup and selection method for producing antibodies that can distinguish between fragments before and after cleavage by a certain protease. 【Contents】 ■ Specificity of the antibody ■ Design of the antigen ■ Screening methods ■ Protein antigens and peptide antigens ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

• Other contract services

"Can it really be synthesized?" "It seems like the synthesis amount is small..." "This is my first time with a cell-free system..." Such anxieties and concerns can be resolved! We provide full support. With PUREfrex2.0 mini, which includes common primers for all proteins, please first try to see if your protein can be synthesized. ■ What is PUREfrex? PUREfrex is a kit that reproduces the protein synthesis reaction that occurs inside cells, such as E. coli, in a tube. By adding the gene of the protein you want to synthesize to the kit and reacting it at 37°C for 4 hours, the desired protein will be synthesized. It is recommended for when you want to try a little during preliminary experiments or when you need multiple proteins. You can confirm the synthesis amount with a small volume of reaction solution, and then increase the volume of the reaction solution to synthesize the required amount. 【Features】 ■ Synthesis is possible on a scale from a few μL to several tens of mL ■ Additive selection can be customized according to the protein being synthesized ■ Bulk orders are also supported (hundreds of mL to L) ■ Customization of the reaction solution is also available For more details, please download the PDF or feel free to contact us.

• Protein Extraction Kit
• Other purification and extraction

PUREfrex is a kit that reproduces the protein synthesis reactions occurring in cells such as E. coli within a tube. By adding the gene of the protein you want to synthesize to the kit and allowing it to react at 37°C for four hours, the desired protein is synthesized. It is recommended for when you want to try a small experiment or when you need multiple proteins. You can confirm the synthesis amount with a small volume of reaction solution, and then increase the volume of the reaction solution to synthesize the required amount. 【Features】 ■ Synthesis is possible on a scale from a few μL to several tens of mL ■ Additive selection can be tailored to the protein being synthesized ■ Bulk orders are also accommodated (from several hundred mL to L) ■ Customization of the reaction solution is available For more details, please download the PDF or feel free to contact us.

• Other research reagents
• Protein Extraction Kit
• Other purification and extraction

Are you having trouble with the low specificity of conventional monoclonal antibodies? With "Antibody Artisan," you can produce antibodies targeting specific regions, resulting in antibodies with higher specificity. For example, we can produce antibodies such as: ■ Antibodies that identify post-translational modifications ■ Antibodies that specifically recognize homologous regions ■ Antibodies that distinguish between a few amino acid substitutions or deletions ■ Antibodies that recognize denatured proteins "Antibody Artisan" employs a unique technology (a library of 45 billion types of artificial human antibodies and phage display) that does not use animal immunization, allowing us to design antigens and select strategically. All work is done in vitro, enabling us to deliver antibodies as quickly as seven weeks after receiving the antigen. Additionally, the antibodies obtained through this service can be expressed and purified as recombinant proteins in E. coli, allowing for repeated production of additional antibodies. Antibody clones are stored for five years, and sequences are kept permanently. *For more details, please refer to the PDF materials or feel free to contact us.

• Research Antibodies
• Other contract manufacturing
• Flow cytometer