バイオタージ・ジャパン List of Products and Services

The nanofibers developed using two types of cellulose and non-cellulose, which differ from conventional porous resins, enable the purification of large molecules. They possess high binding capacity even at high flow rates, allowing for process shortening. This makes efficient purification possible and contributes to the removal of impurities such as HCP. pDNAHERO binds to the nanofibers using weak HIC as a ligand, showing affinity for supercoiled plasmids. Long-chain plasmids can also be used. Additionally, purification of single-stranded plasmid DNA is possible, and it can be utilized in the process of mRNA synthesis via IVT.

• Refining and Extraction Equipment
• Other Chromatograph
• Process Development

In the world of bioprocessing, traditional porous methods used for antibody purification have been mainstream for decades, but they are unsuitable for the purification of large molecules such as lentiviruses, plasmids, and exosomes used in gene cell therapy, making purification difficult. Astrea Bioseparations has developed a next-generation bioprocessing device utilizing cutting-edge nanofiber technology called "AstreAdept." The upcoming nanofibers will be created by electrospinning a blend of two types of polymers, combining cellulose-based and non-cellulose-based materials. Due to their nanoscale size, they have a high surface area-to-volume ratio, making them very attractive. AstreAdept achieves the following: Efficiency: AstreAdept compresses processes, reducing time and costs. Innovative technology: It utilizes nanofibers and convection to enable fast and highly efficient washing. Wide applicability: A scalable solution that can accommodate everything from research to manufacturing. Optimization of exosome purification, bacteriophage, plasmid purification, and the purification of single-stranded pDNA and mRNA before and after IVT.

• Other purification and extraction
• High Performance Liquid Chromatograph
• Chromatographic resins and packing materials

HCPure is a new unique mixed-mode ligand specially designed for the capture of host cell proteins (HCP). It features moderate hydrophobic and polar groups, interacting with proteins through a combination of hydrophobic and electrostatic interactions. Selected from Astrea Bioseparations' Chemical Combinatorial Library, HCPure effectively binds a wide range of host cell proteins from various sources, including bacteria and mammalian cells such as CHO, HEK293, and E. coli, while showing minimal binding to important target biological compounds like monoclonal antibodies and viral vectors. Based on the PuraBead 6HF matrix, HCPure possesses complete alkali stability, allowing for cleaning and sterilization with 0.5M or 1M NaOH, supporting high flow rates, low back pressure, and long operational lifetimes. HCPure is particularly suitable for a wide range of biopharmaceutical polishing processes, especially when the removal of HCP is challenging or when conventional mixed-mode resins are insufficient.

• Other purification and extraction
• High Performance Liquid Chromatograph
• Chromatographic resins and packing materials

An advanced endotoxin removal adsorbent with high affinity for endotoxins, enabling efficient removal of up to 99.9% while maintaining protein yield. EtoxiClear is an affinity resin using patented chemically synthesized ligands, capable of removing up to 99.99% of endotoxins, ensuring safe and high-purity products. EtoxiClear offers powerful advantages over traditional endotoxin removal methods, supporting manufacturers in achieving greater efficiency, reliability, and compliance. Comparison of traditional endotoxin removal methods and EtoxiClear: - Excellent endotoxin removal: Outperforms competitors with a reduction rate of 99.99% - High protein recovery: Superior to ion exchange methods with over 90% recovery rate - Wide range of conditions: Usable at pH 4.0 to 8.0 and various salt concentrations - Fast and efficient: Rapid polishing in flow-through mode with PuraBead matrix - Reusable: Regenerated with 0.5 M NaOH, reducing costs over multiple cycles - Versatile: Suitable for antibodies, vaccines, recombinant proteins, and diagnostic reagents.

• Chromatographic resins and packing materials
• High Performance Liquid Chromatograph
• Other purification and extraction

Until now, plasmid mini-preps required manual operations for purification using a spin method. This PhyTip column can be used with automated pipetting machines, allowing for hands-free elution once only the E. coli pellet is prepared. It can process 96 samples at once. It is compatible with automated pipetting machines such as Beckman i series, Tecan, and Hamilton.

• Other Vectors
• Expression vector
• DNA Purification Kit

Ion exchange chromatography is used as an orthogonal chemistry to obtain high-purity final products, serving as a second polishing step after another purification process or affinity chromatography purification. Ion exchange PhyTip columns are chip-type columns that utilize Dual Flow Chromatography technology, making them suitable for efficient parallel purification of small amounts of protein samples. They are compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. They can accommodate a wide variety of resins and custom packing is also available. Product Information (4 products): Strong Anion exchange Weak Anion exchange Strong Cation exchange Weak Cation exchange

• Separation device

The chip-type gel filtration PhyTip column filled with size exclusion chromatography resin is optimal for desalting protein samples and exchanging buffer, and it also enables high-throughput screening (HTS) in a 96-well plate. Desalting using the gel filtration PhyTip column can be set up as a secondary purification step after affinity purification with an affinity PhyTip column, such as the removal of imidazole after IMAC affinity purification, using the same automated protocol. Like other PhyTip columns, the resin is packed between two hydrophilic screens; however, unlike other PhyTip columns, the gel filtration column passes the sample from the top. The gel filtration PhyTip column has size exclusion resins of 5K and 30K, which retain low molecular weight compounds while allowing molecules of 5 kDa and 30 kDa or larger to pass through. Unlike other PhyTip columns, the use of the ME system is not required. Major liquid handling robots can be used for multi-sample processing. *For more details, please visit our website or feel free to contact us.*

• Separation device

It can be used for protein purification of GST-tagged fusion proteins on a microscale. It is compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting at low speeds, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide variety of resins and custom packing is also available.

• Separation device

Automatic purification of biotin-tagged proteins The Streptavidin PhyTip column filled with a medium that immobilizes streptavidin affinity ligands enables the automatic affinity purification of recombinant biotin-labeled proteins. One of the attractive applications of the Streptavidin PhyTip column is the biotinylated protein assay (immunoprecipitation). It is compatible with major liquid handlers for chip-based chromatography. The use of the PhyTip column requires pipetting operations at low speeds, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide range of resins and custom packing is also available. Custom filling of the desired resin into the chip is possible. *For more details, please visit our website or feel free to contact us.

• Separation device

The IMAC PhyTip column allows for easy affinity purification in small amounts, starting from a minimum resin volume of 5 µL. If you want to process recombinant protein purification in small quantities with multiple samples, it can be handled using HTS. It is compatible with major liquid handlers for chip-type chromatography. The use of the PhyTip column requires slow pipetting operations, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It supports a wide variety of resins and custom packing is also available.

• Separation device

CaptureSelect AAVX Affinity Matrix is specially designed to meet the high selectivity and capacity required for large-scale downstream purification of a wide range of natural and synthetic adeno-associated virus (AAV) serotypes used in gene therapy applications. CaptureSelect AAVX resin has demonstrated binding reactivity against a series of AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, and synthetic serotypes. The PhyTip column is filled with a minimum resin volume of 5 µl, and its unique Dual Flow Chromatography technology allows for the complete process of protein adsorption, washing, and elution to be performed with pipetting operations. It is compatible with major liquid handlers for chip-type chromatography. The use of the PhyTip column requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. It is compatible with a wide variety of resins and custom packing is also available.

• Separation device

This is the PhyTip column ProPlus/ProPlus LX (MabSelect SuRe) using Proplus resin, which outperforms Protein A and Protein G. ▪️ Equipped Technology It features a unique microscale chromatography that employs the patented dual-flow chromatography (DFC), which is the foundational technology of the PhyTip column. Due to its chip-type chromatography, it is compatible with major liquid handlers. The use of the PhyTip column requires pipetting operations at low speeds, so a dedicated electric pipetter, the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide range of resins and custom packing is also available. It is suitable for processing small volumes of multiple samples and can be adapted to major liquid handler robots. A dedicated electric pipetter, the 'ME System', is available for adjustments before transitioning to a smaller number of samples or liquid handlers. If you wish to use a resin not listed in the product list, we can fill the resin for you.

• Separation device

PhyTip is a unique micro-scale chromatography that employs the patented dual-flow chromatography (DFC) technology, which is a foundational technology. It enables micro-scale purification of antibodies, AAV, proteins, and plasmids. The resin can be used from as little as 5 µl, allowing for small-scale protein purification, concentration, and high-throughput applications. Due to its chip-type chromatography, it is compatible with major liquid handlers. The use of PhyTip columns requires low-speed pipetting operations, necessitating specialized electric pipettors like the 'ME System' or liquid handler robots. It supports a wide range of resins and custom packing options. Key PhyTip column products include: ▪ Antibody purification: CaptureSelect PhyTip ProPlus LX (MabSelect SuRe) Columns, etc. ▪ AAV purification: CaptureSelect AAVX ▪ Affinity protein purification: IMAC PhyTip Columns, etc. ▪ Gel filtration ▪ Ion exchange ▪ Plasmid purification (mini prep)

• Separation device

Seamlessly scalable from lab scale to process scale with the FLASH series! Selekt supports column sizes from 5g to 1.5kg. With intuitive operation and assist functions, you can perform LC purification with this single unit. 【Features】 ■ Large 15-inch touch panel for easy visibility ■ Simple operation that requires no manual ■ Integrated design that can accommodate gallon bottles ■ Automatic rack recognition feature ■ QR code reading function for Sfär columns ■ Full wavelength UV spectrum scanning (198 – 400nm or 198 – 810nm) ■ Dedicated optional detector Selekt ELSD offers high sensitivity detection. No clogging with automatic cleaning ■ Connection to external detectors such as ELSD and MS is also possible ■ Purification using preparative LC columns is possible ■ High-speed purification that overturns conventional wisdom ・ Flow rate during purification: Up to 4 times faster (compared to other products. Using Biotage Selekt x Biotage Sfär columns) ・ Flow rate during equilibration: Up to 10 times faster (compared to other products. Using Biotage Selekt x Biotage Sfär columns) ■ Remote operation available (paid option) *For more details, please refer to the catalog or website.

• Separation device
• Analytical Equipment and Devices
• Other physicochemical equipment

Automating analysis preprocessing at a low cost! Compact and desktop-sized with user-friendly operation that requires no manual!

• Dispenser

The "Syro Series (I & II)" can synthesize a large number of peptides in a short time through automatic parallel operation. By minimizing flow paths, it reduces unnecessary consumption of amino acids and reagents, lowering running costs. Additionally, to prevent cross-contamination, each reaction vessel has an independent flow path. 【Features】 ■ More peptides can be synthesized through parallel synthesis ■ Heating up to 95°C is possible (optional) ■ Vortex stirring ■ High-precision dispensing with a syringe pump contributes to high yield and lower running costs *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

The "Initiator+Alstra" is a fully automated peptide synthesis device capable of high-precision dispensing using a syringe pump. The Branches function allows for easy setup of cyclization reactions and side chain synthesis. Efficient microwave heating significantly shortens reaction times. Additionally, touch and edit operations can be performed effortlessly on a large screen. 【Features】 ■ Easy method creation ■ Selectable reactors ■ Microwave synthesis ■ Branches function ■ Choose from different rack sizes *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

PhyPrep is the industry's first large-scale automated robot for purifying plasmid DNA. After approximately 8 minutes of reagent preparation, the device performs plasmid extraction. Like PhyTip, it employs Dual Flow Chromatography (DFC) technology for purification using anion exchange resin. As a result, endotoxin-free and supercoiled plasmids are recovered, achieving high transfection efficiency in plasmid purification. It supports MaxiPrep, MegaPrep, and GigaPrep scales, allowing for the extraction of up to four plasmid samples at once. 【Features】 ■ Transfection-grade ■ User-friendly software ■ Endotoxin-free The necessary reagents are included in the kit in advance. *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

The TurboVap 96 Dual is a dual-channel automatic concentration system designed for plates. It can automatically concentrate two plates simultaneously or independently on the left and right sides. The second generation TurboVap 96 Dual has been completely redesigned in terms of appearance and usability, making it easy for anyone to perform concentration tasks. <Features of the Second Generation TurboVap 96 Dual> - Transparent design allows visibility of the concentration process - Rapid concentration through heated gas spraying! - Convection-type plate heating ensures uniform concentration - Optimization of concentration conditions through adjustments of temperature, gas flow, and plate position - Concentration conditions can be saved as methods (up to 10 types) - Left and right plates can be used under the same or different conditions - Compatible with 24, 48, and 96 positions - Space-saving design allows operation outside of drafts - 40% reduction in nitrogen usage (compared to the previous model)

• Concentration Equipment

The "Biotage Extrahera LV-200" is an automated sample preparation system specialized for trace sample preprocessing. It can process one 96-well plate in about 20 minutes. With smartphone-like ease of use, no programming knowledge is required. Additionally, it achieves reproducible preprocessing through pressurized liquid flow. The dispensing volume ranges from 5 to 800 μL. 【Features】 ■ Specialized for trace sample preprocessing ■ Compatible with 50, 200, and 1000 μL tips ■ Can use trace solid-phase plates ■ Processes one 96-well plate in about 20 minutes ■ Compact design that can be installed on a laboratory bench *For more details, please refer to the PDF document or feel free to contact us.

• Dispenser

The PhyTip column is equipped with the unique technology of Dual Flow Chromatography (DFC), which requires slow pipetting operations. Therefore, a dedicated electric pipetter (semi-automated system) or a liquid handler robot (fully automated system) is necessary. This electric pipetter, the 'ME system', enables the purification of proteins and plasmids using the PhyTip column. With a unique program pre-installed in the pipetter, the performance of the PhyTip column is maximized. It is ideal for setup before introducing it to a liquid handler robot or for processing a small number of samples. 【Features】 ■ Utilizes a wide range of PhyTip column products (antibody purification, IMAC, gel filtration, etc.) ■ Can handle up to 12 samples at once ■ Pre-installed purification programs ■ Automation of each purification step ■ Achieves small volume, high concentration purification *For more details, please visit our website or feel free to contact us.

• Other physicochemical equipment

This application note describes a sample preparation method using mixed-mode weak cation exchange solid-phase extraction for the analysis of three catecholamines (epinephrine, norepinephrine, dopamine) and three metanephrine metabolites (metanephrine, normetanephrine, 3-methoxytyramine) in plasma by LC-MS/MS. The method used here achieves reproducibility and high recovery rates for many common catecholamines in plasma. The EVOLUTE EXPRESS SPE products dramatically improve flow-through properties and enhance the productivity of sample preparation. A simple and rapid Load-Wash-Elute protocol is available for sample preparation, eliminating the steps of column conditioning and equilibration. *You can download the English version of the catalog. *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

Phosphatidylethanol is a highly specific alcohol biomarker, and the concentration of PEth in the blood correlates with alcohol consumption. This application note describes a sample preparation method for three types of PEth in whole blood using the ISOLUTE SLE+ liquid extraction method targeted for LC-MS/MS analysis. ISOLUTE SLE+ liquid extraction plates and columns provide an efficient alternative for the sample preparation of bioanalytical samples compared to traditional liquid-liquid extraction (LLE). High recovery rates are achieved without the formation of emulsions, significantly reducing the effort required for sample preparation. The simple sample preparation operation using ISOLUTE SLE+ allows for clean extracts to be obtained with high reproducibility and good recovery rates. This achieves a lower limit of quantification below 20 ng/mL. This method can be automated using the Biotage Extrahera. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

Vitamin B7 (biotin) is a water-soluble vitamin necessary for cell proliferation. This application note describes a method to achieve reproducible extraction recovery of vitamin B7 in serum while minimizing the co-elution of proteins, lipids, and phospholipids using the EVOLUTE EXPRESS ABN96 well plate. EVOLUTE EXPRESS SPE products dramatically improve flow characteristics, enhance the productivity of sample preparation, and enable clean and robust sample pretreatment. This method can be automated using the Biotage Extrahera. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This application note describes a sample preparation method using polymer-based strong anion exchange mixed-mode solid-phase extraction for the analysis of thyroid hormones T3, rT3, and T4 in serum by LC-MS/MS. Using the EVOLUTE EXPRESS AX 30 mg fixed well plate format, thyroid hormones (T3, rT3, and T4) were extracted from 200 μL of serum. A high recovery rate with good reproducibility was achieved, while minimizing the co-elution of phospholipids, resulting in a quantification limit of less than 50 pg/mL. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

The method described in this application note achieves reproducible and high recovery rates in the analysis of the peptide hormones oxytocin and vasopressin in serum, using the rapid and simple EVOLUTE EXPRESS Load-Wash-Elute protocol. Extraction from serum was performed using the EVOLUTE EXPRESS ABN 96-well plate, and by using a water-rich solvent as the elution solvent, co-elution of proteins, lipids, and phospholipids was minimized. *You can download the English version of the catalog. *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

TSNA, or tobacco-specific nitrosamines, are carcinogenic substances found in tobacco products, including electronic cigarettes and smokeless tobacco. TSNA may show false positives due to secondary and tertiary contamination, similar to nicotine. In methods with sufficiently high detection sensitivity, secondary contamination or passive smoking can cause non-smokers to test positive for TSNA. Tertiary contamination arises from various instruments used throughout the sample pretreatment and analysis process, such as test tubes, solid-phase extraction, pipette tips, and more. ISOLUTE SLE+ retention liquid extraction plates and columns provide an efficient alternative for bioanalytical sample pretreatment, replacing traditional liquid-liquid extraction (LLE). High recovery rates can be achieved without the formation of emulsions, significantly reducing the effort required for sample pretreatment. *You can download the English version of the catalog. *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

This application note describes a sample preparation protocol for the LC-MS/MS detection of the peptide desmopressin in human serum using solid-phase extraction (SPE). Desmopressin is an antidiuretic hormone and a synthetic vasopressin analog. It is clinically used for the treatment of nocturnal enuresis and diabetes insipidus, as well as for coagulation disorders. The method used here achieves reproducible and high recovery rates for desmopressin peptides in serum while minimizing co-elution of proteins, lipids, and phospholipids. The extraction from serum was performed using the EVOLUTE EXPRESS WCX96 well plate. EVOLUTE EXPRESS SPE products provide a robust and efficient high-throughput automated extraction solution. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This application note describes a sample preparation protocol using solid-phase extraction for the detection of three catecholamine metabolites in plasma (vanillylmandelic acid, homovanillic acid, and 5-hydroxyindoleacetic acid) by LC-MS/MS. The method used here achieves high recovery rates with good reproducibility for many common catecholamines in plasma. The EVOLUTE EXPRESS SPE products dramatically improve flow characteristics and enhance the productivity of sample preparation. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This application note describes a sample preparation protocol using a liquid extraction method for the detection of three types of acidic catecholamine metabolites in plasma (vanillylmandelic acid, homovanillic acid, and 5-hydroxyindoleacetic acid) by LC-MS/MS. The method described in this application note demonstrates high linearity (greater than 0.995) in the range of 2 to 200 ng/mL for these target analytes, along with good reproducibility and high recovery rates (over 80%, RSD below 5%). ISOLUTE SLE+ liquid extraction plates and columns provide an efficient alternative for sample preparation in bioanalysis, replacing traditional liquid-liquid extraction (LLE). By using ISOLUTE SLE+, high recovery rates can be achieved without the formation of emulsions, significantly reducing the effort required for sample preparation. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

"PRESSURE+ 48" is a pressurized sample processing manifold compatible with SPE columns in sizes of 1mL, 3mL, and 6mL. With a unique flow restrictor in an individually independent pressurization mechanism, it provides a uniform flow regardless of the sample's viscosity, achieving stable recovery rates. Additionally, samples are collected directly from the SPE column into recovery containers (test tubes). There is no need for cleaning needles or joints, eliminating concerns about contamination. 【Features】 ■ No cock required ■ No power supply needed ■ No joints, preventing contamination ■ Ideal for high-viscosity samples *For more details, please refer to the PDF document or feel free to contact us.

• Other laboratory equipment and containers

The "ISOLUTE FILTER+" is a 96-well filtration filter plate designed to remove fine particles contained in analytical samples and to protect analytical columns and systems from the accumulation of fine particles over time. By layering a coarse depth filter on top of a hydrophilic 0.2μm membrane filter, it achieves good liquid flow characteristics, making it suitable for manual processing using pressure or vacuum, as well as for automated processing using automation systems. The minimum processing sample volume per well is 50μL, with a dead volume of approximately 10μL, allowing for good reproducibility of the collected liquid volume even with small sample sizes. 【Features】 ■ Utilizes a hydrophilic 0.2μm membrane filter ■ Suitable for filtering a wide range of aqueous biological samples ■ Provides good liquid flow characteristics without conditioning ■ Minimal nonspecific binding, usable for a wide range of acidic, basic, and neutral targets with different polarities/hydrophobicities *For more details, please refer to the PDF document or feel free to contact us.

• Other inspection equipment and devices
• Filtration Equipment
• Other filters

The "Biotage Extrahera" is an automatic sample preparation device that emphasizes "ease of use." The software is designed to allow users to freely operate the device. Users can easily create methods and set various conditions, enabling them to execute new sample preparations immediately. It can perform sample preparation tasks such as protein removal, phospholipid removal, non-shaking liquid-liquid extraction (SLE), and solid-phase extraction (SPE) all in one unit. 【Features】 ■ Uses pressurized flow ■ Solvent-resistant touchscreen ■ Capable of recovering up to 3 fractions ■ Uses transparent tips ■ Benchtop unit with exhaust ports ■ Automatic solvent replenishment *For more details, please refer to the PDF document or feel free to contact us.

• Other laboratory equipment and containers

The "V-10 Touch" is a high-speed concentration device that enables rapid solvent evaporation. It removes not only aqueous solutions but also organic solvents up to 40 times faster compared to conventional rotary or centrifugal types, protecting samples from excessive heating and bumping. Furthermore, it can go from liquid to freeze-drying in just 30 minutes. DMSO removal can be completed in only 15 minutes. 【Features】 ■ No pre-connection required ■ Suitable for HPLC fractions ■ Solvent concentration after peptide synthesis ■ Concentration programs are pre-input (editable) ■ Concentration of NMP in 18 minutes, DMSO in 15 minutes (8mL) ■ Samples sprinkled on diatomaceous earth or silica gel can be easily handled with a single touch button! Uniformly at ultra-high speed! ■ Liquid to freeze-drying in 30 minutes ■ Vaporized solvents are effectively trapped within the system *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This document introduces a case study utilizing "PRESSURE+ 96" and "EVOLUTE EXPRESS ABN" for the pretreatment of human blood. We spoke with Professor Makoto Sawada from the Brain Function Division of the Department of Biological Adaptation and Defense Research at Nagoya University’s Institute of Environmental Medicine, who has developed the LMD-MS method and is using the solid-phase extraction column "EVOLUTE EXPRESS ABN" along with the pressurized device "PRESSURE+ 96" for the pretreatment of human cell tissues and blood in his applied research. "EVOLUTE EXPRESS" is a hydrophilic polymer solid phase developed to achieve reliable and dependable solid-phase extraction. "PRESSURE+ 96" is a pressurized sample processing manifold compatible with 96-well plates. 【Overview】 ■ Implementing Institution: Nagoya University Institute of Environmental Medicine, Brain Function Division ■ Implemented Products ・Hydrophilic polymer-based solid-phase extraction column & plate EVOLUTE EXPRESS ・Pressurized sample processing manifold Biotage PRESSURE+ 96 *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This catalog introduces Biotage's ISOLUTE/EVOLUTE sample preparation products, which offer a wide variety of fillers in abundant formats. It features products such as the "ISOLUTE SLE+ non-shaking liquid-liquid extraction diatomaceous earth columns & plates," as well as "ISOLUTE PLD+ protein & phospholipid removal columns & plates" and "ISOLUTE PPT+ protein removal plates." All Biotage products are manufactured under a strict quality control system, ensuring excellent lot-to-lot reproducibility, high target recovery rates, and high purity sample preparation. [Contents (excerpt)] ■ General names of fillers and representative product names ■ Format options ■ ISOLUTE SLE+ non-shaking liquid-liquid extraction diatomaceous earth columns & plates ■ Sample preparation accessories ■ Automated sample preparation devices ■ Concentration devices ■ Price list *For more details, please refer to the PDF materials or feel free to contact us.

• Other physicochemical equipment

This document introduces a case study utilizing the improved silica gel "ISOLUTE SLE+" for the sample preparation of vitamin D analysis using LC-MS/MS. At Chiba University Graduate School of Medicine and its affiliated hospital, the development of a vitamin D analysis method using LC-MS/MS is underway, and products from Biotage, such as "ISOLUTE SLE+", are employed in the sample preparation process. "ISOLUTE SLE+" is a sample preparation product modified for the analysis of biological samples, using a special silica gel that enhances recovery rates and reproducibility through strict particle size control and QC testing. It is based on a liquid-liquid extraction method that removes proteins and phospholipids from biological samples such as plasma and urine, allowing for the extraction of target compounds. [Overview] ■ Implementing Institution: National University Corporation Chiba University ■ Implemented Product: Silica Gel ISOLUTE SLE+ for Biological Sample Preparation *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This document introduces a case study on the implementation of the automatic sample preparation system 'Extrahera' for the preprocessing of large sample volumes. At the Kameyama Laboratory of the Department of Medical Technology, Graduate School of Medicine, Nagoya University, research is currently being conducted primarily to evaluate human exposure to chemical substances through the development and practical application of highly sensitive measurement methods for chemicals in biological samples. We spoke with Associate Professor Jun Kameyama, who introduced 'Extrahera' to accommodate large sample measurements, such as those in large-scale epidemiological studies, about the background of the research and the reasons for introducing the equipment. 'Extrahera' is a compact sample preparation device that adopts pressurized liquid delivery and emphasizes "ease of use." It allows for easy method creation and various condition settings, enabling immediate execution of new sample preparations. [Overview] ■ Implementing Institution: Graduate School of Medicine, Nagoya University ■ Product Introduced: Automatic Sample Preparation Device Biotage Extrahera *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment

This document introduces a case study utilizing the nitrogen blow concentration device "TurboVap LV" for water quality testing through liquid chromatography. The Oita Prefectural Pharmacists Association Testing Center has been using this product for sample concentration in water quality testing of tap water for about 20 years. Recently, a successor model to the "TurboVap LV" has been released, and they have introduced the new model in a replacement of the old one. The "TurboVap LV" is a nitrogen blow concentration device that performs simultaneous and rapid evaporation of multiple samples using its unique gas vortex shearing technology. It is designed for test tubes ranging from 1.5 mL vials to φ30×165 mm, allowing for the concentration of up to 48 samples or 24 samples simultaneously, depending on the size of the test tubes. [Overview] ■ Implementing Institution: Oita Prefectural Pharmacists Association Testing Center ■ Implemented Product: Nitrogen Blow High-Speed Parallel Concentration Device TurboVap LV *For more details, please refer to the PDF document or feel free to contact us.

• Other physicochemical equipment
• Concentration Equipment

At Biotage Japan, we sell the TurboVap, a concentration device that utilizes our unique gas vortex shearing technology to simultaneously evaporate multiple samples at a faster rate than conventional methods, such as heat block and centrifugal types. The TurboVap is a long-selling nitrogen blow type concentration device that has been loved by many users for over 20 years. Biotage has refined the TurboVap and is proposing a new model! Whether you currently own an old TurboVap or are new to TurboVap, we encourage you to consider it! 【Features】 ■ High-efficiency concentration using gas vortex shearing technology ■ Storage and re-execution of concentration conditions ■ Adjustable blow gas pressure gradient settings ■ Detachable blow nozzle ■ Standard drain port ■ Rack design that accommodates test tubes of varying diameters and heights with a single unit *For more details, please request a brochure or download the PDF data.

• Other physicochemical equipment

"Isotera One" is a flash automatic purification system with various features. It has an ultra-high flow rate with purification capacity equivalent to more than two commercially available purification devices, capable of full-wavelength vector scanning without any loss. Additionally, it is attractive for its ease of operation without the need for a manual. The Gradient Optimization function "GO" creates optimal step gradient conditions tailored to the desired peak. 【Features】 ■ Global standard ■ Easy operation without a manual ■ Integrated design that can accommodate gallon bottles ■ Capable of connecting to external detectors such as ELSD and MS ■ Network connection allows results to be output in PDF, CSV, and XML formats *For more details, please refer to the catalog or website.

• Separation device
• Analytical Equipment and Devices
• Other physicochemical equipment

The "Initiator+" is a microwave synthesis device that achieves a reaction temperature of 300°C and a reaction pressure of 30 bar. It offers precise temperature control and reproducible microwave irradiation, making it capable of meeting a wider range of needs. In addition to this product, we have two other products equipped with auto samplers, so please choose according to your application. 【Features】 ■ Accurate temperature control ■ Reproducible microwave irradiation ■ Ideal for reproducing experiments in papers ■ Reaction temperature of 300°C and pressure of 30 bar 【Product Lineup】 ■ Biotage Initiator+ Microwave Synthesis Device ■ Biotage Initiator+Robot Eight - Continuous microwave synthesis device for up to 8 samples ■ Biotage Initiator+Robot Sixty - Continuous microwave synthesis device for up to 60 samples *For more details, please refer to the catalog or feel free to contact us.

• Other physicochemical equipment

The Biotage ELSD (evaporative light-scattering detector) is a universal detector used for purifying organic compounds that cannot be detected by ultraviolet or visible light. When combined with a flash purification system, it can fractionate and purify organic compounds, lipids, steroids, and other substances that lack ultraviolet absorption, which traditional UV detectors cannot detect.

• Separation device
• Other physicochemical equipment
• Analytical Equipment and Devices